Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s40415-017-0396-x http://hdl.handle.net/11449/163561 |
Resumo: | Inselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists. |
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Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergsConservation geneticsCross-amplificationMonocotsPopulation geneticsInselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists.DAAD (Deutscher Akademischer Austauschdienst)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Rostock, Inst Biowissensch Allgemeine & Spezielle Bot, Wismarsche Str 44-45, D-18051 Rostock, GermanyUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilDAAD (Deutscher Akademischer Austauschdienst): 290155/2014-2Soc Botanica Sao PauloUniv RostockUniversidade Estadual Paulista (Unesp)Paula, Luiza F. A. deLeal, Barbara S. S. [UNESP]Rexroth, JulianePorembski, StefanPalma-Silva, Clarisse [UNESP]2018-11-26T17:42:32Z2018-11-26T17:42:32Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1071-1075application/pdfhttp://dx.doi.org/10.1007/s40415-017-0396-xBrazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017.1806-9959http://hdl.handle.net/11449/16356110.1007/s40415-017-0396-xWOS:000417093400025WOS000417093400025.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Botanyinfo:eu-repo/semantics/openAccess2024-01-21T06:22:16Zoai:repositorio.unesp.br:11449/163561Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:12.802737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
title |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
spellingShingle |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs Paula, Luiza F. A. de Conservation genetics Cross-amplification Monocots Population genetics |
title_short |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
title_full |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
title_fullStr |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
title_full_unstemmed |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
title_sort |
Transferability of microsatellite loci to Vellozia plicata (Velloziaceae), a widespread species on Brazilian inselbergs |
author |
Paula, Luiza F. A. de |
author_facet |
Paula, Luiza F. A. de Leal, Barbara S. S. [UNESP] Rexroth, Juliane Porembski, Stefan Palma-Silva, Clarisse [UNESP] |
author_role |
author |
author2 |
Leal, Barbara S. S. [UNESP] Rexroth, Juliane Porembski, Stefan Palma-Silva, Clarisse [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Rostock Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Paula, Luiza F. A. de Leal, Barbara S. S. [UNESP] Rexroth, Juliane Porembski, Stefan Palma-Silva, Clarisse [UNESP] |
dc.subject.por.fl_str_mv |
Conservation genetics Cross-amplification Monocots Population genetics |
topic |
Conservation genetics Cross-amplification Monocots Population genetics |
description |
Inselbergs are isolated, mainly granitic and gneissic rock outcrops, forming old landscape elements on crystalline continental shields on all continents. Many Velloziaceae which occur specifically as mat-formers on these rock outcrops are well adapted to harsh environmental conditions and do not occur in the surrounding matrix. Vellozia plicata Mart. is a widespread desiccationtolerant mat-forming species on inselbergs in southeastern Brazil, with naturally fragmented populations. Here, we tested the transferability of 53 microsatellite loci previously developed for two Vellozia species (V. gigantea N. L. Menezes & Mello-Silva and V. squamata Pohl) in two populations of V. plicata. We succeeded in the amplification of 11 loci, and six of them were polymorphic. The number of alleles per locus ranged from 2 to 17, the expected and observed heterozygosities ranged from 0.000 to 0.875 and from 0.000 to 0.895, respectively. Our results show that cross-amplification works within the genus Vellozia, but at a lower level when compared to other monocot families on inselbergs, such as Bromeliaceae and Orchidaceae. This work might help in further studies on population genetics of V. plicata, in order to guide conservation actions in the future and also to promote further investigations about speciation processes of inselberg specialists. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-11-26T17:42:32Z 2018-11-26T17:42:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s40415-017-0396-x Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017. 1806-9959 http://hdl.handle.net/11449/163561 10.1007/s40415-017-0396-x WOS:000417093400025 WOS000417093400025.pdf |
url |
http://dx.doi.org/10.1007/s40415-017-0396-x http://hdl.handle.net/11449/163561 |
identifier_str_mv |
Brazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 40, n. 4, p. 1071-1075, 2017. 1806-9959 10.1007/s40415-017-0396-x WOS:000417093400025 WOS000417093400025.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Botany |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1071-1075 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Botanica Sao Paulo |
publisher.none.fl_str_mv |
Soc Botanica Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129532829368320 |