What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12865-020-00367-8 http://hdl.handle.net/11449/197014 |
Resumo: | Background Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA. |
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What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA?CytokinesDental pulpFibroblasts and lipopolysaccharideBackground Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru, SP, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP, BrazilJackson Lab, 600 Main St, Bar Harbor, ME 04609 USASao Paulo State Univ, Sch Dent Aracatuba, Dept Basic Sci, Aracatuba, SP, BrazilFAPESP: 2015/03965-2FAPESP: 2016/11450-5CNPq: 307986/2017-9CAPES: 001BmcUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Jackson LabColombini-Ishikiriama, Bella LunaDionisio, Thiago JoseGarbieri, Thais FranciniSilva, Rafaela Alves daAndrade Moreira Machado, Maria AparecidaPenha de Oliveira, Sandra Helena [UNESP]Lara, Vanessa SoaresGreene, Andrew SethSantos, Carlos Ferreira2020-12-10T20:03:31Z2020-12-10T20:03:31Z2020-06-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1186/s12865-020-00367-8Bmc Immunology. London: Bmc, v. 21, n. 1, 9 p., 2020.http://hdl.handle.net/11449/19701410.1186/s12865-020-00367-8WOS:000543399500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Immunologyinfo:eu-repo/semantics/openAccess2024-09-19T14:03:15Zoai:repositorio.unesp.br:11449/197014Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:03:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
title |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
spellingShingle |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? Colombini-Ishikiriama, Bella Luna Cytokines Dental pulp Fibroblasts and lipopolysaccharide |
title_short |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
title_full |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
title_fullStr |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
title_full_unstemmed |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
title_sort |
What is the response profile of deciduous pulp fibroblasts stimulated withE. coliLPS andE. faecalisLTA? |
author |
Colombini-Ishikiriama, Bella Luna |
author_facet |
Colombini-Ishikiriama, Bella Luna Dionisio, Thiago Jose Garbieri, Thais Francini Silva, Rafaela Alves da Andrade Moreira Machado, Maria Aparecida Penha de Oliveira, Sandra Helena [UNESP] Lara, Vanessa Soares Greene, Andrew Seth Santos, Carlos Ferreira |
author_role |
author |
author2 |
Dionisio, Thiago Jose Garbieri, Thais Francini Silva, Rafaela Alves da Andrade Moreira Machado, Maria Aparecida Penha de Oliveira, Sandra Helena [UNESP] Lara, Vanessa Soares Greene, Andrew Seth Santos, Carlos Ferreira |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Jackson Lab |
dc.contributor.author.fl_str_mv |
Colombini-Ishikiriama, Bella Luna Dionisio, Thiago Jose Garbieri, Thais Francini Silva, Rafaela Alves da Andrade Moreira Machado, Maria Aparecida Penha de Oliveira, Sandra Helena [UNESP] Lara, Vanessa Soares Greene, Andrew Seth Santos, Carlos Ferreira |
dc.subject.por.fl_str_mv |
Cytokines Dental pulp Fibroblasts and lipopolysaccharide |
topic |
Cytokines Dental pulp Fibroblasts and lipopolysaccharide |
description |
Background Oral fibroblast immunological responses to bacterial stimuli are well known. However, there are few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which are important for the treatment of pulp infections in children. The aim of this study was to evaluate expression and production of inflammatory cytokines and chemokines by HDPF when challenged with bacterial antigens normally present in advanced caries lesions. Methods Triplicate HDPF from 4 children (n = 4; 2 boys and 2 girls) were cultured by explant technique and challenged or not withEscherichia colilipopolysaccharide/1 mu g/mL(EcLPS)orEnterococcus faecalislipoteichoic acid/1 mu g/mL(EfLTA)for 6 and 24 h. Most of published studies employed immortalized cells, i.e., without checking possible gender and genetic variables. mRNA expression and protein production were evaluated by RT-qPCR and ELISA MILLIPLEX (R), respectively, for Interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, Chemokine C-C motif ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1), Chemokine C-C motif ligand 3/macrophage inflammatory protein 1-alpha (CCL3/MIP1-alpha), Chemokine C-C motif ligand 5/ regulated on activation, normal T cell expressed and secreted (CCL5/RANTES), C-X-C motif chemokine 12/ stromal cell-derived factor 1 (CXCL12/SDF-1), Tumor Necrosis Factor-alpha (TNF-alpha), Interferon-gamma (IFN gamma), Vascular Endothelial Growth Factor (VEGF), Colony stimulating factor 1 (CSF-1) and Macrophage colony-stimulating factor (M-CSF). Results EcLPSincreased IL-1 alpha, IL-1 beta, IL-8, CCL2, CCL5, TNF-alpha and CSF-1 mRNA and protein levels whileEfLTAwas only able to positively regulate gene expression and protein production of IL-8. Conclusion The results of the present study confirmed our hypothesis, since pulp fibroblasts from deciduous teeth are capable of increasing gene expression and protein production after being stimulated withEcLPSandEfLTA. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:03:31Z 2020-12-10T20:03:31Z 2020-06-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12865-020-00367-8 Bmc Immunology. London: Bmc, v. 21, n. 1, 9 p., 2020. http://hdl.handle.net/11449/197014 10.1186/s12865-020-00367-8 WOS:000543399500001 |
url |
http://dx.doi.org/10.1186/s12865-020-00367-8 http://hdl.handle.net/11449/197014 |
identifier_str_mv |
Bmc Immunology. London: Bmc, v. 21, n. 1, 9 p., 2020. 10.1186/s12865-020-00367-8 WOS:000543399500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Bmc |
publisher.none.fl_str_mv |
Bmc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546528480952320 |