Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2017.04.031 http://hdl.handle.net/11449/174507 |
Resumo: | To test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations. |
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Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic maresEmbryo transferEquineHormonal protocolsSteroid receptorsTo test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal ScienceFAPESP: 2012/00298-7Universidade Estadual Paulista (Unesp)Silva, E. S.M. [UNESP]Ignácio, F. S. [UNESP]Fritsch, S. C. [UNESP]Zanoni, D. S. [UNESP]Pantoja, J. C.F. [UNESP]Oliveira-Filho, J. P. [UNESP]Meira, C. [UNESP]2018-12-11T17:11:28Z2018-12-11T17:11:28Z2017-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article159-169application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2017.04.031Theriogenology, v. 97, p. 159-169.0093-691Xhttp://hdl.handle.net/11449/17450710.1016/j.theriogenology.2017.04.0312-s2.0-850184195172-s2.0-85018419517.pdf00429054157111990000-0002-2245-800XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:00:56Zoai:repositorio.unesp.br:11449/174507Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
title |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
spellingShingle |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares Silva, E. S.M. [UNESP] Embryo transfer Equine Hormonal protocols Steroid receptors |
title_short |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
title_full |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
title_fullStr |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
title_full_unstemmed |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
title_sort |
Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares |
author |
Silva, E. S.M. [UNESP] |
author_facet |
Silva, E. S.M. [UNESP] Ignácio, F. S. [UNESP] Fritsch, S. C. [UNESP] Zanoni, D. S. [UNESP] Pantoja, J. C.F. [UNESP] Oliveira-Filho, J. P. [UNESP] Meira, C. [UNESP] |
author_role |
author |
author2 |
Ignácio, F. S. [UNESP] Fritsch, S. C. [UNESP] Zanoni, D. S. [UNESP] Pantoja, J. C.F. [UNESP] Oliveira-Filho, J. P. [UNESP] Meira, C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, E. S.M. [UNESP] Ignácio, F. S. [UNESP] Fritsch, S. C. [UNESP] Zanoni, D. S. [UNESP] Pantoja, J. C.F. [UNESP] Oliveira-Filho, J. P. [UNESP] Meira, C. [UNESP] |
dc.subject.por.fl_str_mv |
Embryo transfer Equine Hormonal protocols Steroid receptors |
topic |
Embryo transfer Equine Hormonal protocols Steroid receptors |
description |
To test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-15 2018-12-11T17:11:28Z 2018-12-11T17:11:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2017.04.031 Theriogenology, v. 97, p. 159-169. 0093-691X http://hdl.handle.net/11449/174507 10.1016/j.theriogenology.2017.04.031 2-s2.0-85018419517 2-s2.0-85018419517.pdf 0042905415711199 0000-0002-2245-800X |
url |
http://dx.doi.org/10.1016/j.theriogenology.2017.04.031 http://hdl.handle.net/11449/174507 |
identifier_str_mv |
Theriogenology, v. 97, p. 159-169. 0093-691X 10.1016/j.theriogenology.2017.04.031 2-s2.0-85018419517 2-s2.0-85018419517.pdf 0042905415711199 0000-0002-2245-800X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
159-169 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546564528898048 |