Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/219894 |
Resumo: | The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screwcap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 °C for 30 min and kept at -196 °C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 °C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 °C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 °C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
id |
UNSP_5dd3d6cfaade36e5ba754dce72672f33 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/219894 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetorCryopreservation of agaricus blazei in liquid nitrogen using dmso as cryoprotectantAgaricus brasiliensis. agaricus subrufescens. thawing. freezing. viability. preservationThe preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screwcap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 °C for 30 min and kept at -196 °C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 °C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 °C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 °C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation.Laboratório de Biologia Molecular Universidade Paranaense - UNIPAR, Umuarama, PRUniversidade Estadual Paulista - UNESP, Botucatu, SPUniversidade Estadual Paulista - UNESP, Botucatu, SPUniversidade Paranaense - UNIPARUniversidade Estadual Paulista (UNESP)Colauto, Nelson BarrosDa Eira, Augusto Ferreira [UNESP]Linde, Giani Andrea2022-04-28T18:58:13Z2022-04-28T18:58:13Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1034-1037Bioscience Journal, v. 28, n. 6, p. 1034-1037, 2012.1516-37251981-3163http://hdl.handle.net/11449/2198942-s2.0-84875194937Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporBioscience Journalinfo:eu-repo/semantics/openAccess2022-04-28T18:58:13Zoai:repositorio.unesp.br:11449/219894Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:23:48.177320Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor Cryopreservation of agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
title |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
spellingShingle |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor Colauto, Nelson Barros Agaricus brasiliensis. agaricus subrufescens. thawing. freezing. viability. preservation |
title_short |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
title_full |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
title_fullStr |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
title_full_unstemmed |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
title_sort |
Criopreservação de agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
author |
Colauto, Nelson Barros |
author_facet |
Colauto, Nelson Barros Da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
author_role |
author |
author2 |
Da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Paranaense - UNIPAR Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Colauto, Nelson Barros Da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
dc.subject.por.fl_str_mv |
Agaricus brasiliensis. agaricus subrufescens. thawing. freezing. viability. preservation |
topic |
Agaricus brasiliensis. agaricus subrufescens. thawing. freezing. viability. preservation |
description |
The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screwcap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 °C for 30 min and kept at -196 °C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 °C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 °C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 °C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2022-04-28T18:58:13Z 2022-04-28T18:58:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Bioscience Journal, v. 28, n. 6, p. 1034-1037, 2012. 1516-3725 1981-3163 http://hdl.handle.net/11449/219894 2-s2.0-84875194937 |
identifier_str_mv |
Bioscience Journal, v. 28, n. 6, p. 1034-1037, 2012. 1516-3725 1981-3163 2-s2.0-84875194937 |
url |
http://hdl.handle.net/11449/219894 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Bioscience Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1034-1037 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128642779185152 |