Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2015.09.008 http://hdl.handle.net/11449/172284 |
Resumo: | Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice. |
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Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller micePCV2RodentsSwine virusViral loadMice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice.Departamento de Medicina Veterinária Preventiva e Saúde Animal Da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87Complexo Educacional Faculdades Metropolitana Unidas Veterinária, Rua Ministro Nelson Hungria, 541, Real ParqueDepartamento de Clínica e Cirurgia Veterinária Escola de Veterinária Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - PampulhaDepartamento de Imunologia e Microbiologia Instituto de Biociência Universidade Estadual Paulista Julio de Mesquita Filho, Campus de BotucatuBiotechnology Inst. - IBTEC Sao Paulo State Univ. UNESP, Campus de BotucatuDepartamento de Imunologia e Microbiologia Instituto de Biociência Universidade Estadual Paulista Julio de Mesquita Filho, Campus de BotucatuBiotechnology Inst. - IBTEC Sao Paulo State Univ. UNESP, Campus de BotucatuUniversidade de São Paulo (USP)VeterináriaUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)de Castro, Alessandra M.M.G.Cruz, Taís F. [UNESP]Yamada, Katarina B.Gerber, Priscilla F.Gabardo, Michelle P.Araújo, João P. [UNESP]Guedes, Roberto M.C.Mori, Cinthia K.Oliveira, Camila P.Santos, Sueli S.Richtzenhain, Leonardo J.2018-12-11T16:59:31Z2018-12-11T16:59:31Z2015-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article70-72application/pdfhttp://dx.doi.org/10.1016/j.rvsc.2015.09.008Research in Veterinary Science, v. 103, p. 70-72.1532-26610034-5288http://hdl.handle.net/11449/17228410.1016/j.rvsc.2015.09.0082-s2.0-849495639612-s2.0-84949563961.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Science0,593info:eu-repo/semantics/openAccess2023-12-27T06:24:33Zoai:repositorio.unesp.br:11449/172284Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-27T06:24:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
title |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
spellingShingle |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice de Castro, Alessandra M.M.G. PCV2 Rodents Swine virus Viral load |
title_short |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
title_full |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
title_fullStr |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
title_full_unstemmed |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
title_sort |
Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice |
author |
de Castro, Alessandra M.M.G. |
author_facet |
de Castro, Alessandra M.M.G. Cruz, Taís F. [UNESP] Yamada, Katarina B. Gerber, Priscilla F. Gabardo, Michelle P. Araújo, João P. [UNESP] Guedes, Roberto M.C. Mori, Cinthia K. Oliveira, Camila P. Santos, Sueli S. Richtzenhain, Leonardo J. |
author_role |
author |
author2 |
Cruz, Taís F. [UNESP] Yamada, Katarina B. Gerber, Priscilla F. Gabardo, Michelle P. Araújo, João P. [UNESP] Guedes, Roberto M.C. Mori, Cinthia K. Oliveira, Camila P. Santos, Sueli S. Richtzenhain, Leonardo J. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Veterinária Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Castro, Alessandra M.M.G. Cruz, Taís F. [UNESP] Yamada, Katarina B. Gerber, Priscilla F. Gabardo, Michelle P. Araújo, João P. [UNESP] Guedes, Roberto M.C. Mori, Cinthia K. Oliveira, Camila P. Santos, Sueli S. Richtzenhain, Leonardo J. |
dc.subject.por.fl_str_mv |
PCV2 Rodents Swine virus Viral load |
topic |
PCV2 Rodents Swine virus Viral load |
description |
Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 2018-12-11T16:59:31Z 2018-12-11T16:59:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2015.09.008 Research in Veterinary Science, v. 103, p. 70-72. 1532-2661 0034-5288 http://hdl.handle.net/11449/172284 10.1016/j.rvsc.2015.09.008 2-s2.0-84949563961 2-s2.0-84949563961.pdf |
url |
http://dx.doi.org/10.1016/j.rvsc.2015.09.008 http://hdl.handle.net/11449/172284 |
identifier_str_mv |
Research in Veterinary Science, v. 103, p. 70-72. 1532-2661 0034-5288 10.1016/j.rvsc.2015.09.008 2-s2.0-84949563961 2-s2.0-84949563961.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science 0,593 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
70-72 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965428561215488 |