Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations

Detalhes bibliográficos
Autor(a) principal: Raza,Asad
Data de Publicação: 2009
Outros Autores: Ijaz,Abdul Subhan, Ahmad,Shabbir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000500018
Resumo: A simple, fast and sensitive spectrophotometric method for the determination of cefaclor in pharmaceutical raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn's purple) that resulted from the reaction was stabilized and measured at 560 nm. Beer's law is obeyed in the concentration range of 4-80 µg mL-1 with molar absorptivity of 1.42 × 10(5) L mole-1 cm-1. All variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.
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spelling Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulationsspectrophotometricCefaclorpharmaceutical formulationsA simple, fast and sensitive spectrophotometric method for the determination of cefaclor in pharmaceutical raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn's purple) that resulted from the reaction was stabilized and measured at 560 nm. Beer's law is obeyed in the concentration range of 4-80 µg mL-1 with molar absorptivity of 1.42 × 10(5) L mole-1 cm-1. All variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000500018Química Nova v.32 n.5 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000500018info:eu-repo/semantics/openAccessRaza,AsadIjaz,Abdul SubhanAhmad,Shabbireng2009-08-04T00:00:00Zoai:scielo:S0100-40422009000500018Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-08-04T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
title Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
spellingShingle Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
Raza,Asad
spectrophotometric
Cefaclor
pharmaceutical formulations
title_short Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
title_full Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
title_fullStr Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
title_full_unstemmed Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
title_sort Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations
author Raza,Asad
author_facet Raza,Asad
Ijaz,Abdul Subhan
Ahmad,Shabbir
author_role author
author2 Ijaz,Abdul Subhan
Ahmad,Shabbir
author2_role author
author
dc.contributor.author.fl_str_mv Raza,Asad
Ijaz,Abdul Subhan
Ahmad,Shabbir
dc.subject.por.fl_str_mv spectrophotometric
Cefaclor
pharmaceutical formulations
topic spectrophotometric
Cefaclor
pharmaceutical formulations
description A simple, fast and sensitive spectrophotometric method for the determination of cefaclor in pharmaceutical raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn's purple) that resulted from the reaction was stabilized and measured at 560 nm. Beer's law is obeyed in the concentration range of 4-80 µg mL-1 with molar absorptivity of 1.42 × 10(5) L mole-1 cm-1. All variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000500018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000500018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422009000500018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.32 n.5 2009
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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