First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000300489 |
Resumo: | Abstract Cryptosporidium is a protozoan parasite with a wide range of hosts, including humans. However, only a few Cryptosporidium species have been described in birds (C. meleagridis, C. baileyi, C. galli and C. avium). The aim of this study was to investigate the occurrence of Cryptosporidium spp. in feces of eared doves (Zenaida auriculata), followed by molecular characterization of the parasite. A total of 196 animals of both sexes were trap-captured; the animals were culled and the intestinal contents were collected for DNA extraction. After extraction, a nested-PCR (nPCR), which amplifies a fragment of the 18S rRNA gene of Cryptosporidium spp., was performed. The amplicons obtained were purified and sequenced. PCR analysis revealed that 30 animals (15.3%) were positive for Cryptosporidium spp. There was no significant sex-dependent enrichment of Cryptosporidium occurrence (p > 0.05). Only 15 out of the 30 positive samples were successfully sequenced and their species determined, of which, 13 (86.7%) and 2 (13.3%) were C. meleagridis and C. galli, respectively. Herein, we present for the first time a molecular characterization of Cryptosporidium from feces of eared doves (Z. auriculata) and propose that these birds are a potential source of C. meleagridis infection in humans. |
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First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata)PigeonsepidemiologycryptosporidiosisPCRAbstract Cryptosporidium is a protozoan parasite with a wide range of hosts, including humans. However, only a few Cryptosporidium species have been described in birds (C. meleagridis, C. baileyi, C. galli and C. avium). The aim of this study was to investigate the occurrence of Cryptosporidium spp. in feces of eared doves (Zenaida auriculata), followed by molecular characterization of the parasite. A total of 196 animals of both sexes were trap-captured; the animals were culled and the intestinal contents were collected for DNA extraction. After extraction, a nested-PCR (nPCR), which amplifies a fragment of the 18S rRNA gene of Cryptosporidium spp., was performed. The amplicons obtained were purified and sequenced. PCR analysis revealed that 30 animals (15.3%) were positive for Cryptosporidium spp. There was no significant sex-dependent enrichment of Cryptosporidium occurrence (p > 0.05). Only 15 out of the 30 positive samples were successfully sequenced and their species determined, of which, 13 (86.7%) and 2 (13.3%) were C. meleagridis and C. galli, respectively. Herein, we present for the first time a molecular characterization of Cryptosporidium from feces of eared doves (Z. auriculata) and propose that these birds are a potential source of C. meleagridis infection in humans.Colégio Brasileiro de Parasitologia Veterinária2019-07-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000300489Revista Brasileira de Parasitologia Veterinária v.28 n.3 2019reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-29612019016info:eu-repo/semantics/openAccessSeixas,Mércia deTaroda,AlessandraCardim,Sérgio TosiSasse,João PedroMartins,Thais AgostinhoMartins,Felippe Danyel CardosoMinutti,Ana FláviaVidotto,OdilonBarros,Luiz Daniel deGarcia,João Luiseng2019-09-10T00:00:00Zoai:scielo:S1984-29612019000300489Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2019-09-10T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
title |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
spellingShingle |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) Seixas,Mércia de Pigeons epidemiology cryptosporidiosis PCR |
title_short |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
title_full |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
title_fullStr |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
title_full_unstemmed |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
title_sort |
First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata) |
author |
Seixas,Mércia de |
author_facet |
Seixas,Mércia de Taroda,Alessandra Cardim,Sérgio Tosi Sasse,João Pedro Martins,Thais Agostinho Martins,Felippe Danyel Cardoso Minutti,Ana Flávia Vidotto,Odilon Barros,Luiz Daniel de Garcia,João Luis |
author_role |
author |
author2 |
Taroda,Alessandra Cardim,Sérgio Tosi Sasse,João Pedro Martins,Thais Agostinho Martins,Felippe Danyel Cardoso Minutti,Ana Flávia Vidotto,Odilon Barros,Luiz Daniel de Garcia,João Luis |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Seixas,Mércia de Taroda,Alessandra Cardim,Sérgio Tosi Sasse,João Pedro Martins,Thais Agostinho Martins,Felippe Danyel Cardoso Minutti,Ana Flávia Vidotto,Odilon Barros,Luiz Daniel de Garcia,João Luis |
dc.subject.por.fl_str_mv |
Pigeons epidemiology cryptosporidiosis PCR |
topic |
Pigeons epidemiology cryptosporidiosis PCR |
description |
Abstract Cryptosporidium is a protozoan parasite with a wide range of hosts, including humans. However, only a few Cryptosporidium species have been described in birds (C. meleagridis, C. baileyi, C. galli and C. avium). The aim of this study was to investigate the occurrence of Cryptosporidium spp. in feces of eared doves (Zenaida auriculata), followed by molecular characterization of the parasite. A total of 196 animals of both sexes were trap-captured; the animals were culled and the intestinal contents were collected for DNA extraction. After extraction, a nested-PCR (nPCR), which amplifies a fragment of the 18S rRNA gene of Cryptosporidium spp., was performed. The amplicons obtained were purified and sequenced. PCR analysis revealed that 30 animals (15.3%) were positive for Cryptosporidium spp. There was no significant sex-dependent enrichment of Cryptosporidium occurrence (p > 0.05). Only 15 out of the 30 positive samples were successfully sequenced and their species determined, of which, 13 (86.7%) and 2 (13.3%) were C. meleagridis and C. galli, respectively. Herein, we present for the first time a molecular characterization of Cryptosporidium from feces of eared doves (Z. auriculata) and propose that these birds are a potential source of C. meleagridis infection in humans. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000300489 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019000300489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-29612019016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.28 n.3 2019 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208918337224704 |