Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.resp.2016.08.004 http://hdl.handle.net/11449/162101 |
Resumo: | The locus coeruleus (LC) is a pontine noradrenergic nucleus that acts as a central chemoreceptor to CO2/pH and has been implicated in the cognitive aspects of stress responses. This participation is in part mediated by the action of corticotropin-releasing factor (CRF), which when released in these situations increases the firing frequency of LC noradrenergic neurons. Nevertheless, the role of CRF1 receptors in the LC in breathing and temperature control is unknown. Therefore, we tested the involvement of CRF1 receptors located in the LC in room air ventilation and the ventilatory response induced by hypercapnia (7% CO2) in rats. To this end, we injected CRF-R1-selective antagonists (antalarmin-1.2 and 2.4 mmol/0.1 mu L, or CP-376395-5 nmol/0.1 mu L) into the LC of male Wistar rats. Pulmonary ventilation (V-E) and body temperature (Tb, dataloggers) were measured in air, followed by 7% CO2 in unanesthetized rats. Antalarmin (higher dose) and CP-376395 in the LC caused an increase in V-E during normocapnia and hypercapnia, due to an increase in tidal volume. There were no differences in Tb between groups under normocapnia and hypercapnia. The results suggest that CRF acting on CRF1 receptors in the LC exerts a tonic inhibitory role in ventilation. (C) 2016 Elsevier B.V. All rights reserved. |
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Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in ratsLocus coeruleusCRF receptorsHypercapniaVentilationChemoreceptionThe locus coeruleus (LC) is a pontine noradrenergic nucleus that acts as a central chemoreceptor to CO2/pH and has been implicated in the cognitive aspects of stress responses. This participation is in part mediated by the action of corticotropin-releasing factor (CRF), which when released in these situations increases the firing frequency of LC noradrenergic neurons. Nevertheless, the role of CRF1 receptors in the LC in breathing and temperature control is unknown. Therefore, we tested the involvement of CRF1 receptors located in the LC in room air ventilation and the ventilatory response induced by hypercapnia (7% CO2) in rats. To this end, we injected CRF-R1-selective antagonists (antalarmin-1.2 and 2.4 mmol/0.1 mu L, or CP-376395-5 nmol/0.1 mu L) into the LC of male Wistar rats. Pulmonary ventilation (V-E) and body temperature (Tb, dataloggers) were measured in air, followed by 7% CO2 in unanesthetized rats. Antalarmin (higher dose) and CP-376395 in the LC caused an increase in V-E during normocapnia and hypercapnia, due to an increase in tidal volume. There were no differences in Tb between groups under normocapnia and hypercapnia. The results suggest that CRF acting on CRF1 receptors in the LC exerts a tonic inhibitory role in ventilation. (C) 2016 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP, Fac Ciencias Agr & Vet, Dept Morfol & Fisiol Anim, Rod Prof Paulo Donato Castellane S-N, BR-14870000 Jaboticabal, SP, BrazilNatl Inst Sci & Technol Comparat Physiol, Sao Paulo, BrazilUNESP, Fac Ciencias Agr & Vet, Dept Morfol & Fisiol Anim, Rod Prof Paulo Donato Castellane S-N, BR-14870000 Jaboticabal, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Natl Inst Sci & Technol Comparat PhysiolIncheglu, Jolene M. [UNESP]Bicego, Kenia Cardoso [UNESP]Gargaglioni, Luciane H. [UNESP]2018-11-26T17:10:21Z2018-11-26T17:10:21Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article73-80application/pdfhttp://dx.doi.org/10.1016/j.resp.2016.08.004Respiratory Physiology & Neurobiology. Amsterdam: Elsevier Science Bv, v. 233, p. 73-80, 2016.1569-9048http://hdl.handle.net/11449/16210110.1016/j.resp.2016.08.004WOS:000386410600011WOS000386410600011.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRespiratory Physiology & Neurobiology0,682info:eu-repo/semantics/openAccess2024-06-06T18:42:13Zoai:repositorio.unesp.br:11449/162101Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:01:03.675392Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
title |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
spellingShingle |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats Incheglu, Jolene M. [UNESP] Locus coeruleus CRF receptors Hypercapnia Ventilation Chemoreception |
title_short |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
title_full |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
title_fullStr |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
title_full_unstemmed |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
title_sort |
Corticotropin-releasing factor in the locus coeruleus as a modulator of ventilation in rats |
author |
Incheglu, Jolene M. [UNESP] |
author_facet |
Incheglu, Jolene M. [UNESP] Bicego, Kenia Cardoso [UNESP] Gargaglioni, Luciane H. [UNESP] |
author_role |
author |
author2 |
Bicego, Kenia Cardoso [UNESP] Gargaglioni, Luciane H. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Natl Inst Sci & Technol Comparat Physiol |
dc.contributor.author.fl_str_mv |
Incheglu, Jolene M. [UNESP] Bicego, Kenia Cardoso [UNESP] Gargaglioni, Luciane H. [UNESP] |
dc.subject.por.fl_str_mv |
Locus coeruleus CRF receptors Hypercapnia Ventilation Chemoreception |
topic |
Locus coeruleus CRF receptors Hypercapnia Ventilation Chemoreception |
description |
The locus coeruleus (LC) is a pontine noradrenergic nucleus that acts as a central chemoreceptor to CO2/pH and has been implicated in the cognitive aspects of stress responses. This participation is in part mediated by the action of corticotropin-releasing factor (CRF), which when released in these situations increases the firing frequency of LC noradrenergic neurons. Nevertheless, the role of CRF1 receptors in the LC in breathing and temperature control is unknown. Therefore, we tested the involvement of CRF1 receptors located in the LC in room air ventilation and the ventilatory response induced by hypercapnia (7% CO2) in rats. To this end, we injected CRF-R1-selective antagonists (antalarmin-1.2 and 2.4 mmol/0.1 mu L, or CP-376395-5 nmol/0.1 mu L) into the LC of male Wistar rats. Pulmonary ventilation (V-E) and body temperature (Tb, dataloggers) were measured in air, followed by 7% CO2 in unanesthetized rats. Antalarmin (higher dose) and CP-376395 in the LC caused an increase in V-E during normocapnia and hypercapnia, due to an increase in tidal volume. There were no differences in Tb between groups under normocapnia and hypercapnia. The results suggest that CRF acting on CRF1 receptors in the LC exerts a tonic inhibitory role in ventilation. (C) 2016 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T17:10:21Z 2018-11-26T17:10:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.resp.2016.08.004 Respiratory Physiology & Neurobiology. Amsterdam: Elsevier Science Bv, v. 233, p. 73-80, 2016. 1569-9048 http://hdl.handle.net/11449/162101 10.1016/j.resp.2016.08.004 WOS:000386410600011 WOS000386410600011.pdf |
url |
http://dx.doi.org/10.1016/j.resp.2016.08.004 http://hdl.handle.net/11449/162101 |
identifier_str_mv |
Respiratory Physiology & Neurobiology. Amsterdam: Elsevier Science Bv, v. 233, p. 73-80, 2016. 1569-9048 10.1016/j.resp.2016.08.004 WOS:000386410600011 WOS000386410600011.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Respiratory Physiology & Neurobiology 0,682 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
73-80 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129274467581952 |