Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold

• Autor(a) principal: Bertanha, Matheus [UNESP]
• Data de Publicação: 2017
• Outros Autores: Sobreira, Marcone Lima [UNESP], Bovolato, Ana Lívia de Carvalho [UNESP], Rinaldi, Jaqueline de Carvalho [UNESP], Reis, Patricia Pintor [UNESP], Moroz, Andrei [UNESP], de Moraes, Leonardo Nazario [UNESP], Deffune, Elenice [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/s0102-865020170090000003; http://hdl.handle.net/11449/175333
• topic: Blood vessels; Peripheral arterial disease; Rabbits; Tissue engineering; Tissue scaffolds

Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1-control, consisting of samples of vena cava in natura; group 2-SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm -Shaker News Brunswick Scientific*) for 1 hour at controlled temperature shaker at 37°C; group 3-SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.