Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway

Detalhes bibliográficos
Autor(a) principal: Ferron, Artur Junio Togneri
Data de Publicação: 2015
Outros Autores: Jacobsen, Bruno Barcellos, Sant'Ana, Paula Grippa [UNESP], Campos, Dijon Henrique Salomé de [UNESP], Tomasi, Loreta Casquel de [UNESP], Luvizotto, Renata de Azevedo Mello, Cicogna, Antonio Carlos [UNESP], Leopoldo, André Soares, Lima-Leopoldo, Ana Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0138605
http://hdl.handle.net/11449/131088
Resumo: Obesity has been shown to impair myocardial performance. Some factors have been suggested as responsible for possible cardiac abnormalities in models of obesity, among them beta-adrenergic (βA) system, an important mechanism of regulation of myocardial contraction and relaxation. The objective of present study was to evaluate the involvement of βA system components in myocardial dysfunction induced by obesity. Thirty-day-old male Wistar rats were distributed in control (C, n = 25) and obese (Ob, n = 25) groups. The C group was fed a standard diet and Ob group was fed four unsaturated high-fat diets for 15 weeks. Cardiac function was evaluated by isolated papillary muscle preparation and βA system evaluated by using cumulative concentrations of isoproterenol and Western blot. After 15 weeks, the Ob rats developed higher adiposity index than C rats and several comorbidities; however, were not associated with changes in systolic blood pressure. Obesity caused structural changes and the myocardial responsiveness to post-rest contraction stimulus and increased extracellular calcium (Ca2+) was compromised. There were no changes in cardiac function between groups after βA stimulation. The obesity was not accompanied by changes in protein expression of G protein subunit alpha (Gsα) and βA receptors (β1AR and β2AR). In conclusion, the myocardial dysfunction caused by unsaturated high-fat diet-induced obesity, after 15 weeks, is not related to βAR system impairment at the receptor-signalling pathway.
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spelling Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathwayObesity has been shown to impair myocardial performance. Some factors have been suggested as responsible for possible cardiac abnormalities in models of obesity, among them beta-adrenergic (βA) system, an important mechanism of regulation of myocardial contraction and relaxation. The objective of present study was to evaluate the involvement of βA system components in myocardial dysfunction induced by obesity. Thirty-day-old male Wistar rats were distributed in control (C, n = 25) and obese (Ob, n = 25) groups. The C group was fed a standard diet and Ob group was fed four unsaturated high-fat diets for 15 weeks. Cardiac function was evaluated by isolated papillary muscle preparation and βA system evaluated by using cumulative concentrations of isoproterenol and Western blot. After 15 weeks, the Ob rats developed higher adiposity index than C rats and several comorbidities; however, were not associated with changes in systolic blood pressure. Obesity caused structural changes and the myocardial responsiveness to post-rest contraction stimulus and increased extracellular calcium (Ca2+) was compromised. There were no changes in cardiac function between groups after βA stimulation. The obesity was not accompanied by changes in protein expression of G protein subunit alpha (Gsα) and βA receptors (β1AR and β2AR). In conclusion, the myocardial dysfunction caused by unsaturated high-fat diet-induced obesity, after 15 weeks, is not related to βAR system impairment at the receptor-signalling pathway.Fundação de Apoio à Pesquisa e Estudos na Área da Saúde (FAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Center of Physical Education and Sports, Department of Sports, Federal University of Espírito Santo, Vitória, Espírito Santo, BrazilDepartment of Clinical and Cardiology, School of Medicine, UNESP- Univ. Estadual Paulista, Botucatu, São Paulo, BrazilInstituto de Ciências da Saúde, Federal University of Mato Grosso, Sinop, Mato Grosso, BrazilDepartment of Clinical and Cardiology, School of Medicine, UNESP- Univ. Estadual Paulista, Botucatu, São Paulo, BrazilFAPES: 53618009/2011FAPESP: 2012/16647-0Public Library ScienceUniversidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (Unesp)Universidade Federal do Mato Grosso (UFMT)Ferron, Artur Junio TogneriJacobsen, Bruno BarcellosSant'Ana, Paula Grippa [UNESP]Campos, Dijon Henrique Salomé de [UNESP]Tomasi, Loreta Casquel de [UNESP]Luvizotto, Renata de Azevedo MelloCicogna, Antonio Carlos [UNESP]Leopoldo, André SoaresLima-Leopoldo, Ana Paula2015-12-07T15:31:29Z2015-12-07T15:31:29Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0138605Plos One, v. 10, n. 9, 2015.1932-6203http://hdl.handle.net/11449/13108810.1371/journal.pone.0138605PMC4577087.pdf941897010356413726390297PMC4577087PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2024-08-14T17:23:09Zoai:repositorio.unesp.br:11449/131088Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
title Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
spellingShingle Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
Ferron, Artur Junio Togneri
title_short Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
title_full Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
title_fullStr Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
title_full_unstemmed Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
title_sort Cardiac dysfunction induced by obesity es not related to beta-adrenergic system impairment at the receptor-signalling pathway
author Ferron, Artur Junio Togneri
author_facet Ferron, Artur Junio Togneri
Jacobsen, Bruno Barcellos
Sant'Ana, Paula Grippa [UNESP]
Campos, Dijon Henrique Salomé de [UNESP]
Tomasi, Loreta Casquel de [UNESP]
Luvizotto, Renata de Azevedo Mello
Cicogna, Antonio Carlos [UNESP]
Leopoldo, André Soares
Lima-Leopoldo, Ana Paula
author_role author
author2 Jacobsen, Bruno Barcellos
Sant'Ana, Paula Grippa [UNESP]
Campos, Dijon Henrique Salomé de [UNESP]
Tomasi, Loreta Casquel de [UNESP]
Luvizotto, Renata de Azevedo Mello
Cicogna, Antonio Carlos [UNESP]
Leopoldo, André Soares
Lima-Leopoldo, Ana Paula
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Espírito Santo (UFES)
Universidade Estadual Paulista (Unesp)
Universidade Federal do Mato Grosso (UFMT)
dc.contributor.author.fl_str_mv Ferron, Artur Junio Togneri
Jacobsen, Bruno Barcellos
Sant'Ana, Paula Grippa [UNESP]
Campos, Dijon Henrique Salomé de [UNESP]
Tomasi, Loreta Casquel de [UNESP]
Luvizotto, Renata de Azevedo Mello
Cicogna, Antonio Carlos [UNESP]
Leopoldo, André Soares
Lima-Leopoldo, Ana Paula
description Obesity has been shown to impair myocardial performance. Some factors have been suggested as responsible for possible cardiac abnormalities in models of obesity, among them beta-adrenergic (βA) system, an important mechanism of regulation of myocardial contraction and relaxation. The objective of present study was to evaluate the involvement of βA system components in myocardial dysfunction induced by obesity. Thirty-day-old male Wistar rats were distributed in control (C, n = 25) and obese (Ob, n = 25) groups. The C group was fed a standard diet and Ob group was fed four unsaturated high-fat diets for 15 weeks. Cardiac function was evaluated by isolated papillary muscle preparation and βA system evaluated by using cumulative concentrations of isoproterenol and Western blot. After 15 weeks, the Ob rats developed higher adiposity index than C rats and several comorbidities; however, were not associated with changes in systolic blood pressure. Obesity caused structural changes and the myocardial responsiveness to post-rest contraction stimulus and increased extracellular calcium (Ca2+) was compromised. There were no changes in cardiac function between groups after βA stimulation. The obesity was not accompanied by changes in protein expression of G protein subunit alpha (Gsα) and βA receptors (β1AR and β2AR). In conclusion, the myocardial dysfunction caused by unsaturated high-fat diet-induced obesity, after 15 weeks, is not related to βAR system impairment at the receptor-signalling pathway.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-07T15:31:29Z
2015-12-07T15:31:29Z
2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0138605
Plos One, v. 10, n. 9, 2015.
1932-6203
http://hdl.handle.net/11449/131088
10.1371/journal.pone.0138605
PMC4577087.pdf
9418970103564137
26390297
PMC4577087
url http://dx.doi.org/10.1371/journal.pone.0138605
http://hdl.handle.net/11449/131088
identifier_str_mv Plos One, v. 10, n. 9, 2015.
1932-6203
10.1371/journal.pone.0138605
PMC4577087.pdf
9418970103564137
26390297
PMC4577087
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos One
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv PubMed
reponame:Repositório Institucional da UNESP
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instname_str Universidade Estadual Paulista (UNESP)
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