Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016001200003 http://hdl.handle.net/11449/174308 |
Resumo: | In addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150 CoPS were identified respectively: 105 (70%) S. aureus, 21(14%), S. hyicus, 19(13%) S. intermedius e 5(3%) S. schleiferi subsp coagulans. Considering the 150 CoNS isolates, the identifications performed by phenotypic and genotypic tests presented 96.7% of concordance, kappa coefficient of agreement = 0.933, SE (standard error) of kappa=0.021 (95% confidence interval: 0.893 to 0.974), Pearson's correlation coefficient (r) = 0.9977, (confidence interval 95%: 0.9938 a 0.9992) and in relation to 150 CPS isolates it was detected an agreement of 98.7%, kappa = 0.960, SE of kappa = 0.016, (95% confidence interval: 0.929 to 0.992) Pearson's correlation coefficient (r) = 0.9994 (95% confidence interval: 0.9681 to 1.0000). The verified agreement strength between the identification methods can be considered as excellent. These results assure that according to laboratory resources any of them will be suitable to perform the staphylococci identification. |
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Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitisBiochemical testsCoagulase negative StaphylococcusCoagulase positive StaphylococcusGenotypeMastitisPhenotypeRpoB geneSequencingStaphylococcus spp.In addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150 CoPS were identified respectively: 105 (70%) S. aureus, 21(14%), S. hyicus, 19(13%) S. intermedius e 5(3%) S. schleiferi subsp coagulans. Considering the 150 CoNS isolates, the identifications performed by phenotypic and genotypic tests presented 96.7% of concordance, kappa coefficient of agreement = 0.933, SE (standard error) of kappa=0.021 (95% confidence interval: 0.893 to 0.974), Pearson's correlation coefficient (r) = 0.9977, (confidence interval 95%: 0.9938 a 0.9992) and in relation to 150 CPS isolates it was detected an agreement of 98.7%, kappa = 0.960, SE of kappa = 0.016, (95% confidence interval: 0.929 to 0.992) Pearson's correlation coefficient (r) = 0.9994 (95% confidence interval: 0.9681 to 1.0000). The verified agreement strength between the identification methods can be considered as excellent. These results assure that according to laboratory resources any of them will be suitable to perform the staphylococci identification.Research on Mastitis (NUPEMAS) Departamento de Higiene Veterinaria e Saude Publica Faculdade de Medicna Veterinária e Zootecnia (FMVZ) Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Distrito de Rubião Jr s/nResearch on Mammary Gland and Milk Production (NAPGAMA) Departamento de Medicina Veterinária Preventiva Universidade de São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva 87Research on Mastitis (NUPEMAS) Departamento de Higiene Veterinaria e Saude Publica Faculdade de Medicna Veterinária e Zootecnia (FMVZ) Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Distrito de Rubião Jr s/nUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Guimarães, Felipe Freitas [UNESP]Joaquim, Samea Fernandes [UNESP]Manzi, Marcela Pinho [UNESP]da Silva, Rodrigo Costa [UNESP]de Oliveira Bruder-Nascimento, Ariane Cristina Mendes [UNESP]Costa, Elizabeth OliveiraLangoni, Hélio [UNESP]2018-12-11T17:10:22Z2018-12-11T17:10:22Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1160-1164application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016001200003Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1160-1164, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/17430810.1590/S0100-736X2016001200003S0100-736X20160012011602-s2.0-85014747800S0100-736X2016001201160.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-01-02T06:20:02Zoai:repositorio.unesp.br:11449/174308Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-02T06:20:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
title |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
spellingShingle |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis Guimarães, Felipe Freitas [UNESP] Biochemical tests Coagulase negative Staphylococcus Coagulase positive Staphylococcus Genotype Mastitis Phenotype RpoB gene Sequencing Staphylococcus spp. |
title_short |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
title_full |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
title_fullStr |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
title_full_unstemmed |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
title_sort |
Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis |
author |
Guimarães, Felipe Freitas [UNESP] |
author_facet |
Guimarães, Felipe Freitas [UNESP] Joaquim, Samea Fernandes [UNESP] Manzi, Marcela Pinho [UNESP] da Silva, Rodrigo Costa [UNESP] de Oliveira Bruder-Nascimento, Ariane Cristina Mendes [UNESP] Costa, Elizabeth Oliveira Langoni, Hélio [UNESP] |
author_role |
author |
author2 |
Joaquim, Samea Fernandes [UNESP] Manzi, Marcela Pinho [UNESP] da Silva, Rodrigo Costa [UNESP] de Oliveira Bruder-Nascimento, Ariane Cristina Mendes [UNESP] Costa, Elizabeth Oliveira Langoni, Hélio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Guimarães, Felipe Freitas [UNESP] Joaquim, Samea Fernandes [UNESP] Manzi, Marcela Pinho [UNESP] da Silva, Rodrigo Costa [UNESP] de Oliveira Bruder-Nascimento, Ariane Cristina Mendes [UNESP] Costa, Elizabeth Oliveira Langoni, Hélio [UNESP] |
dc.subject.por.fl_str_mv |
Biochemical tests Coagulase negative Staphylococcus Coagulase positive Staphylococcus Genotype Mastitis Phenotype RpoB gene Sequencing Staphylococcus spp. |
topic |
Biochemical tests Coagulase negative Staphylococcus Coagulase positive Staphylococcus Genotype Mastitis Phenotype RpoB gene Sequencing Staphylococcus spp. |
description |
In addition to Staphylococcus aureus nowadays other coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS), earlier considered of minor importance, are now accepted as relevant pathogens for humans and animals. The involvement of these microorganisms in bovine mastitis etiology and the possibility their transmission through milk to humans justify the requirement of developing reliable methods for identification of the most frequent species among them. The purpose of this study was to compare the phenotypic techniques with the genotypic method carried out by sequencing of the rpoB gene in identification of several species of the genus Staphylococcus isolated from bovine mastitis. A total of 300 staphylococci isolates of bovine mastitis cases from several Brazilian dairy herds were studied by phenotypic and genotypic techniques, respectively: 150 CoPS and 150 CoNS strains. A total of 18 CoNS different species and 4 CoPS species were identified. Among the CoNS the following species were recognized: 48 (32%) Staphylococcus warneri, 22(15%) S. epidermidis, 20(13%) S. hyicus, 10(7%) S. xylosus, 7(5%) S. haemolyticus, 6(4%) S. simulans, 6(4%) S. schleiferi subsp schleiferi, 6(4%) S. hominis, 5(3%) S. pasteuri, 4(2.7%) S. cohnii, 3(2%) S. saprophyticus subsp. saprophyticus 3(2%) S. chromogenes 3(2%) S. sciuri, 2(1%) S. saccharolyticus, 2(1%) S. lugdunensi, 1(0,7%) S. auricularis, 1(70%) S. saprophyticus subsp. bovis, 1(0.7%) S. capitis. And among the 150 CoPS were identified respectively: 105 (70%) S. aureus, 21(14%), S. hyicus, 19(13%) S. intermedius e 5(3%) S. schleiferi subsp coagulans. Considering the 150 CoNS isolates, the identifications performed by phenotypic and genotypic tests presented 96.7% of concordance, kappa coefficient of agreement = 0.933, SE (standard error) of kappa=0.021 (95% confidence interval: 0.893 to 0.974), Pearson's correlation coefficient (r) = 0.9977, (confidence interval 95%: 0.9938 a 0.9992) and in relation to 150 CPS isolates it was detected an agreement of 98.7%, kappa = 0.960, SE of kappa = 0.016, (95% confidence interval: 0.929 to 0.992) Pearson's correlation coefficient (r) = 0.9994 (95% confidence interval: 0.9681 to 1.0000). The verified agreement strength between the identification methods can be considered as excellent. These results assure that according to laboratory resources any of them will be suitable to perform the staphylococci identification. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:10:22Z 2018-12-11T17:10:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016001200003 Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1160-1164, 2016. 1678-5150 0100-736X http://hdl.handle.net/11449/174308 10.1590/S0100-736X2016001200003 S0100-736X2016001201160 2-s2.0-85014747800 S0100-736X2016001201160.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016001200003 http://hdl.handle.net/11449/174308 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1160-1164, 2016. 1678-5150 0100-736X 10.1590/S0100-736X2016001200003 S0100-736X2016001201160 2-s2.0-85014747800 S0100-736X2016001201160.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1160-1164 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965489064050688 |