Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection

Detalhes bibliográficos
Autor(a) principal: de Souza, João Carlos [UNESP]
Data de Publicação: 2019
Outros Autores: da Silva, José Luiz [UNESP], Fabrão, Rodrigo Monteiro [UNESP], Stradiotto, Nelson Ramos [UNESP], Zanoni, Maria Valnice Boldrin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.microc.2019.04.010
http://hdl.handle.net/11449/188967
Resumo: The present work describes an easy, rapid and sensitive alternative methodology for simultaneous detection of sugars (glucose, fructose and arabinose), organic acids (gluconic, glucuronic and glucaric acids) and sugar alcohol (arabitol) in wine samples. The method is based on high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) at gold electrode under detection potential of 0.26 V vs Pd|PdO. The optimized chromatographic anion-exchange separation indicates good separation and detection using a binary gradient with 0.28 mol L −1 CH 3 COONa containing 0.10 mol L −1 NaOH and ultrapure water as mobile phase with a flow rate at 1.0 mL min −1 . Analytical curves presented linear relationship from 1.0 × 10 −7 and 1.0 × 10 −3 mol L −1 with detection limits (S/N = 3) from 3.0 × 10 −8 (glucose) to 9.0 × 10 −7 mol L −1 (gluconic acid). The present method was successfully applied in the determination of sugars, organic acids and sugar alcohols in wine, without time consuming pre-treatment of the sample, no need for organic solvent, fast relative execution time, satisfactory separation, high sensitivity and accuracy.
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spelling Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detectionElectrochemical detectionHigh performance anion-exchange chromatographyOrganic acidsSugar alcoholSugarsWineThe present work describes an easy, rapid and sensitive alternative methodology for simultaneous detection of sugars (glucose, fructose and arabinose), organic acids (gluconic, glucuronic and glucaric acids) and sugar alcohol (arabitol) in wine samples. The method is based on high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) at gold electrode under detection potential of 0.26 V vs Pd|PdO. The optimized chromatographic anion-exchange separation indicates good separation and detection using a binary gradient with 0.28 mol L −1 CH 3 COONa containing 0.10 mol L −1 NaOH and ultrapure water as mobile phase with a flow rate at 1.0 mL min −1 . Analytical curves presented linear relationship from 1.0 × 10 −7 and 1.0 × 10 −3 mol L −1 with detection limits (S/N = 3) from 3.0 × 10 −8 (glucose) to 9.0 × 10 −7 mol L −1 (gluconic acid). The present method was successfully applied in the determination of sugars, organic acids and sugar alcohols in wine, without time consuming pre-treatment of the sample, no need for organic solvent, fast relative execution time, satisfactory separation, high sensitivity and accuracy.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) Institute of Chemistry Analytical Chemistry Department National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive (INCT-DATREM), Professor Francisco Degni Street 55São Paulo State University (Unesp) Bioenergy Research Institute, Professor Francisco Degni Street 55School of Industrial Engineering Metallurgical Volta Redonda Fluminense Federal University (UFF), Trabalhadores Avenue 420São Paulo State University (Unesp) Institute of Chemistry Analytical Chemistry Department National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive (INCT-DATREM), Professor Francisco Degni Street 55São Paulo State University (Unesp) Bioenergy Research Institute, Professor Francisco Degni Street 55CNPq: 170176/2017-7FAPESP: 2014/03679-7FAPESP: 2015/12998-1CNPq: 465571/2014-0Universidade Estadual Paulista (Unesp)Fluminense Federal University (UFF)de Souza, João Carlos [UNESP]da Silva, José Luiz [UNESP]Fabrão, Rodrigo Monteiro [UNESP]Stradiotto, Nelson Ramos [UNESP]Zanoni, Maria Valnice Boldrin [UNESP]2019-10-06T16:25:30Z2019-10-06T16:25:30Z2019-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article972-978http://dx.doi.org/10.1016/j.microc.2019.04.010Microchemical Journal, v. 147, p. 972-978.0026-265Xhttp://hdl.handle.net/11449/18896710.1016/j.microc.2019.04.0102-s2.0-850642313050072173018005712Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochemical Journalinfo:eu-repo/semantics/openAccess2021-10-23T20:18:34Zoai:repositorio.unesp.br:11449/188967Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:10:39.997854Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
title Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
spellingShingle Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
de Souza, João Carlos [UNESP]
Electrochemical detection
High performance anion-exchange chromatography
Organic acids
Sugar alcohol
Sugars
Wine
title_short Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
title_full Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
title_fullStr Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
title_full_unstemmed Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
title_sort Electroactive sugars, organic acids and sugar alcohol analysis in wine using anion-exchange chromatography with electrochemical detection
author de Souza, João Carlos [UNESP]
author_facet de Souza, João Carlos [UNESP]
da Silva, José Luiz [UNESP]
Fabrão, Rodrigo Monteiro [UNESP]
Stradiotto, Nelson Ramos [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
author_role author
author2 da Silva, José Luiz [UNESP]
Fabrão, Rodrigo Monteiro [UNESP]
Stradiotto, Nelson Ramos [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Fluminense Federal University (UFF)
dc.contributor.author.fl_str_mv de Souza, João Carlos [UNESP]
da Silva, José Luiz [UNESP]
Fabrão, Rodrigo Monteiro [UNESP]
Stradiotto, Nelson Ramos [UNESP]
Zanoni, Maria Valnice Boldrin [UNESP]
dc.subject.por.fl_str_mv Electrochemical detection
High performance anion-exchange chromatography
Organic acids
Sugar alcohol
Sugars
Wine
topic Electrochemical detection
High performance anion-exchange chromatography
Organic acids
Sugar alcohol
Sugars
Wine
description The present work describes an easy, rapid and sensitive alternative methodology for simultaneous detection of sugars (glucose, fructose and arabinose), organic acids (gluconic, glucuronic and glucaric acids) and sugar alcohol (arabitol) in wine samples. The method is based on high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) at gold electrode under detection potential of 0.26 V vs Pd|PdO. The optimized chromatographic anion-exchange separation indicates good separation and detection using a binary gradient with 0.28 mol L −1 CH 3 COONa containing 0.10 mol L −1 NaOH and ultrapure water as mobile phase with a flow rate at 1.0 mL min −1 . Analytical curves presented linear relationship from 1.0 × 10 −7 and 1.0 × 10 −3 mol L −1 with detection limits (S/N = 3) from 3.0 × 10 −8 (glucose) to 9.0 × 10 −7 mol L −1 (gluconic acid). The present method was successfully applied in the determination of sugars, organic acids and sugar alcohols in wine, without time consuming pre-treatment of the sample, no need for organic solvent, fast relative execution time, satisfactory separation, high sensitivity and accuracy.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:25:30Z
2019-10-06T16:25:30Z
2019-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.microc.2019.04.010
Microchemical Journal, v. 147, p. 972-978.
0026-265X
http://hdl.handle.net/11449/188967
10.1016/j.microc.2019.04.010
2-s2.0-85064231305
0072173018005712
url http://dx.doi.org/10.1016/j.microc.2019.04.010
http://hdl.handle.net/11449/188967
identifier_str_mv Microchemical Journal, v. 147, p. 972-978.
0026-265X
10.1016/j.microc.2019.04.010
2-s2.0-85064231305
0072173018005712
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microchemical Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 972-978
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128474605420544