Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin

Detalhes bibliográficos
Autor(a) principal: Takayama, Kátia S.
Data de Publicação: 2022
Outros Autores: Monteiro, Mariana C., Saito, Priscila, Pinto, Ingrid C., Nakano, Claudia T., Martinez, Renata M., Thomaz, Douglas V., Verri, Waldiceu A., Baracat, Marcela M., Arakawa, Nilton S., Russo, Helena M. [UNESP], Zeraik, Maria L., Casagrande, Rubia, Do Couto, Renê O., Georgetti, Sandra Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0001-3765202220201058
http://hdl.handle.net/11449/248003
Resumo: UVB-irradiation increases the risk of various skin disorders, therefore leading to inflammation and oxidative stress. In this sense, antioxidant-rich herbs such as Rosmarinus officinalis may be useful in minimizing the damage promoted by reactive oxygen species. In this work, we report the efficacy of a R. officinalis hydroethanolic extract (ROe)-loaded emulgel in preventing UVB-related skin damage. Total phenols were determined using Folin-Ciocalteu assay, and the main phytocomponents in the extract were identified by UHPLC-HRMS. Moreover, in vitro sun protection factor (SPF) value of ROe was also assessed, and we investigated the in vivo protective effect of an emulgel containing ROe against UVB-induced damage in an animal model. The ROe exhibited commercially viable SPF activity (7.56 ± 0.16) and remarkable polyphenolic content (24.15 ± 0.11 mg (Eq.GA)/g). HPLC-MS and UHPLC-HRMS results showcased that the main compounds in ROe were: rosmarinic acid, carnosic acid and carnosol. The evaluation of the in vitro antioxidant activity demonstrated a dose-dependent effect of ROe against several radicals and the capacity to reduce iron. Therefore, we demonstrated that topical application of the formulation containing ROe inhibited edema formation, myeloperoxidase activity, GSH depletion and maintained ferric reducing (FRAP) and ABTS scavenging abilities of the skin after UVB exposure.
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spelling Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skinAntioxidantscosmeceuticalsdermatologyphytochemicalssunscreensUVB-irradiation increases the risk of various skin disorders, therefore leading to inflammation and oxidative stress. In this sense, antioxidant-rich herbs such as Rosmarinus officinalis may be useful in minimizing the damage promoted by reactive oxygen species. In this work, we report the efficacy of a R. officinalis hydroethanolic extract (ROe)-loaded emulgel in preventing UVB-related skin damage. Total phenols were determined using Folin-Ciocalteu assay, and the main phytocomponents in the extract were identified by UHPLC-HRMS. Moreover, in vitro sun protection factor (SPF) value of ROe was also assessed, and we investigated the in vivo protective effect of an emulgel containing ROe against UVB-induced damage in an animal model. The ROe exhibited commercially viable SPF activity (7.56 ± 0.16) and remarkable polyphenolic content (24.15 ± 0.11 mg (Eq.GA)/g). HPLC-MS and UHPLC-HRMS results showcased that the main compounds in ROe were: rosmarinic acid, carnosic acid and carnosol. The evaluation of the in vitro antioxidant activity demonstrated a dose-dependent effect of ROe against several radicals and the capacity to reduce iron. Therefore, we demonstrated that topical application of the formulation containing ROe inhibited edema formation, myeloperoxidase activity, GSH depletion and maintained ferric reducing (FRAP) and ABTS scavenging abilities of the skin after UVB exposure.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Londrina-UEL Departamento de Ciências Farmacêuticas, Avenida Robert Koch, 60, Hospital Universitário, PRUniversidade Federal de Goiás Faculdade de Farmácia, Rua 240, s/n, Setor Leste Universitário, GOUniversidade Estadual de Londrina-UEL Departamento de Patologia, Rodovia Celso Garcia Cid, Km 380, PR 445, Caixa Postal 10011, PRUniversidade Estadual Paulista-UNESP Instituto de Química Núcleos de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais-NuBBE Departamento de Química Orgânica, Avenida Prof. Francisco Degni, 55, SPUniversidade Estadual de Londrina – UEL Laboratório de Fitoquímica e Biomoléculas-LabFitoBio Departamento de Química, Rodovia Celso Garcia Cid, Km 380, PRUniversidade Federal de São João del-Rei Laboratório de Desenvolvimento Farmacotécnico-LADEF, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho, MGUniversidade Estadual Paulista-UNESP Instituto de Química Núcleos de Bioensaios Biossíntese e Ecofisiologia de Produtos Naturais-NuBBE Departamento de Química Orgânica, Avenida Prof. Francisco Degni, 55, SPCAPES: 001CNPq: 307186/2017-2CNPq: 307852/2019-9Universidade Estadual de Londrina (UEL)Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP)Laboratório de Desenvolvimento Farmacotécnico-LADEFTakayama, Kátia S.Monteiro, Mariana C.Saito, PriscilaPinto, Ingrid C.Nakano, Claudia T.Martinez, Renata M.Thomaz, Douglas V.Verri, Waldiceu A.Baracat, Marcela M.Arakawa, Nilton S.Russo, Helena M. [UNESP]Zeraik, Maria L.Casagrande, RubiaDo Couto, Renê O.Georgetti, Sandra Regina2023-07-29T13:31:44Z2023-07-29T13:31:44Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/0001-3765202220201058Anais da Academia Brasileira de Ciencias, v. 94, n. 4, 2022.1678-26900001-3765http://hdl.handle.net/11449/24800310.1590/0001-37652022202010582-s2.0-85143569527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais da Academia Brasileira de Cienciasinfo:eu-repo/semantics/openAccess2023-07-29T13:31:44Zoai:repositorio.unesp.br:11449/248003Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:34:06.500914Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
title Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
spellingShingle Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
Takayama, Kátia S.
Antioxidants
cosmeceuticals
dermatology
phytochemicals
sunscreens
title_short Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
title_full Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
title_fullStr Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
title_full_unstemmed Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
title_sort Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
author Takayama, Kátia S.
author_facet Takayama, Kátia S.
Monteiro, Mariana C.
Saito, Priscila
Pinto, Ingrid C.
Nakano, Claudia T.
Martinez, Renata M.
Thomaz, Douglas V.
Verri, Waldiceu A.
Baracat, Marcela M.
Arakawa, Nilton S.
Russo, Helena M. [UNESP]
Zeraik, Maria L.
Casagrande, Rubia
Do Couto, Renê O.
Georgetti, Sandra Regina
author_role author
author2 Monteiro, Mariana C.
Saito, Priscila
Pinto, Ingrid C.
Nakano, Claudia T.
Martinez, Renata M.
Thomaz, Douglas V.
Verri, Waldiceu A.
Baracat, Marcela M.
Arakawa, Nilton S.
Russo, Helena M. [UNESP]
Zeraik, Maria L.
Casagrande, Rubia
Do Couto, Renê O.
Georgetti, Sandra Regina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (UNESP)
Laboratório de Desenvolvimento Farmacotécnico-LADEF
dc.contributor.author.fl_str_mv Takayama, Kátia S.
Monteiro, Mariana C.
Saito, Priscila
Pinto, Ingrid C.
Nakano, Claudia T.
Martinez, Renata M.
Thomaz, Douglas V.
Verri, Waldiceu A.
Baracat, Marcela M.
Arakawa, Nilton S.
Russo, Helena M. [UNESP]
Zeraik, Maria L.
Casagrande, Rubia
Do Couto, Renê O.
Georgetti, Sandra Regina
dc.subject.por.fl_str_mv Antioxidants
cosmeceuticals
dermatology
phytochemicals
sunscreens
topic Antioxidants
cosmeceuticals
dermatology
phytochemicals
sunscreens
description UVB-irradiation increases the risk of various skin disorders, therefore leading to inflammation and oxidative stress. In this sense, antioxidant-rich herbs such as Rosmarinus officinalis may be useful in minimizing the damage promoted by reactive oxygen species. In this work, we report the efficacy of a R. officinalis hydroethanolic extract (ROe)-loaded emulgel in preventing UVB-related skin damage. Total phenols were determined using Folin-Ciocalteu assay, and the main phytocomponents in the extract were identified by UHPLC-HRMS. Moreover, in vitro sun protection factor (SPF) value of ROe was also assessed, and we investigated the in vivo protective effect of an emulgel containing ROe against UVB-induced damage in an animal model. The ROe exhibited commercially viable SPF activity (7.56 ± 0.16) and remarkable polyphenolic content (24.15 ± 0.11 mg (Eq.GA)/g). HPLC-MS and UHPLC-HRMS results showcased that the main compounds in ROe were: rosmarinic acid, carnosic acid and carnosol. The evaluation of the in vitro antioxidant activity demonstrated a dose-dependent effect of ROe against several radicals and the capacity to reduce iron. Therefore, we demonstrated that topical application of the formulation containing ROe inhibited edema formation, myeloperoxidase activity, GSH depletion and maintained ferric reducing (FRAP) and ABTS scavenging abilities of the skin after UVB exposure.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T13:31:44Z
2023-07-29T13:31:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0001-3765202220201058
Anais da Academia Brasileira de Ciencias, v. 94, n. 4, 2022.
1678-2690
0001-3765
http://hdl.handle.net/11449/248003
10.1590/0001-3765202220201058
2-s2.0-85143569527
url http://dx.doi.org/10.1590/0001-3765202220201058
http://hdl.handle.net/11449/248003
identifier_str_mv Anais da Academia Brasileira de Ciencias, v. 94, n. 4, 2022.
1678-2690
0001-3765
10.1590/0001-3765202220201058
2-s2.0-85143569527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anais da Academia Brasileira de Ciencias
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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