Microbiomes associated with bovine periodontitis and oral health
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetmic.2018.03.016 http://hdl.handle.net/11449/179684 |
Resumo: | Periodontitis is an infectious polymicrobial, immuno-inflammatory disease of multifactorial aetiology that has an impact on the health, production and welfare of ruminants. The objective of the present study was to determine the microbial profiles present in the gingival sulcus of cattle considered periodontally healthy and in the periodontal pocket of animals with periodontitis lesions using high-throughput bacterial 16S rRNA gene sequencing. Subgingival biofilm samples were collected from 40 cattle with periodontitis and 38 periodontally healthy animals. In total, 1923 OTUs were identified and classified into 395 genera or higher taxa. Microbial profiles in health differed significantly from periodontitis in their composition (p < 0.0001, F = 5.30; PERMANOVA) but no statistically significant differences were observed in the diversity of healthy and periodontitis microbiomes. The most prevalent taxa in health were Pseudomonas, Burkholderia and Actinobacteria, whereas in disease these were Prevotella, Fusobacterium and Porphyromonas. The most discriminative taxa in health were Gastranaerophilales, Planifilum and Burkholderia, and in disease these were Elusimicrobia, Synergistes and Propionivibrio. In conclusion, statistically significant difference exists between the microbiome in bovine oral health and periodontitis, with populations showing 72.6% dissimilarity. The diversity of the bacteria found in health and periodontitis were similar and bacteria recognised as periodontal pathogens showed increased abundance in disease. In this context, the main components of bacterial homeostasis in the biofilm of healthy sites and of dysbiosis in periodontal lesions provide unprecedented indicators for the evolution of knowledge about bovine periodontitis. |
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Microbiomes associated with bovine periodontitis and oral healthBacteriaCattleHigh-throughput sequencingMicrobiomePeriodontal diseasePeriodontitis is an infectious polymicrobial, immuno-inflammatory disease of multifactorial aetiology that has an impact on the health, production and welfare of ruminants. The objective of the present study was to determine the microbial profiles present in the gingival sulcus of cattle considered periodontally healthy and in the periodontal pocket of animals with periodontitis lesions using high-throughput bacterial 16S rRNA gene sequencing. Subgingival biofilm samples were collected from 40 cattle with periodontitis and 38 periodontally healthy animals. In total, 1923 OTUs were identified and classified into 395 genera or higher taxa. Microbial profiles in health differed significantly from periodontitis in their composition (p < 0.0001, F = 5.30; PERMANOVA) but no statistically significant differences were observed in the diversity of healthy and periodontitis microbiomes. The most prevalent taxa in health were Pseudomonas, Burkholderia and Actinobacteria, whereas in disease these were Prevotella, Fusobacterium and Porphyromonas. The most discriminative taxa in health were Gastranaerophilales, Planifilum and Burkholderia, and in disease these were Elusimicrobia, Synergistes and Propionivibrio. In conclusion, statistically significant difference exists between the microbiome in bovine oral health and periodontitis, with populations showing 72.6% dissimilarity. The diversity of the bacteria found in health and periodontitis were similar and bacteria recognised as periodontal pathogens showed increased abundance in disease. In this context, the main components of bacterial homeostasis in the biofilm of healthy sites and of dysbiosis in periodontal lesions provide unprecedented indicators for the evolution of knowledge about bovine periodontitis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Veterinary Medicine, Araçatuba CampusDental School University of GlasgowSchool of Veterinary Medicine University of GlasgowDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and VU University AmsterdamSão Paulo State University (Unesp) School of Veterinary Medicine, Araçatuba CampusUniversidade Estadual Paulista (Unesp)University of GlasgowUniversity of Amsterdam and VU University AmsterdamBorsanelli, Ana C. [UNESP]Lappin, David F.Viora, LorenzoBennett, DavidDutra, Iveraldo S. [UNESP]Brandt, Bernd W.Riggio, Marcello P.2018-12-11T17:36:21Z2018-12-11T17:36:21Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-6application/pdfhttp://dx.doi.org/10.1016/j.vetmic.2018.03.016Veterinary Microbiology, v. 218, p. 1-6.1873-25420378-1135http://hdl.handle.net/11449/17968410.1016/j.vetmic.2018.03.0162-s2.0-850441668552-s2.0-85044166855.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiology1,175info:eu-repo/semantics/openAccess2024-09-04T19:15:39Zoai:repositorio.unesp.br:11449/179684Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microbiomes associated with bovine periodontitis and oral health |
title |
Microbiomes associated with bovine periodontitis and oral health |
spellingShingle |
Microbiomes associated with bovine periodontitis and oral health Borsanelli, Ana C. [UNESP] Bacteria Cattle High-throughput sequencing Microbiome Periodontal disease |
title_short |
Microbiomes associated with bovine periodontitis and oral health |
title_full |
Microbiomes associated with bovine periodontitis and oral health |
title_fullStr |
Microbiomes associated with bovine periodontitis and oral health |
title_full_unstemmed |
Microbiomes associated with bovine periodontitis and oral health |
title_sort |
Microbiomes associated with bovine periodontitis and oral health |
author |
Borsanelli, Ana C. [UNESP] |
author_facet |
Borsanelli, Ana C. [UNESP] Lappin, David F. Viora, Lorenzo Bennett, David Dutra, Iveraldo S. [UNESP] Brandt, Bernd W. Riggio, Marcello P. |
author_role |
author |
author2 |
Lappin, David F. Viora, Lorenzo Bennett, David Dutra, Iveraldo S. [UNESP] Brandt, Bernd W. Riggio, Marcello P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Glasgow University of Amsterdam and VU University Amsterdam |
dc.contributor.author.fl_str_mv |
Borsanelli, Ana C. [UNESP] Lappin, David F. Viora, Lorenzo Bennett, David Dutra, Iveraldo S. [UNESP] Brandt, Bernd W. Riggio, Marcello P. |
dc.subject.por.fl_str_mv |
Bacteria Cattle High-throughput sequencing Microbiome Periodontal disease |
topic |
Bacteria Cattle High-throughput sequencing Microbiome Periodontal disease |
description |
Periodontitis is an infectious polymicrobial, immuno-inflammatory disease of multifactorial aetiology that has an impact on the health, production and welfare of ruminants. The objective of the present study was to determine the microbial profiles present in the gingival sulcus of cattle considered periodontally healthy and in the periodontal pocket of animals with periodontitis lesions using high-throughput bacterial 16S rRNA gene sequencing. Subgingival biofilm samples were collected from 40 cattle with periodontitis and 38 periodontally healthy animals. In total, 1923 OTUs were identified and classified into 395 genera or higher taxa. Microbial profiles in health differed significantly from periodontitis in their composition (p < 0.0001, F = 5.30; PERMANOVA) but no statistically significant differences were observed in the diversity of healthy and periodontitis microbiomes. The most prevalent taxa in health were Pseudomonas, Burkholderia and Actinobacteria, whereas in disease these were Prevotella, Fusobacterium and Porphyromonas. The most discriminative taxa in health were Gastranaerophilales, Planifilum and Burkholderia, and in disease these were Elusimicrobia, Synergistes and Propionivibrio. In conclusion, statistically significant difference exists between the microbiome in bovine oral health and periodontitis, with populations showing 72.6% dissimilarity. The diversity of the bacteria found in health and periodontitis were similar and bacteria recognised as periodontal pathogens showed increased abundance in disease. In this context, the main components of bacterial homeostasis in the biofilm of healthy sites and of dysbiosis in periodontal lesions provide unprecedented indicators for the evolution of knowledge about bovine periodontitis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:36:21Z 2018-12-11T17:36:21Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetmic.2018.03.016 Veterinary Microbiology, v. 218, p. 1-6. 1873-2542 0378-1135 http://hdl.handle.net/11449/179684 10.1016/j.vetmic.2018.03.016 2-s2.0-85044166855 2-s2.0-85044166855.pdf |
url |
http://dx.doi.org/10.1016/j.vetmic.2018.03.016 http://hdl.handle.net/11449/179684 |
identifier_str_mv |
Veterinary Microbiology, v. 218, p. 1-6. 1873-2542 0378-1135 10.1016/j.vetmic.2018.03.016 2-s2.0-85044166855 2-s2.0-85044166855.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Microbiology 1,175 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-6 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021397037056000 |