A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2147/IJN.S82385 http://hdl.handle.net/11449/131389 |
Resumo: | Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis. |
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A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasisCandida albicansLiquid crystalline systemsMucoadhesive polymersNanostructured drug delivery systemsVaginal administrationWomen often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis.School of Pharmaceutical Sciences, UNESP - Sao Paulo State University, Campus Araraquara, Department of Drugs and Medicines, Araraquara, Sao Paulo, Brazil.School of Pharmaceutical Sciences, UNESP - Sao Paulo State University, Campus Araraquara, Department of Drugs and Medicines, Araraquara, Sao Paulo, Brazil.International Journal Of NanomedicineUniversidade Estadual Paulista (Unesp)Salmazi, Rafael [UNESP]Calixto, Giovana [UNESP]Bernegossi, Jéssica [UNESP]Ramos, Matheus Aparecido dos Santos [UNESP]Bauab, Taís Maria [UNESP]Chorilli, Marlus [UNESP]2015-12-07T15:34:41Z2015-12-07T15:34:41Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4815-4824application/pdfhttp://dx.doi.org/10.2147/IJN.S82385International Journal Of Nanomedicine, v. 10, p. 4815-4824, 2015.1178-2013http://hdl.handle.net/11449/13138910.2147/IJN.S82385PMC4525803.pdf4910754838277580142712599671628226257519PMC4525803PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Nanomedicine4.3701,225info:eu-repo/semantics/openAccess2024-06-24T13:44:55Zoai:repositorio.unesp.br:11449/131389Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:09:17.321913Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
title |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
spellingShingle |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis Salmazi, Rafael [UNESP] Candida albicans Liquid crystalline systems Mucoadhesive polymers Nanostructured drug delivery systems Vaginal administration |
title_short |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
title_full |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
title_fullStr |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
title_full_unstemmed |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
title_sort |
A curcumin-loaded liquid crystal precursor mucoadhesive system for the treatment of vaginal candidiasis |
author |
Salmazi, Rafael [UNESP] |
author_facet |
Salmazi, Rafael [UNESP] Calixto, Giovana [UNESP] Bernegossi, Jéssica [UNESP] Ramos, Matheus Aparecido dos Santos [UNESP] Bauab, Taís Maria [UNESP] Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Calixto, Giovana [UNESP] Bernegossi, Jéssica [UNESP] Ramos, Matheus Aparecido dos Santos [UNESP] Bauab, Taís Maria [UNESP] Chorilli, Marlus [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Salmazi, Rafael [UNESP] Calixto, Giovana [UNESP] Bernegossi, Jéssica [UNESP] Ramos, Matheus Aparecido dos Santos [UNESP] Bauab, Taís Maria [UNESP] Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans Liquid crystalline systems Mucoadhesive polymers Nanostructured drug delivery systems Vaginal administration |
topic |
Candida albicans Liquid crystalline systems Mucoadhesive polymers Nanostructured drug delivery systems Vaginal administration |
description |
Women often develop vaginal infections that are caused primarily by organisms of the genus Candida. The current treatments of vaginal candidiasis usually involve azole-based antifungals, though fungal resistance to these compounds has become prevalent. Therefore, much attention has been given to molecules with antifungal properties from natural sources, such as curcumin (CUR). However, CUR has poor solubility in aqueous solvents and poor oral bioavailability. This study attempted to overcome this problem by developing, characterizing, and evaluating the in vitro antifungal action of a CUR-loaded liquid crystal precursor mucoadhesive system (LCPM) for vaginal administration. A low-viscosity LCPM (F) consisting of 40% wt/wt polyoxpropylene-(5)-polyoxyethylene-(20)-cetyl alcohol, 50% wt/wt oleic acid, and 10% wt/wt chitosan dispersion at 0.5% with the addition of 16% poloxamer 407 was developed to take advantage of the lyotropic phase behavior of this formulation. Notably, F could transform into liquid crystal systems when diluted with artificial vaginal mucus at ratios of 1:3 and 1:1 (wt/wt), resulting in the formation of F30 and F100, respectively. Polarized light microscopy and rheological studies revealed that F behaved like an isotropic formulation, whereas F30 and F100 behaved like an anisotropic liquid crystalline system (LCS). Moreover, F30 and F100 presented higher mucoadhesion to porcine vaginal mucosa than F. The analysis of the in vitro activity against Candida albicans revealed that CUR-loaded F was more potent against standard and clinical strains compared with a CUR solution. Therefore, the vaginal administration of CUR-loaded LCPMs represents a promising platform for the treatment of vaginal candidiasis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:34:41Z 2015-12-07T15:34:41Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2147/IJN.S82385 International Journal Of Nanomedicine, v. 10, p. 4815-4824, 2015. 1178-2013 http://hdl.handle.net/11449/131389 10.2147/IJN.S82385 PMC4525803.pdf 4910754838277580 1427125996716282 26257519 PMC4525803 |
url |
http://dx.doi.org/10.2147/IJN.S82385 http://hdl.handle.net/11449/131389 |
identifier_str_mv |
International Journal Of Nanomedicine, v. 10, p. 4815-4824, 2015. 1178-2013 10.2147/IJN.S82385 PMC4525803.pdf 4910754838277580 1427125996716282 26257519 PMC4525803 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Nanomedicine 4.370 1,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4815-4824 application/pdf |
dc.publisher.none.fl_str_mv |
International Journal Of Nanomedicine |
publisher.none.fl_str_mv |
International Journal Of Nanomedicine |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128323143860224 |