Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2019/5805609 http://hdl.handle.net/11449/187858 |
Resumo: | Here we described an aptasensor based on graphene oxide (GO) decorated with gold nanoparticles (AuNPs) able to detect the AR released by prostate cells, in order to provide new insights about the relationship between AR and prostate cancer for a more precise diagnosis for cancer. Characterization of the surface modified GE was carried out by cyclic voltammetry and electrochemical impedance spectroscopy using ferrocene as a redox probe. Under the optimized experimental conditions, a detection limit of 0.5 ng/mL (3σ/slope) was obtained by the present electrochemical biosensor, along with a linear range of 0-110 ng/mL. By virtue of excellent sensitivity, specificity, and repeatability, the present electrochemical biosensor provides a potential application in clinical diagnosis. |
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Repositório Institucional da UNESP |
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spelling |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cellsHere we described an aptasensor based on graphene oxide (GO) decorated with gold nanoparticles (AuNPs) able to detect the AR released by prostate cells, in order to provide new insights about the relationship between AR and prostate cancer for a more precise diagnosis for cancer. Characterization of the surface modified GE was carried out by cyclic voltammetry and electrochemical impedance spectroscopy using ferrocene as a redox probe. Under the optimized experimental conditions, a detection limit of 0.5 ng/mL (3σ/slope) was obtained by the present electrochemical biosensor, along with a linear range of 0-110 ng/mL. By virtue of excellent sensitivity, specificity, and repeatability, the present electrochemical biosensor provides a potential application in clinical diagnosis.Institute of Bioscience Department of Chemistry and Biochemistry UNESPInstitute of Bioscience Department of Chemistry and Biochemistry UNESPUniversidade Estadual Paulista (Unesp)Crulhas, Bruno P. [UNESP]Basso, Caroline R. [UNESP]Parra, Joaõ P. R. L. L. [UNESP]Castro, Gustavo R. [UNESP]Pedrosa, Valber A. [UNESP]2019-10-06T15:49:28Z2019-10-06T15:49:28Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2019/5805609Journal of Sensors, v. 2019.1687-72681687-725Xhttp://hdl.handle.net/11449/18785810.1155/2019/58056092-s2.0-85068881898Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Sensorsinfo:eu-repo/semantics/openAccess2021-10-22T19:44:38Zoai:repositorio.unesp.br:11449/187858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:44:17.496174Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
title |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
spellingShingle |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells Crulhas, Bruno P. [UNESP] |
title_short |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
title_full |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
title_fullStr |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
title_full_unstemmed |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
title_sort |
Reduced graphene oxide decorated with AuNPs as a new aptamer-based biosensor for the detection of androgen receptor from prostate cells |
author |
Crulhas, Bruno P. [UNESP] |
author_facet |
Crulhas, Bruno P. [UNESP] Basso, Caroline R. [UNESP] Parra, Joaõ P. R. L. L. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author_role |
author |
author2 |
Basso, Caroline R. [UNESP] Parra, Joaõ P. R. L. L. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Crulhas, Bruno P. [UNESP] Basso, Caroline R. [UNESP] Parra, Joaõ P. R. L. L. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
description |
Here we described an aptasensor based on graphene oxide (GO) decorated with gold nanoparticles (AuNPs) able to detect the AR released by prostate cells, in order to provide new insights about the relationship between AR and prostate cancer for a more precise diagnosis for cancer. Characterization of the surface modified GE was carried out by cyclic voltammetry and electrochemical impedance spectroscopy using ferrocene as a redox probe. Under the optimized experimental conditions, a detection limit of 0.5 ng/mL (3σ/slope) was obtained by the present electrochemical biosensor, along with a linear range of 0-110 ng/mL. By virtue of excellent sensitivity, specificity, and repeatability, the present electrochemical biosensor provides a potential application in clinical diagnosis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:49:28Z 2019-10-06T15:49:28Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2019/5805609 Journal of Sensors, v. 2019. 1687-7268 1687-725X http://hdl.handle.net/11449/187858 10.1155/2019/5805609 2-s2.0-85068881898 |
url |
http://dx.doi.org/10.1155/2019/5805609 http://hdl.handle.net/11449/187858 |
identifier_str_mv |
Journal of Sensors, v. 2019. 1687-7268 1687-725X 10.1155/2019/5805609 2-s2.0-85068881898 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Sensors |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128410630750208 |