Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10008-016-3182-y http://hdl.handle.net/11449/159048 |
Resumo: | In this work, highly sensitive and selective hydrogel microstructures to detect hydrogen peroxide releasing from cancer cell based on electrochemical biosensors are proposed. Gold nanoparticles (AuNPs) were conjugated with horseradish peroxidase and were dispersed in the prepolymer solution of poly(ethylene glycol) diacrylate. The prepolymer solution was photolithographically patterned in alignment with an array of Au microelectrodes fabricated on glass. Performance of this biosensor was characterized by transmission electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Under the optimal condition, the proposed biosensor can detect hydrogen peroxide (H2O2) in a wide linear range from 2 to 100 mu M with a low detection limit of 0.01 mu M. It can be also directly used to mark out extracellular H2O2 released from prostate cells. Furthermore, the reproducibility, stability, and selectivity of the biosensor are analogous with the previous report, so this methodology can be used in physiological and pathological detection of H2O2 in the future. |
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Repositório Institucional da UNESP |
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Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensorIn this work, highly sensitive and selective hydrogel microstructures to detect hydrogen peroxide releasing from cancer cell based on electrochemical biosensors are proposed. Gold nanoparticles (AuNPs) were conjugated with horseradish peroxidase and were dispersed in the prepolymer solution of poly(ethylene glycol) diacrylate. The prepolymer solution was photolithographically patterned in alignment with an array of Au microelectrodes fabricated on glass. Performance of this biosensor was characterized by transmission electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Under the optimal condition, the proposed biosensor can detect hydrogen peroxide (H2O2) in a wide linear range from 2 to 100 mu M with a low detection limit of 0.01 mu M. It can be also directly used to mark out extracellular H2O2 released from prostate cells. Furthermore, the reproducibility, stability, and selectivity of the biosensor are analogous with the previous report, so this methodology can be used in physiological and pathological detection of H2O2 in the future.Univ Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilSpringerUniversidade Estadual Paulista (Unesp)Crulhas, Bruno P. [UNESP]Ramos, Naira P. [UNESP]Castro, Gustavo R. [UNESP]Pedrosa, Valber A. [UNESP]2018-11-26T15:31:05Z2018-11-26T15:31:05Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2427-2433application/pdfhttp://dx.doi.org/10.1007/s10008-016-3182-yJournal Of Solid State Electrochemistry. New York: Springer, v. 20, n. 9, p. 2427-2433, 2016.1432-8488http://hdl.handle.net/11449/15904810.1007/s10008-016-3182-yWOS:000382130000007WOS000382130000007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Solid State Electrochemistry0,661info:eu-repo/semantics/openAccess2024-01-11T06:25:29Zoai:repositorio.unesp.br:11449/159048Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:07.717797Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
title |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
spellingShingle |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor Crulhas, Bruno P. [UNESP] |
title_short |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
title_full |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
title_fullStr |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
title_full_unstemmed |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
title_sort |
Detection of hydrogen peroxide releasing from prostate cancer cell using a biosensor |
author |
Crulhas, Bruno P. [UNESP] |
author_facet |
Crulhas, Bruno P. [UNESP] Ramos, Naira P. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author_role |
author |
author2 |
Ramos, Naira P. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Crulhas, Bruno P. [UNESP] Ramos, Naira P. [UNESP] Castro, Gustavo R. [UNESP] Pedrosa, Valber A. [UNESP] |
description |
In this work, highly sensitive and selective hydrogel microstructures to detect hydrogen peroxide releasing from cancer cell based on electrochemical biosensors are proposed. Gold nanoparticles (AuNPs) were conjugated with horseradish peroxidase and were dispersed in the prepolymer solution of poly(ethylene glycol) diacrylate. The prepolymer solution was photolithographically patterned in alignment with an array of Au microelectrodes fabricated on glass. Performance of this biosensor was characterized by transmission electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. Under the optimal condition, the proposed biosensor can detect hydrogen peroxide (H2O2) in a wide linear range from 2 to 100 mu M with a low detection limit of 0.01 mu M. It can be also directly used to mark out extracellular H2O2 released from prostate cells. Furthermore, the reproducibility, stability, and selectivity of the biosensor are analogous with the previous report, so this methodology can be used in physiological and pathological detection of H2O2 in the future. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-11-26T15:31:05Z 2018-11-26T15:31:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10008-016-3182-y Journal Of Solid State Electrochemistry. New York: Springer, v. 20, n. 9, p. 2427-2433, 2016. 1432-8488 http://hdl.handle.net/11449/159048 10.1007/s10008-016-3182-y WOS:000382130000007 WOS000382130000007.pdf |
url |
http://dx.doi.org/10.1007/s10008-016-3182-y http://hdl.handle.net/11449/159048 |
identifier_str_mv |
Journal Of Solid State Electrochemistry. New York: Springer, v. 20, n. 9, p. 2427-2433, 2016. 1432-8488 10.1007/s10008-016-3182-y WOS:000382130000007 WOS000382130000007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Solid State Electrochemistry 0,661 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2427-2433 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129448646541312 |