Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1

• Autor(a) principal: Tamayo, Abel I. Balbin
• Data de Publicação: 2020
• Outros Autores: Rizo, Laura S. López, de Armas, Madelin Blanco, Ferreira, Antonio Aparecido Pupim [UNESP], Manzani, Danilo, Yamanaka, Hideko [UNESP], Guas, Ana Margarita Esteva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.microc.2019.104462; http://hdl.handle.net/11449/201371
• topic: DNA biosensors; Genosensor; HIV-1; Hybridization; Impedimetric; Proviral DNA

This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.