Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2020/7240216 http://hdl.handle.net/11449/201546 |
Resumo: | Solanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect. |
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Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell LinesSolanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect.Saõ Paulo State University (UNESP) Medical SchoolUniversity of Campinas (UNICAMP) Institute of ChemistrySaõ Paulo State University (UNESP) Institute of BiosciencesSaõ Paulo State University (UNESP) Medical SchoolSaõ Paulo State University (UNESP) Institute of BiosciencesUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Ferraz, Ana Paula C. R. [UNESP]Sussulini, AlessandraGarcia, Jéssica L. [UNESP]Costa, Mariane R. [UNESP]Francisqueti-Ferron, Fabiane V. [UNESP]Ferron, Artur J. T. [UNESP]Silva, Carol Cristina V. De A. [UNESP]Corrente, José Eduardo [UNESP]Manfio, Vanessa M. [UNESP]Namba, Vickeline [UNESP]Lima, Giuseppina P. P. [UNESP]Pereira, Bismarque S. [UNESP]Fecchio, Denise [UNESP]Minatel, Igor O. [UNESP]Dos Santos, Klinsmann C. [UNESP]Correâ, Camila R. [UNESP]2020-12-12T02:35:25Z2020-12-12T02:35:25Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2020/7240216Oxidative Medicine and Cellular Longevity, v. 2020.1942-09941942-0900http://hdl.handle.net/11449/20154610.1155/2020/72402162-s2.0-85079210206Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOxidative Medicine and Cellular Longevityinfo:eu-repo/semantics/openAccess2024-09-03T13:14:16Zoai:repositorio.unesp.br:11449/201546Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
title |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
spellingShingle |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines Ferraz, Ana Paula C. R. [UNESP] |
title_short |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
title_full |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
title_fullStr |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
title_full_unstemmed |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
title_sort |
Hydroethanolic Extract of Solanum paniculatum L. Fruits Modulates ROS and Cytokine in Human Cell Lines |
author |
Ferraz, Ana Paula C. R. [UNESP] |
author_facet |
Ferraz, Ana Paula C. R. [UNESP] Sussulini, Alessandra Garcia, Jéssica L. [UNESP] Costa, Mariane R. [UNESP] Francisqueti-Ferron, Fabiane V. [UNESP] Ferron, Artur J. T. [UNESP] Silva, Carol Cristina V. De A. [UNESP] Corrente, José Eduardo [UNESP] Manfio, Vanessa M. [UNESP] Namba, Vickeline [UNESP] Lima, Giuseppina P. P. [UNESP] Pereira, Bismarque S. [UNESP] Fecchio, Denise [UNESP] Minatel, Igor O. [UNESP] Dos Santos, Klinsmann C. [UNESP] Correâ, Camila R. [UNESP] |
author_role |
author |
author2 |
Sussulini, Alessandra Garcia, Jéssica L. [UNESP] Costa, Mariane R. [UNESP] Francisqueti-Ferron, Fabiane V. [UNESP] Ferron, Artur J. T. [UNESP] Silva, Carol Cristina V. De A. [UNESP] Corrente, José Eduardo [UNESP] Manfio, Vanessa M. [UNESP] Namba, Vickeline [UNESP] Lima, Giuseppina P. P. [UNESP] Pereira, Bismarque S. [UNESP] Fecchio, Denise [UNESP] Minatel, Igor O. [UNESP] Dos Santos, Klinsmann C. [UNESP] Correâ, Camila R. [UNESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Ferraz, Ana Paula C. R. [UNESP] Sussulini, Alessandra Garcia, Jéssica L. [UNESP] Costa, Mariane R. [UNESP] Francisqueti-Ferron, Fabiane V. [UNESP] Ferron, Artur J. T. [UNESP] Silva, Carol Cristina V. De A. [UNESP] Corrente, José Eduardo [UNESP] Manfio, Vanessa M. [UNESP] Namba, Vickeline [UNESP] Lima, Giuseppina P. P. [UNESP] Pereira, Bismarque S. [UNESP] Fecchio, Denise [UNESP] Minatel, Igor O. [UNESP] Dos Santos, Klinsmann C. [UNESP] Correâ, Camila R. [UNESP] |
description |
Solanum paniculatum L. or popularly known as jurubeba is an herbal medicinal plant. A few studies have investigated its biological effects; however, research aimed at elucidating the redox balance effects from its fruits has not been reported so far. ROS interplays in various fields of medicine such as chemotherapy. Here, we evaluated antioxidant and inflammatory activities of the hydroethanolic extract of Solanum Paniculatum L. (HESPL) fruits in breast cancer cells, as well as its phytochemical profile. The antioxidant profile (carotenoids and phenolic compounds) was obtained by HPLC-DAD-UV and HPLC-APCI-MS. Cancer cell lines and human vein endothelial cells (HUVECs) were cultivated and treated with 1.87-30 μg/mL of HESPL for 24 hrs. Cytotoxicity, oxidative, and inflammation biomarkers were evaluated. The dose of 30 μg/mL of the HESPL extract presented cytotoxicity in the MCF-7 cell line. However, for MDA-MB-231, the cytotoxicity was observed in the dose of 1.87 g/mL. The 1.87 μg/mL and 3.75 μg/mL doses decreased the concentration of IL-6 in MCF-7 cells. In the MDA-MB-231 cells, the HESPL did not decrease the IL-6 concentration; however, in the doses of 15 and 30 μg/mL, an increase in this parameter was observed. The HESPL increased IL-1β concentration in HUVECs. The ROS level in MCF-7 was elevated only at the 30 μg/ml dose. Regarding MDA-MB-231, HESPL promoted increased ROS levels at all doses tested. HUVEC showed no increase in ROS under any dose. HESPL treatment may modulate cytotoxicity, ROS, and cytokine levels due to its phytochemical profile, and it has shown an antioxidant or anti-inflammatory effect. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:25Z 2020-12-12T02:35:25Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2020/7240216 Oxidative Medicine and Cellular Longevity, v. 2020. 1942-0994 1942-0900 http://hdl.handle.net/11449/201546 10.1155/2020/7240216 2-s2.0-85079210206 |
url |
http://dx.doi.org/10.1155/2020/7240216 http://hdl.handle.net/11449/201546 |
identifier_str_mv |
Oxidative Medicine and Cellular Longevity, v. 2020. 1942-0994 1942-0900 10.1155/2020/7240216 2-s2.0-85079210206 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oxidative Medicine and Cellular Longevity |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021359188705280 |