Heart remodeling produced by aortic stenosis promotes cardiomyocyte apoptosis mediated by collagen V imbalance

• Autor(a) principal: Sant'Ana, Paula Grippa [UNESP]
• Data de Publicação: 2018
• Outros Autores: Batah, Sabrina Setembre, Leão, Patrícia Santos, Teodoro, Walcy Rosolia, de Souza, Sérgio Luiz Borges [UNESP], Ferreira Mota, Gustavo Augusto [UNESP], Vileigas, Danielle Fernandes [UNESP], da Silva, Vitor Loureiro [UNESP], de Campos, Dijon Henrique Salomé [UNESP], Okoshi, Katashi [UNESP], Capelozzi, Vera Luiza, Cicogna, Antonio Carlos [UNESP], Fabro, Alexandre Todorovic
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• DOI: 10.1016/j.pathophys.2018.07.001
• Texto Completo: http://dx.doi.org/10.1016/j.pathophys.2018.07.001; http://hdl.handle.net/11449/228560
• topic: Aortic stenosis; Apoptosis; Cardiac remodeling; Collagen V

Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload related to changes in both myocyte and extracellular matrix (ECM). We investigated that the imbalance of collagen V promotes cardiomyocyte apoptosis that contributes to heart failure and cell death. Aortic stenosis was induced surgically and male Wistar rats were randomized to 18 weeks (Sham 18 w, n = 12; AoS 18 w, n = 12) and severe of heart failure (Sham HF, n = 12; AoS HF, n = 12) groups. Functional and structural echocardiogram, immunohistochemistry for Ki-67, TUNEL assay and Immunofluorescence for collagen were performed. Our main results were: (1) Progressive reduction of cardiac functional capacity due to cardiac remodeling with decreased eject fraction in heart failure; (2) Imbalance of collagen deposition with increased, crowded and irregular collagen I in situ expression; (3) Dysregulation of dynamic control of collagen fibers with exposed epitopes of collagen V; (4) Additional apoptosis that are dependent to cardiac injury. The collagen V expression in cardiac remodeling is for the first time described and may be related to additional apoptosis and autoimmune response. Our findings suggest a critical role of collagen V in cardiac remodeling to modulate and promote heart failure and death.