Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs

Detalhes bibliográficos
Autor(a) principal: Escalante-Rojas, Maria C.
Data de Publicação: 2021
Outros Autores: Tolussi, Carlos E., Gomes, Aline D., Munoz-Penuela, Marcela, Brambila-Souza, Gabriela [UNESP], Branco, Giovana S. [UNESP], Moreira, Renata G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ecoenv.2020.111502
http://hdl.handle.net/11449/209837
Resumo: The reproductive physiology of fish can be changed by the presence of pollutants in the water, which act as endocrine disrupting compounds (EDC). We evaluated the impacts of water contaminants in polluted reservoirs acting as possible EDC on the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males. We used biomarkers with different levels of biological organization. Hoplias malabaricus adult males were collected in the summer and winter at five different sites in the Tiete River Basin: the Ponte Nova reservoir (PN), considered a reference site due to the low anthropogenic influence; the Billings reservoir (BIL) at two different branches; and the Guarapiranga reservoir (GUA) at two different branches. Astyanax fasciatus adult males were collected at PN and BIL. BIL and GUA are subjected to great anthropogenic action. We analyzed gonadal histomorphology, testosterone (T), 11-ketotestosterone (11-KT), estradiol (E2) plasma levels, and gene expression of hepatic vitellogenin (vtgA) and pituitary follicle stimulating hormone (fsh beta). In the PN reservoir (reference), the biomarkers analyzed in both species did not differ between the periods analyzed. This is an evidence that the animals keep the same reproductive activity during both seasons. The changes in the plasma concentration of gonadal steroids in both species in polluted reservoirs suggest the presence of EDC compounds in the water and/ or adjusts of the physiological setpoint to allow the reproduction in such adverse conditions. The use of vtgA as biomarker suggests the presence of estrogenic compounds, mainly in BIL, but with a more evident response of H. malabaricus. However, even considering physiological changes, both species present testes during the maturation phase that allow the reproduction in an environment with a high degree of pollution.
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spelling Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirsEstradiolFollicle-stimulating HormoneTestosterone11-KetotestosteroneVitellogeninThe reproductive physiology of fish can be changed by the presence of pollutants in the water, which act as endocrine disrupting compounds (EDC). We evaluated the impacts of water contaminants in polluted reservoirs acting as possible EDC on the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males. We used biomarkers with different levels of biological organization. Hoplias malabaricus adult males were collected in the summer and winter at five different sites in the Tiete River Basin: the Ponte Nova reservoir (PN), considered a reference site due to the low anthropogenic influence; the Billings reservoir (BIL) at two different branches; and the Guarapiranga reservoir (GUA) at two different branches. Astyanax fasciatus adult males were collected at PN and BIL. BIL and GUA are subjected to great anthropogenic action. We analyzed gonadal histomorphology, testosterone (T), 11-ketotestosterone (11-KT), estradiol (E2) plasma levels, and gene expression of hepatic vitellogenin (vtgA) and pituitary follicle stimulating hormone (fsh beta). In the PN reservoir (reference), the biomarkers analyzed in both species did not differ between the periods analyzed. This is an evidence that the animals keep the same reproductive activity during both seasons. The changes in the plasma concentration of gonadal steroids in both species in polluted reservoirs suggest the presence of EDC compounds in the water and/ or adjusts of the physiological setpoint to allow the reproduction in such adverse conditions. The use of vtgA as biomarker suggests the presence of estrogenic compounds, mainly in BIL, but with a more evident response of H. malabaricus. However, even considering physiological changes, both species present testes during the maturation phase that allow the reproduction in an environment with a high degree of pollution.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Lab Metab & Reprod Organismas Aquat, Rua Matao,Trav 14,321, BR-05508090 Sao Paulo, SP, BrazilUniv Anhembi Morumbi, Escola Ciencias Saude, R Dr Almeida Lima,1134,Parque Mooca, BR-03164000 Sao Paulo, SP, BrazilUniv Estadual Paulista, Ctr Aquicultura CAUNESP, V Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilUniv Estadual Paulista, Ctr Aquicultura CAUNESP, V Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2014/16320-7FAPESP: 2017/11530-1CNPq: 133759/2017-2CAPES: 001Elsevier B.V.Universidade de São Paulo (USP)Univ Anhembi MorumbiUniversidade Estadual Paulista (Unesp)Escalante-Rojas, Maria C.Tolussi, Carlos E.Gomes, Aline D.Munoz-Penuela, MarcelaBrambila-Souza, Gabriela [UNESP]Branco, Giovana S. [UNESP]Moreira, Renata G.2021-06-25T12:30:55Z2021-06-25T12:30:55Z2021-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.ecoenv.2020.111502Ecotoxicology And Environmental Safety. San Diego: Academic Press Inc Elsevier Science, v. 208, 11 p., 2021.0147-6513http://hdl.handle.net/11449/20983710.1016/j.ecoenv.2020.111502WOS:000604137200004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology And Environmental Safetyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:53Zoai:repositorio.unesp.br:11449/209837Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:18:26.254328Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
title Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
spellingShingle Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
Escalante-Rojas, Maria C.
Estradiol
Follicle-stimulating Hormone
Testosterone
11-Ketotestosterone
Vitellogenin
title_short Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
title_full Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
title_fullStr Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
title_full_unstemmed Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
title_sort Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs
author Escalante-Rojas, Maria C.
author_facet Escalante-Rojas, Maria C.
Tolussi, Carlos E.
Gomes, Aline D.
Munoz-Penuela, Marcela
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Moreira, Renata G.
author_role author
author2 Tolussi, Carlos E.
Gomes, Aline D.
Munoz-Penuela, Marcela
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Moreira, Renata G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Univ Anhembi Morumbi
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Escalante-Rojas, Maria C.
Tolussi, Carlos E.
Gomes, Aline D.
Munoz-Penuela, Marcela
Brambila-Souza, Gabriela [UNESP]
Branco, Giovana S. [UNESP]
Moreira, Renata G.
dc.subject.por.fl_str_mv Estradiol
Follicle-stimulating Hormone
Testosterone
11-Ketotestosterone
Vitellogenin
topic Estradiol
Follicle-stimulating Hormone
Testosterone
11-Ketotestosterone
Vitellogenin
description The reproductive physiology of fish can be changed by the presence of pollutants in the water, which act as endocrine disrupting compounds (EDC). We evaluated the impacts of water contaminants in polluted reservoirs acting as possible EDC on the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males. We used biomarkers with different levels of biological organization. Hoplias malabaricus adult males were collected in the summer and winter at five different sites in the Tiete River Basin: the Ponte Nova reservoir (PN), considered a reference site due to the low anthropogenic influence; the Billings reservoir (BIL) at two different branches; and the Guarapiranga reservoir (GUA) at two different branches. Astyanax fasciatus adult males were collected at PN and BIL. BIL and GUA are subjected to great anthropogenic action. We analyzed gonadal histomorphology, testosterone (T), 11-ketotestosterone (11-KT), estradiol (E2) plasma levels, and gene expression of hepatic vitellogenin (vtgA) and pituitary follicle stimulating hormone (fsh beta). In the PN reservoir (reference), the biomarkers analyzed in both species did not differ between the periods analyzed. This is an evidence that the animals keep the same reproductive activity during both seasons. The changes in the plasma concentration of gonadal steroids in both species in polluted reservoirs suggest the presence of EDC compounds in the water and/ or adjusts of the physiological setpoint to allow the reproduction in such adverse conditions. The use of vtgA as biomarker suggests the presence of estrogenic compounds, mainly in BIL, but with a more evident response of H. malabaricus. However, even considering physiological changes, both species present testes during the maturation phase that allow the reproduction in an environment with a high degree of pollution.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:30:55Z
2021-06-25T12:30:55Z
2021-01-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ecoenv.2020.111502
Ecotoxicology And Environmental Safety. San Diego: Academic Press Inc Elsevier Science, v. 208, 11 p., 2021.
0147-6513
http://hdl.handle.net/11449/209837
10.1016/j.ecoenv.2020.111502
WOS:000604137200004
url http://dx.doi.org/10.1016/j.ecoenv.2020.111502
http://hdl.handle.net/11449/209837
identifier_str_mv Ecotoxicology And Environmental Safety. San Diego: Academic Press Inc Elsevier Science, v. 208, 11 p., 2021.
0147-6513
10.1016/j.ecoenv.2020.111502
WOS:000604137200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ecotoxicology And Environmental Safety
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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