Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares

Detalhes bibliográficos
Autor(a) principal: Silva, E. S.M. [UNESP]
Data de Publicação: 2017
Outros Autores: Ignácio, F. S. [UNESP], Fritsch, S. C. [UNESP], Zanoni, D. S. [UNESP], Pantoja, J. C.F. [UNESP], Oliveira-Filho, J. P. [UNESP], Meira, C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2017.04.031
http://hdl.handle.net/11449/174507
Resumo: To test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations.
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spelling Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic maresEmbryo transferEquineHormonal protocolsSteroid receptorsTo test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal ScienceUNESP – Univ. Estadual Paulista Campus de Botucatu UNESP – Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal ScienceFAPESP: 2012/00298-7Universidade Estadual Paulista (Unesp)Silva, E. S.M. [UNESP]Ignácio, F. S. [UNESP]Fritsch, S. C. [UNESP]Zanoni, D. S. [UNESP]Pantoja, J. C.F. [UNESP]Oliveira-Filho, J. P. [UNESP]Meira, C. [UNESP]2018-12-11T17:11:28Z2018-12-11T17:11:28Z2017-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article159-169application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2017.04.031Theriogenology, v. 97, p. 159-169.0093-691Xhttp://hdl.handle.net/11449/17450710.1016/j.theriogenology.2017.04.0312-s2.0-850184195172-s2.0-85018419517.pdf00429054157111990000-0002-2245-800XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:00:56Zoai:repositorio.unesp.br:11449/174507Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
title Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
spellingShingle Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
Silva, E. S.M. [UNESP]
Embryo transfer
Equine
Hormonal protocols
Steroid receptors
title_short Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
title_full Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
title_fullStr Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
title_full_unstemmed Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
title_sort Administration of 2.5 mg of estradiol followed by 1,500 mg of progesterone to anovulatory mares promote similar uterine morphology, hormone concentrations and molecular dynamics to those observed in cyclic mares
author Silva, E. S.M. [UNESP]
author_facet Silva, E. S.M. [UNESP]
Ignácio, F. S. [UNESP]
Fritsch, S. C. [UNESP]
Zanoni, D. S. [UNESP]
Pantoja, J. C.F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
author_role author
author2 Ignácio, F. S. [UNESP]
Fritsch, S. C. [UNESP]
Zanoni, D. S. [UNESP]
Pantoja, J. C.F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, E. S.M. [UNESP]
Ignácio, F. S. [UNESP]
Fritsch, S. C. [UNESP]
Zanoni, D. S. [UNESP]
Pantoja, J. C.F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
dc.subject.por.fl_str_mv Embryo transfer
Equine
Hormonal protocols
Steroid receptors
topic Embryo transfer
Equine
Hormonal protocols
Steroid receptors
description To test the hypothesis that the administration of 2.5 mg of estradiol benzoate (EB) followed by 1500 mg of long acting progesterone (LA P4) causes similar uterine changes and molecular dynamics in anovulatory mares to those observed in cyclic ones, we evaluated the changes of estrogen (ERα and ERβ) and progesterone receptors (PR) in anestrous, transitional and cyclic mares by RT-qPCR and immunohistochemistry. In addition, we evaluated uterine edema, tonus and estrogens and progesterone plasma profile. Endometrial biopsies were taken from anestrous and transitional mares immediately before EB injection, 48 h after EB administration and five days after LA P4 was given. In cyclic mares, biopsies were collected at estrus and at five days after ovulation. Similar estrogen peaks were achieved after the injection of the single EB dose between treated and cyclic groups, as well as maximum uterine edema. Uterine tone was increased to diestrus levels after administration of 1500 mg of LA P4. Changes in relative abundance of transcripts for PR, ERα and ERβ when progesterone stimulated endometrium was compared to estrogen stimulated endometrium were similar between cyclic and non-cyclic treated mares. However, apparent decreased PR in the endometrial glandular epithelium was not observed in non-cyclic mares five days after LA P4 administration as observed at five days after ovulation in cyclic mares. The protocol produced similar endometrial edema, uterine tonus and changes in relative abundance of PR, ERα and ERβ transcripts to those observed in cyclic mares during late estrus and early diestrus, as well as similar estradiol and estrogen conjugate plasma concentrations.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-15
2018-12-11T17:11:28Z
2018-12-11T17:11:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2017.04.031
Theriogenology, v. 97, p. 159-169.
0093-691X
http://hdl.handle.net/11449/174507
10.1016/j.theriogenology.2017.04.031
2-s2.0-85018419517
2-s2.0-85018419517.pdf
0042905415711199
0000-0002-2245-800X
url http://dx.doi.org/10.1016/j.theriogenology.2017.04.031
http://hdl.handle.net/11449/174507
identifier_str_mv Theriogenology, v. 97, p. 159-169.
0093-691X
10.1016/j.theriogenology.2017.04.031
2-s2.0-85018419517
2-s2.0-85018419517.pdf
0042905415711199
0000-0002-2245-800X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 159-169
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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