A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P139-145 http://hdl.handle.net/11449/232977 |
Resumo: | A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer´s law is obeyed in a concentration ranges 1.03 x 10-5 to 3.61 x 10–4 mol L-1 and 1.75 x 10-5 to 3.48 x 10–4 mol L-1 for the tetracycline and doxycycline, respectively. The quantification limits were 5.63 x 10-6 mol L-1 and 7.12 x 10-7 mol L-1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level. |
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A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-TChloramine-TDoxycyclineSpectrophotometricTetracyclineA simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer´s law is obeyed in a concentration ranges 1.03 x 10-5 to 3.61 x 10–4 mol L-1 and 1.75 x 10-5 to 3.48 x 10–4 mol L-1 for the tetracycline and doxycycline, respectively. The quantification limits were 5.63 x 10-6 mol L-1 and 7.12 x 10-7 mol L-1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.São Paulo State University - UNESP Institute of Chemistry of Araraquara, P.O. Box 355São Paulo State University - UNESP Institute of Chemistry of Araraquara, P.O. Box 355Universidade Estadual Paulista (UNESP)Rufino, José L. [UNESP]Fernandes, Flávio C.B. [UNESP]Ruy, Mayara S. [UNESP]Pezza, Helena R. [UNESP]Pezza, Leonardo [UNESP]2022-04-30T22:56:31Z2022-04-30T22:56:31Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article139-146http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P139-145Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010.1678-46180100-4670http://hdl.handle.net/11449/23297710.26850/1678-4618EQJ.V35.4.2010.P139-1452-s2.0-85082366564Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcletica Quimicainfo:eu-repo/semantics/openAccess2022-04-30T22:56:31Zoai:repositorio.unesp.br:11449/232977Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-30T22:56:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
title |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
spellingShingle |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T Rufino, José L. [UNESP] Chloramine-T Doxycycline Spectrophotometric Tetracycline |
title_short |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
title_full |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
title_fullStr |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
title_full_unstemmed |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
title_sort |
A simple spectrophotometric method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T |
author |
Rufino, José L. [UNESP] |
author_facet |
Rufino, José L. [UNESP] Fernandes, Flávio C.B. [UNESP] Ruy, Mayara S. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
author_role |
author |
author2 |
Fernandes, Flávio C.B. [UNESP] Ruy, Mayara S. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Rufino, José L. [UNESP] Fernandes, Flávio C.B. [UNESP] Ruy, Mayara S. [UNESP] Pezza, Helena R. [UNESP] Pezza, Leonardo [UNESP] |
dc.subject.por.fl_str_mv |
Chloramine-T Doxycycline Spectrophotometric Tetracycline |
topic |
Chloramine-T Doxycycline Spectrophotometric Tetracycline |
description |
A simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the ë = 535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer´s law is obeyed in a concentration ranges 1.03 x 10-5 to 3.61 x 10–4 mol L-1 and 1.75 x 10-5 to 3.48 x 10–4 mol L-1 for the tetracycline and doxycycline, respectively. The quantification limits were 5.63 x 10-6 mol L-1 and 7.12 x 10-7 mol L-1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2022-04-30T22:56:31Z 2022-04-30T22:56:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P139-145 Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010. 1678-4618 0100-4670 http://hdl.handle.net/11449/232977 10.26850/1678-4618EQJ.V35.4.2010.P139-145 2-s2.0-85082366564 |
url |
http://dx.doi.org/10.26850/1678-4618EQJ.V35.4.2010.P139-145 http://hdl.handle.net/11449/232977 |
identifier_str_mv |
Ecletica Quimica, v. 35, n. 4, p. 139-146, 2010. 1678-4618 0100-4670 10.26850/1678-4618EQJ.V35.4.2010.P139-145 2-s2.0-85082366564 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecletica Quimica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
139-146 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790423406608384 |