Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-5150-PVB-6259 http://hdl.handle.net/11449/200867 |
Resumo: | The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil. |
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Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitisBovine mastitisCattleMicrobiologyMilkMolecular detectionMollicutesMycoplasma bovisMycoplasmosisPCRThe genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Stiftung Professor Dr. Max CloëttaDepartamento de Higiene e Veterinária e Saúde Pública Universidade Estadual Paulista (Unesp), Rua Doutor Walter Maurício Correa s/n, Distrito de Rubião JúniorInstituto de Ciências Biomédicas Departamento de Microbiologia Universidade de São Paulo (USP), Av. Prof. Lineu Prestes 1374Veterinarian Qualy and Calf Consultoria Veterinária, Rua Vereador Alcides da Silva Reis 41Departamento de Higiene e Veterinária e Saúde Pública Universidade Estadual Paulista (Unesp), Rua Doutor Walter Maurício Correa s/n, Distrito de Rubião JúniorUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Qualy and Calf Consultoria VeterináriaSalina, Anelise [UNESP]Timenetsky, JorgeBarbosa, Maysa S.Azevedo, Cristiane M.Langoni, Helio [UNESP]2020-12-12T02:18:10Z2020-12-12T02:18:10Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article82-87application/pdfhttp://dx.doi.org/10.1590/1678-5150-PVB-6259Pesquisa Veterinaria Brasileira, v. 40, n. 2, p. 82-87, 2020.1678-51500100-736Xhttp://hdl.handle.net/11449/20086710.1590/1678-5150-PVB-6259S0100-736X20200002000822-s2.0-85089111157S0100-736X2020000200082.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-12-28T06:15:27Zoai:repositorio.unesp.br:11449/200867Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-28T06:15:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
title |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
spellingShingle |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis Salina, Anelise [UNESP] Bovine mastitis Cattle Microbiology Milk Molecular detection Mollicutes Mycoplasma bovis Mycoplasmosis PCR |
title_short |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
title_full |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
title_fullStr |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
title_full_unstemmed |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
title_sort |
Microbiological and molecular detection of Mycoplasma bovis in milk samples from bovine clinical mastitis |
author |
Salina, Anelise [UNESP] |
author_facet |
Salina, Anelise [UNESP] Timenetsky, Jorge Barbosa, Maysa S. Azevedo, Cristiane M. Langoni, Helio [UNESP] |
author_role |
author |
author2 |
Timenetsky, Jorge Barbosa, Maysa S. Azevedo, Cristiane M. Langoni, Helio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Qualy and Calf Consultoria Veterinária |
dc.contributor.author.fl_str_mv |
Salina, Anelise [UNESP] Timenetsky, Jorge Barbosa, Maysa S. Azevedo, Cristiane M. Langoni, Helio [UNESP] |
dc.subject.por.fl_str_mv |
Bovine mastitis Cattle Microbiology Milk Molecular detection Mollicutes Mycoplasma bovis Mycoplasmosis PCR |
topic |
Bovine mastitis Cattle Microbiology Milk Molecular detection Mollicutes Mycoplasma bovis Mycoplasmosis PCR |
description |
The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated from milk samples of bovine clinical mastitis, and to compare the isolation rates in two culture media: Hayflick and SP4. An initial screening was performed in order to detect the presence of the class Mollicutes in 1166 milk samples from clinical mastitis by the conventional Polymerase Chain Reaction (PCR) technique. According to the 1166 milk samples evaluated, 8.6% (100/1166) were positive to class Mollicutes. Regarding molecular analyses, 1.1% (13/1166) of conventional PCR for positive M. bovis was obtained and 0.9% (11/1166) in real-time PCR. The results of the microbiological culture of the 100 samples previously screened demonstrated that 6% (6/100) of colony growth have been developed when using the Hayflick medium, and 11% (11/100) when using the SP4 medium (including the positive on Hayflick medium). Concerning the 11 isolates obtained in the microbiological culture, conventional PCR confirmed M. bovis in nine of them, and two cultures were negative. In the phylogenetic analysis of the isolates, all of them were grouped in M. bovis and M. agalactiae clusters. The results confirmed the importance of the presence of M. bovis in the etiology of bovine clinical mastitis and reinforced the need for further studies to elucidate other Mycoplasma species that may be involved in bovine clinical mastitis in Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:18:10Z 2020-12-12T02:18:10Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-5150-PVB-6259 Pesquisa Veterinaria Brasileira, v. 40, n. 2, p. 82-87, 2020. 1678-5150 0100-736X http://hdl.handle.net/11449/200867 10.1590/1678-5150-PVB-6259 S0100-736X2020000200082 2-s2.0-85089111157 S0100-736X2020000200082.pdf |
url |
http://dx.doi.org/10.1590/1678-5150-PVB-6259 http://hdl.handle.net/11449/200867 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 40, n. 2, p. 82-87, 2020. 1678-5150 0100-736X 10.1590/1678-5150-PVB-6259 S0100-736X2020000200082 2-s2.0-85089111157 S0100-736X2020000200082.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
82-87 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790129727733760 |