Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/15287394.2017.1420505 http://hdl.handle.net/11449/164042 |
Resumo: | In view of the biological activities and growing therapeutic interest in oleoresin obtained from Copaifera multijuga, this study aimed to determine the genotoxic and antigenotoxic potential of this oleoresin (CMO) and its chemical marker, diterpene (-)-copalic acid (CA). The micronucleus (MN) assay in V79 cell cultures and the Ames test were used for in vitro analyses, as well as MN and comet assays in Swiss mice for in vivo analyses. The in vitro genotoxicity/mutagenicity results showed that either CMO (30, 60, or 120 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) or CA (2.42; 4.84, or 9.7 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) did not induce a significant effect on the frequency of MN and number of revertants, demonstrating an absence of genotoxic and mutagenic activities, respectively, in vitro. In contrast, these natural products significantly reduced the frequency of MN induced by methyl methanesulfonate (MMS), and exerted a marked inhibitory effect against indirect-acting mutagens in the Ames test. In the in vivo test system, animals treated with CMO (6.25 mg/kg b.w.) exhibited a significant decrease in rate of MN occurrence compared to those treated only with MMS. An antigenotoxic effect of CA was noted in the MN test (1 and 2 mg/kg b.w.) and the comet assay (0.5 mg/kg b.w.). Data suggest that the chemical marker of the genus Copaifera, CA, may partially be responsible for the observed chemopreventive effect attributed to CMO exposure. |
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Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acidCopaifera multijuga Hayne(-)-copalic acidgenotoxicityantigenotoxicitymicronucleus testAmes testcomet assayIn view of the biological activities and growing therapeutic interest in oleoresin obtained from Copaifera multijuga, this study aimed to determine the genotoxic and antigenotoxic potential of this oleoresin (CMO) and its chemical marker, diterpene (-)-copalic acid (CA). The micronucleus (MN) assay in V79 cell cultures and the Ames test were used for in vitro analyses, as well as MN and comet assays in Swiss mice for in vivo analyses. The in vitro genotoxicity/mutagenicity results showed that either CMO (30, 60, or 120 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) or CA (2.42; 4.84, or 9.7 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) did not induce a significant effect on the frequency of MN and number of revertants, demonstrating an absence of genotoxic and mutagenic activities, respectively, in vitro. In contrast, these natural products significantly reduced the frequency of MN induced by methyl methanesulfonate (MMS), and exerted a marked inhibitory effect against indirect-acting mutagens in the Ames test. In the in vivo test system, animals treated with CMO (6.25 mg/kg b.w.) exhibited a significant decrease in rate of MN occurrence compared to those treated only with MMS. An antigenotoxic effect of CA was noted in the MN test (1 and 2 mg/kg b.w.) and the comet assay (0.5 mg/kg b.w.). Data suggest that the chemical marker of the genus Copaifera, CA, may partially be responsible for the observed chemopreventive effect attributed to CMO exposure.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Franca, Dept Ciencias, BR-14404600 Franca, SP, BrazilUniv Araraquara, Grp Pesquisa Quim Med & Med Regenerat, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, Araraquara, SP, BrazilFAPESP: 2011/13630-7FAPESP: 2009/17237-8Taylor & Francis IncUniv FrancaUniv AraraquaraUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Alves, Jacqueline MoraisLeandro, Luis FernandoSenedese, Juliana MarquesCastro, Pamela Tinti dePereira, Daiane EleuterioResende, Flavia AparecidaCampos, Debora Leite [UNESP]Mangabeira da Silva, Jonas JoaquimVaranda, Eliana Aparecida [UNESP]Bastos, Jairo KenuppAmbrosio, Sergio RicardoTavares, Denise Crispim2018-11-26T17:48:53Z2018-11-26T17:48:53Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article116-129application/pdfhttp://dx.doi.org/10.1080/15287394.2017.1420505Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 81, n. 5, p. 116-129, 2018.1528-7394http://hdl.handle.net/11449/16404210.1080/15287394.2017.1420505WOS:000428738900004WOS000428738900004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Toxicology And Environmental Health-part A-current Issues0,888info:eu-repo/semantics/openAccess2024-06-24T13:08:01Zoai:repositorio.unesp.br:11449/164042Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:45:14.500172Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
title |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
spellingShingle |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid Alves, Jacqueline Morais Copaifera multijuga Hayne (-)-copalic acid genotoxicity antigenotoxicity micronucleus test Ames test comet assay |
title_short |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
title_full |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
title_fullStr |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
title_full_unstemmed |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
title_sort |
Antigenotoxicity properties of Copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid |
author |
Alves, Jacqueline Morais |
author_facet |
Alves, Jacqueline Morais Leandro, Luis Fernando Senedese, Juliana Marques Castro, Pamela Tinti de Pereira, Daiane Eleuterio Resende, Flavia Aparecida Campos, Debora Leite [UNESP] Mangabeira da Silva, Jonas Joaquim Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Ambrosio, Sergio Ricardo Tavares, Denise Crispim |
author_role |
author |
author2 |
Leandro, Luis Fernando Senedese, Juliana Marques Castro, Pamela Tinti de Pereira, Daiane Eleuterio Resende, Flavia Aparecida Campos, Debora Leite [UNESP] Mangabeira da Silva, Jonas Joaquim Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Ambrosio, Sergio Ricardo Tavares, Denise Crispim |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Franca Univ Araraquara Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Alves, Jacqueline Morais Leandro, Luis Fernando Senedese, Juliana Marques Castro, Pamela Tinti de Pereira, Daiane Eleuterio Resende, Flavia Aparecida Campos, Debora Leite [UNESP] Mangabeira da Silva, Jonas Joaquim Varanda, Eliana Aparecida [UNESP] Bastos, Jairo Kenupp Ambrosio, Sergio Ricardo Tavares, Denise Crispim |
dc.subject.por.fl_str_mv |
Copaifera multijuga Hayne (-)-copalic acid genotoxicity antigenotoxicity micronucleus test Ames test comet assay |
topic |
Copaifera multijuga Hayne (-)-copalic acid genotoxicity antigenotoxicity micronucleus test Ames test comet assay |
description |
In view of the biological activities and growing therapeutic interest in oleoresin obtained from Copaifera multijuga, this study aimed to determine the genotoxic and antigenotoxic potential of this oleoresin (CMO) and its chemical marker, diterpene (-)-copalic acid (CA). The micronucleus (MN) assay in V79 cell cultures and the Ames test were used for in vitro analyses, as well as MN and comet assays in Swiss mice for in vivo analyses. The in vitro genotoxicity/mutagenicity results showed that either CMO (30, 60, or 120 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) or CA (2.42; 4.84, or 9.7 mu g/ml-MN assay; 0.39-3.12 mg/plate-Ames test) did not induce a significant effect on the frequency of MN and number of revertants, demonstrating an absence of genotoxic and mutagenic activities, respectively, in vitro. In contrast, these natural products significantly reduced the frequency of MN induced by methyl methanesulfonate (MMS), and exerted a marked inhibitory effect against indirect-acting mutagens in the Ames test. In the in vivo test system, animals treated with CMO (6.25 mg/kg b.w.) exhibited a significant decrease in rate of MN occurrence compared to those treated only with MMS. An antigenotoxic effect of CA was noted in the MN test (1 and 2 mg/kg b.w.) and the comet assay (0.5 mg/kg b.w.). Data suggest that the chemical marker of the genus Copaifera, CA, may partially be responsible for the observed chemopreventive effect attributed to CMO exposure. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:48:53Z 2018-11-26T17:48:53Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/15287394.2017.1420505 Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 81, n. 5, p. 116-129, 2018. 1528-7394 http://hdl.handle.net/11449/164042 10.1080/15287394.2017.1420505 WOS:000428738900004 WOS000428738900004.pdf |
url |
http://dx.doi.org/10.1080/15287394.2017.1420505 http://hdl.handle.net/11449/164042 |
identifier_str_mv |
Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 81, n. 5, p. 116-129, 2018. 1528-7394 10.1080/15287394.2017.1420505 WOS:000428738900004 WOS000428738900004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Toxicology And Environmental Health-part A-current Issues 0,888 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
116-129 application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis Inc |
publisher.none.fl_str_mv |
Taylor & Francis Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129242392690688 |