Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.karger.com/Article/FullText/369683 http://hdl.handle.net/11449/128297 |
Resumo: | Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity. |
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Repositório Institucional da UNESP |
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Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure ratsHeart failureSkeletal muscleNADPH oxidaseMyocardial infarctionN-acetylcysteineReactive oxygen speciesBackground: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade de São Paulo, Instituto do Coração do Hospital das Clínicas, Faculdade de MedicinaUniversidade Estadual Paulista, Departamento de Clínica Médica, Faculdade de Medicina de BotucatuFAPESP: 2010/50461-6FAPESP: 2007/59500-1CNPq: 306857/2012-0CNPq: 306.845/2012-1KargerUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual do Norte do Paraná (UENP)Universidade de São Paulo (USP)Martinez, Paula Felippe [UNESP]Bonomo, Camila [UNESP]Guizoni, Daniele Mendes [UNESP]Oliveira Junior, Silvio Assis [UNESP]Damatto, Ricardo Luiz [UNESP]Cezar, Marcelo Diarcadia Mariano [UNESP]Lima, Aline Regina Ruiz [UNESP]Pagan, Luana Urbano [UNESP]Seiva, Fabio RodriguesFernandes, Denise CastroLaurindo, Francisco Rafael MartinsNovelli, Ethel Lourenzi Barbosa [UNESP]Matsubara, Luiz Shiguero [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Okoshi, Katashi [UNESP]Okoshi, Marina Politi [UNESP]2015-10-21T13:08:47Z2015-10-21T13:08:47Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-159application/pdfhttp://www.karger.com/Article/FullText/369683Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015.1015-8987http://hdl.handle.net/11449/12829710.1159/000369683WOS:000348048000014WOS000348048000014.pdf6309835137998766159097157630942044631386719984325016839015394547Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology And Biochemistry5.5001,561info:eu-repo/semantics/openAccess2024-08-14T17:23:43Zoai:repositorio.unesp.br:11449/128297Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
title |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
spellingShingle |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats Martinez, Paula Felippe [UNESP] Heart failure Skeletal muscle NADPH oxidase Myocardial infarction N-acetylcysteine Reactive oxygen species |
title_short |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
title_full |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
title_fullStr |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
title_full_unstemmed |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
title_sort |
Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats |
author |
Martinez, Paula Felippe [UNESP] |
author_facet |
Martinez, Paula Felippe [UNESP] Bonomo, Camila [UNESP] Guizoni, Daniele Mendes [UNESP] Oliveira Junior, Silvio Assis [UNESP] Damatto, Ricardo Luiz [UNESP] Cezar, Marcelo Diarcadia Mariano [UNESP] Lima, Aline Regina Ruiz [UNESP] Pagan, Luana Urbano [UNESP] Seiva, Fabio Rodrigues Fernandes, Denise Castro Laurindo, Francisco Rafael Martins Novelli, Ethel Lourenzi Barbosa [UNESP] Matsubara, Luiz Shiguero [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina Politi [UNESP] |
author_role |
author |
author2 |
Bonomo, Camila [UNESP] Guizoni, Daniele Mendes [UNESP] Oliveira Junior, Silvio Assis [UNESP] Damatto, Ricardo Luiz [UNESP] Cezar, Marcelo Diarcadia Mariano [UNESP] Lima, Aline Regina Ruiz [UNESP] Pagan, Luana Urbano [UNESP] Seiva, Fabio Rodrigues Fernandes, Denise Castro Laurindo, Francisco Rafael Martins Novelli, Ethel Lourenzi Barbosa [UNESP] Matsubara, Luiz Shiguero [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina Politi [UNESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual do Norte do Paraná (UENP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Martinez, Paula Felippe [UNESP] Bonomo, Camila [UNESP] Guizoni, Daniele Mendes [UNESP] Oliveira Junior, Silvio Assis [UNESP] Damatto, Ricardo Luiz [UNESP] Cezar, Marcelo Diarcadia Mariano [UNESP] Lima, Aline Regina Ruiz [UNESP] Pagan, Luana Urbano [UNESP] Seiva, Fabio Rodrigues Fernandes, Denise Castro Laurindo, Francisco Rafael Martins Novelli, Ethel Lourenzi Barbosa [UNESP] Matsubara, Luiz Shiguero [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina Politi [UNESP] |
dc.subject.por.fl_str_mv |
Heart failure Skeletal muscle NADPH oxidase Myocardial infarction N-acetylcysteine Reactive oxygen species |
topic |
Heart failure Skeletal muscle NADPH oxidase Myocardial infarction N-acetylcysteine Reactive oxygen species |
description |
Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:08:47Z 2015-10-21T13:08:47Z 2015-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.karger.com/Article/FullText/369683 Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015. 1015-8987 http://hdl.handle.net/11449/128297 10.1159/000369683 WOS:000348048000014 WOS000348048000014.pdf 6309835137998766 1590971576309420 4463138671998432 5016839015394547 |
url |
http://www.karger.com/Article/FullText/369683 http://hdl.handle.net/11449/128297 |
identifier_str_mv |
Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015. 1015-8987 10.1159/000369683 WOS:000348048000014 WOS000348048000014.pdf 6309835137998766 1590971576309420 4463138671998432 5016839015394547 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology And Biochemistry 5.500 1,561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
148-159 application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128173228949504 |