Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs

Detalhes bibliográficos
Autor(a) principal: Paula, Luiza F. A. de
Data de Publicação: 2017
Outros Autores: Leal, Barbara S. S. [UNESP], Rexroth, Juliane, Porembski, Stefan, Palma-Silva, Clarisse [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s40415-017-0396-x
http://hdl.handle.net/11449/163561
Resumo: Inselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists.
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spelling Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergsConservation geneticsCross-amplificationMonocotsPopulation geneticsInselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists.DAAD (Deutscher Akademischer Austauschdienst)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Rostock, Inst Biowissensch Allgemeine & Spezielle Bot, Wismarsche Str 44-45, D-18051 Rostock, GermanyUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilDAAD (Deutscher Akademischer Austauschdienst): 290155/2014-2Soc Botanica Sao PauloUniv RostockUniversidade Estadual Paulista (Unesp)Paula, Luiza F. A. deLeal, Barbara S. S. [UNESP]Rexroth, JulianePorembski, StefanPalma-Silva, Clarisse [UNESP]2018-11-26T17:42:32Z2018-11-26T17:42:32Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1071-1075application/pdfhttp://dx.doi.org/10.1007/s40415-017-0396-xBrazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017.1806-9959http://hdl.handle.net/11449/16356110.1007/s40415-017-0396-xWOS:000417093400025WOS000417093400025.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Botanyinfo:eu-repo/semantics/openAccess2024-01-21T06:22:16Zoai:repositorio.unesp.br:11449/163561Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:12.802737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
title Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
spellingShingle Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
Paula, Luiza F. A. de
Conservation genetics
Cross-amplification
Monocots
Population genetics
title_short Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
title_full Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
title_fullStr Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
title_full_unstemmed Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
title_sort Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
author Paula, Luiza F. A. de
author_facet Paula, Luiza F. A. de
Leal, Barbara S. S. [UNESP]
Rexroth, Juliane
Porembski, Stefan
Palma-Silva, Clarisse [UNESP]
author_role author
author2 Leal, Barbara S. S. [UNESP]
Rexroth, Juliane
Porembski, Stefan
Palma-Silva, Clarisse [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Univ Rostock
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Paula, Luiza F. A. de
Leal, Barbara S. S. [UNESP]
Rexroth, Juliane
Porembski, Stefan
Palma-Silva, Clarisse [UNESP]
dc.subject.por.fl_str_mv Conservation genetics
Cross-amplification
Monocots
Population genetics
topic Conservation genetics
Cross-amplification
Monocots
Population genetics
description Inselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
2018-11-26T17:42:32Z
2018-11-26T17:42:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s40415-017-0396-x
Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017.
1806-9959
http://hdl.handle.net/11449/163561
10.1007/s40415-017-0396-x
WOS:000417093400025
WOS000417093400025.pdf
url http://dx.doi.org/10.1007/s40415-017-0396-x
http://hdl.handle.net/11449/163561
identifier_str_mv Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017.
1806-9959
10.1007/s40415-017-0396-x
WOS:000417093400025
WOS000417093400025.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Botany
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1071-1075
application/pdf
dc.publisher.none.fl_str_mv Soc Botanica Sao Paulo
publisher.none.fl_str_mv Soc Botanica Sao Paulo
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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