Conidial alkaline phosphatase from Neurospora crassa
Autor(a) principal: | |
---|---|
Data de Publicação: | 1996 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/0031-9422(95)00534-X http://hdl.handle.net/11449/224033 |
Resumo: | An alkaline phosphatase was purified from conidia of a Neurospora crassa wild type strain. The Mr of the purified native enzyme was estimated as ca 145000 and 110000 by gel filtration, in the presence and absence of magnesium ions, respectively. A single polypeptide band of Mr 36 000 was detected by SDS-PAGE, suggesting that the native enzyme was a tetramer of apparently identical subunits. Conidial alkaline phosphatase was an acidic protein (pl = 4.0 ± 0.1), with 40% carbohydrate content. Optimal pH was affected by substrate concentration and magnesium ions. Low concentrations of calcium ions (0.1 mM) had slight stimulatory effects, but in excess (5 mM) caused protein aggregates with decreased activity. The enzyme specificity against different substrates was compared with those reported for constitutive or Pi-repressible alkaline phosphatases produced by N. crassa. The results suggested that the conidial alkaline phosphatase represented a different class among other such enzymes synthesized by this organism. Copyright © 1994 Elsevier Science Ltd. All rights reserved. |
id |
UNSP_5d7978bd5900fc44f1c2f116c101693d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/224033 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Conidial alkaline phosphatase from Neurospora crassaAlkaline phosphataseConidial enzymesHydrophobic chromatographyNeurospora crassaP-nitrophenyl phosphateAn alkaline phosphatase was purified from conidia of a Neurospora crassa wild type strain. The Mr of the purified native enzyme was estimated as ca 145000 and 110000 by gel filtration, in the presence and absence of magnesium ions, respectively. A single polypeptide band of Mr 36 000 was detected by SDS-PAGE, suggesting that the native enzyme was a tetramer of apparently identical subunits. Conidial alkaline phosphatase was an acidic protein (pl = 4.0 ± 0.1), with 40% carbohydrate content. Optimal pH was affected by substrate concentration and magnesium ions. Low concentrations of calcium ions (0.1 mM) had slight stimulatory effects, but in excess (5 mM) caused protein aggregates with decreased activity. The enzyme specificity against different substrates was compared with those reported for constitutive or Pi-repressible alkaline phosphatases produced by N. crassa. The results suggested that the conidial alkaline phosphatase represented a different class among other such enzymes synthesized by this organism. Copyright © 1994 Elsevier Science Ltd. All rights reserved.Departamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto-USP, 14040-901-Ribeirão Preto, SPDepartamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto-USP, 14040-901-Ribeirão Preto, SPDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal-UNESP, Jaboticabal, SPDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal-UNESP, Jaboticabal, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Say, José C.Furriel, Rosa P.M.Ciancaglini, PietroJorge, João A.Lourdes, MariaPolizeli, T. M.Pizauro, João M. [UNESP]Terenzi, Héctor F.Leone, Francisco A.2022-04-28T19:54:23Z2022-04-28T19:54:23Z1996-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article71-75http://dx.doi.org/10.1016/0031-9422(95)00534-XPhytochemistry, v. 41, n. 1, p. 71-75, 1996.0031-9422http://hdl.handle.net/11449/22403310.1016/0031-9422(95)00534-X2-s2.0-0030019896Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytochemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:54:23Zoai:repositorio.unesp.br:11449/224033Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:54:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Conidial alkaline phosphatase from Neurospora crassa |
title |
Conidial alkaline phosphatase from Neurospora crassa |
spellingShingle |
Conidial alkaline phosphatase from Neurospora crassa Say, José C. Alkaline phosphatase Conidial enzymes Hydrophobic chromatography Neurospora crassa P-nitrophenyl phosphate |
title_short |
Conidial alkaline phosphatase from Neurospora crassa |
title_full |
Conidial alkaline phosphatase from Neurospora crassa |
title_fullStr |
Conidial alkaline phosphatase from Neurospora crassa |
title_full_unstemmed |
Conidial alkaline phosphatase from Neurospora crassa |
title_sort |
Conidial alkaline phosphatase from Neurospora crassa |
author |
Say, José C. |
author_facet |
Say, José C. Furriel, Rosa P.M. Ciancaglini, Pietro Jorge, João A. Lourdes, Maria Polizeli, T. M. Pizauro, João M. [UNESP] Terenzi, Héctor F. Leone, Francisco A. |
author_role |
author |
author2 |
Furriel, Rosa P.M. Ciancaglini, Pietro Jorge, João A. Lourdes, Maria Polizeli, T. M. Pizauro, João M. [UNESP] Terenzi, Héctor F. Leone, Francisco A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Say, José C. Furriel, Rosa P.M. Ciancaglini, Pietro Jorge, João A. Lourdes, Maria Polizeli, T. M. Pizauro, João M. [UNESP] Terenzi, Héctor F. Leone, Francisco A. |
dc.subject.por.fl_str_mv |
Alkaline phosphatase Conidial enzymes Hydrophobic chromatography Neurospora crassa P-nitrophenyl phosphate |
topic |
Alkaline phosphatase Conidial enzymes Hydrophobic chromatography Neurospora crassa P-nitrophenyl phosphate |
description |
An alkaline phosphatase was purified from conidia of a Neurospora crassa wild type strain. The Mr of the purified native enzyme was estimated as ca 145000 and 110000 by gel filtration, in the presence and absence of magnesium ions, respectively. A single polypeptide band of Mr 36 000 was detected by SDS-PAGE, suggesting that the native enzyme was a tetramer of apparently identical subunits. Conidial alkaline phosphatase was an acidic protein (pl = 4.0 ± 0.1), with 40% carbohydrate content. Optimal pH was affected by substrate concentration and magnesium ions. Low concentrations of calcium ions (0.1 mM) had slight stimulatory effects, but in excess (5 mM) caused protein aggregates with decreased activity. The enzyme specificity against different substrates was compared with those reported for constitutive or Pi-repressible alkaline phosphatases produced by N. crassa. The results suggested that the conidial alkaline phosphatase represented a different class among other such enzymes synthesized by this organism. Copyright © 1994 Elsevier Science Ltd. All rights reserved. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-01-01 2022-04-28T19:54:23Z 2022-04-28T19:54:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/0031-9422(95)00534-X Phytochemistry, v. 41, n. 1, p. 71-75, 1996. 0031-9422 http://hdl.handle.net/11449/224033 10.1016/0031-9422(95)00534-X 2-s2.0-0030019896 |
url |
http://dx.doi.org/10.1016/0031-9422(95)00534-X http://hdl.handle.net/11449/224033 |
identifier_str_mv |
Phytochemistry, v. 41, n. 1, p. 71-75, 1996. 0031-9422 10.1016/0031-9422(95)00534-X 2-s2.0-0030019896 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
71-75 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790311911522304 |