Breast cancer detection using charge sensors coupled to DNA monolayer

Detalhes bibliográficos
Autor(a) principal: Batistuti, Marina R.
Data de Publicação: 2015
Outros Autores: Mulato, Marcelo, Bueno, Paulo R. [UNESP]
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1557/opl.2015.671
http://hdl.handle.net/11449/234435
Resumo: We report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (R) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCb influence on s,DNA immobilization solution.
id UNSP_6605f593cd23bc8191be941dacc9651b
oai_identifier_str oai:repositorio.unesp.br:11449/234435
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Breast cancer detection using charge sensors coupled to DNA monolayerWe report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (R) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCb influence on s,DNA immobilization solution.Departamento de Fisica Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto Universidade de Sao PauloInstituto de Quimica Universidade Estadual Paulista, CP 355Instituto de Quimica Universidade Estadual Paulista, CP 355Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Batistuti, Marina R.Mulato, MarceloBueno, Paulo R. [UNESP]2022-05-02T17:01:39Z2022-05-02T17:01:39Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject19-26http://dx.doi.org/10.1557/opl.2015.671Materials Research Society Symposium Proceedings, v. 1793, p. 19-26.0272-9172http://hdl.handle.net/11449/23443510.1557/opl.2015.6712-s2.0-84985961525Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Research Society Symposium Proceedingsinfo:eu-repo/semantics/openAccess2022-05-02T17:01:39Zoai:repositorio.unesp.br:11449/234435Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:36:01.719826Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Breast cancer detection using charge sensors coupled to DNA monolayer
title Breast cancer detection using charge sensors coupled to DNA monolayer
spellingShingle Breast cancer detection using charge sensors coupled to DNA monolayer
Batistuti, Marina R.
title_short Breast cancer detection using charge sensors coupled to DNA monolayer
title_full Breast cancer detection using charge sensors coupled to DNA monolayer
title_fullStr Breast cancer detection using charge sensors coupled to DNA monolayer
title_full_unstemmed Breast cancer detection using charge sensors coupled to DNA monolayer
title_sort Breast cancer detection using charge sensors coupled to DNA monolayer
author Batistuti, Marina R.
author_facet Batistuti, Marina R.
Mulato, Marcelo
Bueno, Paulo R. [UNESP]
author_role author
author2 Mulato, Marcelo
Bueno, Paulo R. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Batistuti, Marina R.
Mulato, Marcelo
Bueno, Paulo R. [UNESP]
description We report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (R) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCb influence on s,DNA immobilization solution.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2022-05-02T17:01:39Z
2022-05-02T17:01:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1557/opl.2015.671
Materials Research Society Symposium Proceedings, v. 1793, p. 19-26.
0272-9172
http://hdl.handle.net/11449/234435
10.1557/opl.2015.671
2-s2.0-84985961525
url http://dx.doi.org/10.1557/opl.2015.671
http://hdl.handle.net/11449/234435
identifier_str_mv Materials Research Society Symposium Proceedings, v. 1793, p. 19-26.
0272-9172
10.1557/opl.2015.671
2-s2.0-84985961525
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Research Society Symposium Proceedings
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19-26
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128834000650240

Registros relacionados