Effect of dietary iron supplementation on the equine fecal microbiome
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Publication Date: | 2023 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://hdl.handle.net/11449/248647 |
Summary: | Iron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at-80°C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies. |
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Effect of dietary iron supplementation on the equine fecal microbiomeIron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at-80°C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies.Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo, São PauloDepartment of Veterinary Clinical Science College of Veterinary Medicine and Animal Science São Paulo State University, São PauloDepartment of Clinical Studies Ontario Veterinary College University of GuelphDepartment of Medicine Farncombe Family Digestive Health Research Institute McMaster UniversityDepartment of Biomedicine Veterinary Medicine Faculty University of MontréalDepartment of Veterinary Clinical Science College of Veterinary Medicine and Animal Science São Paulo State University, São PauloUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)University of GuelphMcMaster UniversityUniversity of MontréalArantes, Julia AssisBorges, Alexandre Secorun [UNESP]Zakia, Luiza StachewskiSurette, Michael GordonWeese, Jeffrey ScottCosta, Marcio CarvalhoArroyo, Luis Guillermo2023-07-29T13:49:45Z2023-07-29T13:49:45Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-104Canadian Journal of Veterinary Research, v. 87, n. 2, p. 97-104, 2023.1928-9022http://hdl.handle.net/11449/2486472-s2.0-85151806935Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Veterinary Researchinfo:eu-repo/semantics/openAccess2023-07-29T13:49:45Zoai:repositorio.unesp.br:11449/248647Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:21.189775Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of dietary iron supplementation on the equine fecal microbiome |
title |
Effect of dietary iron supplementation on the equine fecal microbiome |
spellingShingle |
Effect of dietary iron supplementation on the equine fecal microbiome Arantes, Julia Assis |
title_short |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_full |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_fullStr |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_full_unstemmed |
Effect of dietary iron supplementation on the equine fecal microbiome |
title_sort |
Effect of dietary iron supplementation on the equine fecal microbiome |
author |
Arantes, Julia Assis |
author_facet |
Arantes, Julia Assis Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
author_role |
author |
author2 |
Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) University of Guelph McMaster University University of Montréal |
dc.contributor.author.fl_str_mv |
Arantes, Julia Assis Borges, Alexandre Secorun [UNESP] Zakia, Luiza Stachewski Surette, Michael Gordon Weese, Jeffrey Scott Costa, Marcio Carvalho Arroyo, Luis Guillermo |
description |
Iron is an essential element for all living organisms, including bacteria, as several virulence factors and replication components are influenced by iron concentration. The objective of this study was to determine whether the composition and diversity of the fecal microbiota of adult horses are affected by supplemental dietary iron. Ten clinically healthy horses were randomly divided into a control and an iron-supplemented group (n = 5). The treated group was supplemented with oral ferrous sulphate monohydrate (720 ppm of iron), whereas the control group received 320 ppm of iron daily for 15 d. Fecal samples were collected before and 5, 10, 15, and 30 d after supplementation and frozen at-80°C. DNA was sequenced using an Illumina MiSeq platform and data were analyzed using the software Mothur and linear discriminant analysis (LDA) effect size (LEfSe). Iron supplementation caused no change in the overall composition of the fecal microbiota, but some minor changes were observed in the low-abundant bacteria, as well as an increased alpha diversity after 15 d of supplementation. Significant differences in community composition of the fecal microbiota over time were observed in both groups, highlighting the importance of a control group, as there are variables that cannot be controlled in microbiome studies. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:49:45Z 2023-07-29T13:49:45Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Canadian Journal of Veterinary Research, v. 87, n. 2, p. 97-104, 2023. 1928-9022 http://hdl.handle.net/11449/248647 2-s2.0-85151806935 |
identifier_str_mv |
Canadian Journal of Veterinary Research, v. 87, n. 2, p. 97-104, 2023. 1928-9022 2-s2.0-85151806935 |
url |
http://hdl.handle.net/11449/248647 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadian Journal of Veterinary Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
97-104 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129192240349184 |