Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines

Detalhes bibliográficos
Autor(a) principal: Ferraz, Ana Paula C. R. [UNESP]
Data de Publicação: 2020
Outros Autores: Sussulini, Alessandra, Garcia, Jéssica L. [UNESP], Costa, Mariane R. [UNESP], Francisqueti-Ferron, Fabiane V. [UNESP], Ferron, Artur J. T. [UNESP], Silva, Carol Cristina V. De A. [UNESP], Corrente, José Eduardo [UNESP], Manfio, Vanessa M. [UNESP], Namba, Vickeline [UNESP], Lima, Giuseppina P. P. [UNESP], Pereira, Bismarque S. [UNESP], Fecchio, Denise [UNESP], Minatel, Igor O. [UNESP], Dos Santos, Klinsmann C. [UNESP], Correâ, Camila R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2020/7240216
http://hdl.handle.net/11449/201546
Resumo: Solanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect.
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spelling Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell LinesSolanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect.Saõ Paulo State University (UNESP) Medical SchoolUniversity of Campinas (UNICAMP) Institute of ChemistrySaõ Paulo State University (UNESP) Institute of BiosciencesSaõ Paulo State University (UNESP) Medical SchoolSaõ Paulo State University (UNESP) Institute of BiosciencesUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Ferraz, Ana Paula C. R. [UNESP]Sussulini, AlessandraGarcia, Jéssica L. [UNESP]Costa, Mariane R. [UNESP]Francisqueti-Ferron, Fabiane V. [UNESP]Ferron, Artur J. T. [UNESP]Silva, Carol Cristina V. De A. [UNESP]Corrente, José Eduardo [UNESP]Manfio, Vanessa M. [UNESP]Namba, Vickeline [UNESP]Lima, Giuseppina P. P. [UNESP]Pereira, Bismarque S. [UNESP]Fecchio, Denise [UNESP]Minatel, Igor O. [UNESP]Dos Santos, Klinsmann C. [UNESP]Correâ, Camila R. [UNESP]2020-12-12T02:35:25Z2020-12-12T02:35:25Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2020/7240216Oxidative Medicine and Cellular Longevity, v. 2020.1942-09941942-0900http://hdl.handle.net/11449/20154610.1155/2020/72402162-s2.0-85079210206Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOxidative Medicine and Cellular Longevityinfo:eu-repo/semantics/openAccess2024-09-03T13:14:16Zoai:repositorio.unesp.br:11449/201546Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
title Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
spellingShingle Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
Ferraz, Ana Paula C. R. [UNESP]
title_short Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
title_full Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
title_fullStr Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
title_full_unstemmed Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
title_sort Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
author Ferraz, Ana Paula C. R. [UNESP]
author_facet Ferraz, Ana Paula C. R. [UNESP]
Sussulini, Alessandra
Garcia, Jéssica L. [UNESP]
Costa, Mariane R. [UNESP]
Francisqueti-Ferron, Fabiane V. [UNESP]
Ferron, Artur J. T. [UNESP]
Silva, Carol Cristina V. De A. [UNESP]
Corrente, José Eduardo [UNESP]
Manfio, Vanessa M. [UNESP]
Namba, Vickeline [UNESP]
Lima, Giuseppina P. P. [UNESP]
Pereira, Bismarque S. [UNESP]
Fecchio, Denise [UNESP]
Minatel, Igor O. [UNESP]
Dos Santos, Klinsmann C. [UNESP]
Correâ, Camila R. [UNESP]
author_role author
author2 Sussulini, Alessandra
Garcia, Jéssica L. [UNESP]
Costa, Mariane R. [UNESP]
Francisqueti-Ferron, Fabiane V. [UNESP]
Ferron, Artur J. T. [UNESP]
Silva, Carol Cristina V. De A. [UNESP]
Corrente, José Eduardo [UNESP]
Manfio, Vanessa M. [UNESP]
Namba, Vickeline [UNESP]
Lima, Giuseppina P. P. [UNESP]
Pereira, Bismarque S. [UNESP]
Fecchio, Denise [UNESP]
Minatel, Igor O. [UNESP]
Dos Santos, Klinsmann C. [UNESP]
Correâ, Camila R. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Ferraz, Ana Paula C. R. [UNESP]
Sussulini, Alessandra
Garcia, Jéssica L. [UNESP]
Costa, Mariane R. [UNESP]
Francisqueti-Ferron, Fabiane V. [UNESP]
Ferron, Artur J. T. [UNESP]
Silva, Carol Cristina V. De A. [UNESP]
Corrente, José Eduardo [UNESP]
Manfio, Vanessa M. [UNESP]
Namba, Vickeline [UNESP]
Lima, Giuseppina P. P. [UNESP]
Pereira, Bismarque S. [UNESP]
Fecchio, Denise [UNESP]
Minatel, Igor O. [UNESP]
Dos Santos, Klinsmann C. [UNESP]
Correâ, Camila R. [UNESP]
description Solanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:35:25Z
2020-12-12T02:35:25Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2020/7240216
Oxidative Medicine and Cellular Longevity, v. 2020.
1942-0994
1942-0900
http://hdl.handle.net/11449/201546
10.1155/2020/7240216
2-s2.0-85079210206
url http://dx.doi.org/10.1155/2020/7240216
http://hdl.handle.net/11449/201546
identifier_str_mv Oxidative Medicine and Cellular Longevity, v. 2020.
1942-0994
1942-0900
10.1155/2020/7240216
2-s2.0-85079210206
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oxidative Medicine and Cellular Longevity
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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