Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats

Detalhes bibliográficos
Autor(a) principal: Martinez, Paula Felippe [UNESP]
Data de Publicação: 2015
Outros Autores: Bonomo, Camila [UNESP], Guizoni, Daniele Mendes [UNESP], Oliveira Junior, Silvio Assis [UNESP], Damatto, Ricardo Luiz [UNESP], Cezar, Marcelo Diarcadia Mariano [UNESP], Lima, Aline Regina Ruiz [UNESP], Pagan, Luana Urbano [UNESP], Seiva, Fabio Rodrigues, Fernandes, Denise Castro, Laurindo, Francisco Rafael Martins, Novelli, Ethel Lourenzi Barbosa [UNESP], Matsubara, Luiz Shiguero [UNESP], Zornoff, Leonardo Antonio Mamede [UNESP], Okoshi, Katashi [UNESP], Okoshi, Marina Politi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.karger.com/Article/FullText/369683
http://hdl.handle.net/11449/128297
Resumo: Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity.
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spelling Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure ratsHeart failureSkeletal muscleNADPH oxidaseMyocardial infarctionN-acetylcysteineReactive oxygen speciesBackground: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade de São Paulo, Instituto do Coração do Hospital das Clínicas, Faculdade de MedicinaUniversidade Estadual Paulista, Departamento de Clínica Médica, Faculdade de Medicina de BotucatuFAPESP: 2010/50461-6FAPESP: 2007/59500-1CNPq: 306857/2012-0CNPq: 306.845/2012-1KargerUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual do Norte do Paraná (UENP)Universidade de São Paulo (USP)Martinez, Paula Felippe [UNESP]Bonomo, Camila [UNESP]Guizoni, Daniele Mendes [UNESP]Oliveira Junior, Silvio Assis [UNESP]Damatto, Ricardo Luiz [UNESP]Cezar, Marcelo Diarcadia Mariano [UNESP]Lima, Aline Regina Ruiz [UNESP]Pagan, Luana Urbano [UNESP]Seiva, Fabio RodriguesFernandes, Denise CastroLaurindo, Francisco Rafael MartinsNovelli, Ethel Lourenzi Barbosa [UNESP]Matsubara, Luiz Shiguero [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Okoshi, Katashi [UNESP]Okoshi, Marina Politi [UNESP]2015-10-21T13:08:47Z2015-10-21T13:08:47Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article148-159application/pdfhttp://www.karger.com/Article/FullText/369683Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015.1015-8987http://hdl.handle.net/11449/12829710.1159/000369683WOS:000348048000014WOS000348048000014.pdf6309835137998766159097157630942044631386719984325016839015394547Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology And Biochemistry5.5001,561info:eu-repo/semantics/openAccess2024-08-14T17:23:43Zoai:repositorio.unesp.br:11449/128297Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
title Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
spellingShingle Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
Martinez, Paula Felippe [UNESP]
Heart failure
Skeletal muscle
NADPH oxidase
Myocardial infarction
N-acetylcysteine
Reactive oxygen species
title_short Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
title_full Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
title_fullStr Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
title_full_unstemmed Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
title_sort Influence of N-acetylcysteine on oxidative stress in slow-twitch soleus muscle of heart failure rats
author Martinez, Paula Felippe [UNESP]
author_facet Martinez, Paula Felippe [UNESP]
Bonomo, Camila [UNESP]
Guizoni, Daniele Mendes [UNESP]
Oliveira Junior, Silvio Assis [UNESP]
Damatto, Ricardo Luiz [UNESP]
Cezar, Marcelo Diarcadia Mariano [UNESP]
Lima, Aline Regina Ruiz [UNESP]
Pagan, Luana Urbano [UNESP]
Seiva, Fabio Rodrigues
Fernandes, Denise Castro
Laurindo, Francisco Rafael Martins
Novelli, Ethel Lourenzi Barbosa [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
author_role author
author2 Bonomo, Camila [UNESP]
Guizoni, Daniele Mendes [UNESP]
Oliveira Junior, Silvio Assis [UNESP]
Damatto, Ricardo Luiz [UNESP]
Cezar, Marcelo Diarcadia Mariano [UNESP]
Lima, Aline Regina Ruiz [UNESP]
Pagan, Luana Urbano [UNESP]
Seiva, Fabio Rodrigues
Fernandes, Denise Castro
Laurindo, Francisco Rafael Martins
Novelli, Ethel Lourenzi Barbosa [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso do Sul (UFMS)
Universidade Estadual do Norte do Paraná (UENP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Martinez, Paula Felippe [UNESP]
Bonomo, Camila [UNESP]
Guizoni, Daniele Mendes [UNESP]
Oliveira Junior, Silvio Assis [UNESP]
Damatto, Ricardo Luiz [UNESP]
Cezar, Marcelo Diarcadia Mariano [UNESP]
Lima, Aline Regina Ruiz [UNESP]
Pagan, Luana Urbano [UNESP]
Seiva, Fabio Rodrigues
Fernandes, Denise Castro
Laurindo, Francisco Rafael Martins
Novelli, Ethel Lourenzi Barbosa [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
dc.subject.por.fl_str_mv Heart failure
Skeletal muscle
NADPH oxidase
Myocardial infarction
N-acetylcysteine
Reactive oxygen species
topic Heart failure
Skeletal muscle
NADPH oxidase
Myocardial infarction
N-acetylcysteine
Reactive oxygen species
description Background: Chronic heart failure is characterized by decreased exercise capacity with early exacerbation of fatigue and dyspnea. Intrinsic skeletal muscle abnormalities can play a role in exercise intolerance. Causal or contributing factors responsible for muscle alterations have not been completely defined. This study evaluated skeletal muscle oxidative stress and NADPH oxidase activity in rats with myocardial infarction (MI) induced heart failure. Methods and Results: Four months after MI, rats were assigned to Sham, MI-C (without treatment), and MI-NAC (treated with N-acetylcysteine) groups. Two months later, echocardiogram showed left ventricular dysfunction in MI-C; NAC attenuated diastolic dysfunction. In soleus muscle, glutathione peroxidase and superoxide dismutase activity was decreased in MI-C and unchanged by NAC. 3-nitrotyrosine was similar in MI-C and Sham, and lower in MI-NAC than MI-C. Total reactive oxygen species (ROS) production was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products. The 2-hydroxyethidium (EOH)/DHE ratio did not differ between Sham and MI-C and was higher in MI-NAC. The ethidium/DHE ratio was higher in MI-C than Sham and unchanged by NAC. NADPH oxidase activity was similar in Sham and MI-C and lower in MI-NAC. Gene expression of p47(phox) was lower in MI-C than Sham. NAC decreased NOX4 and p22(phox) expression. Conclusions: We corroborate the case that oxidative stress is increased in skeletal muscle of heart failure rats and show for the first time that oxidative stress is not related to increased NADPH oxidase activity.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T13:08:47Z
2015-10-21T13:08:47Z
2015-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.karger.com/Article/FullText/369683
Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015.
1015-8987
http://hdl.handle.net/11449/128297
10.1159/000369683
WOS:000348048000014
WOS000348048000014.pdf
6309835137998766
1590971576309420
4463138671998432
5016839015394547
url http://www.karger.com/Article/FullText/369683
http://hdl.handle.net/11449/128297
identifier_str_mv Cellular Physiology And Biochemistry. Basel: Karger, v. 35, n. 1, p. 148-159, 2015.
1015-8987
10.1159/000369683
WOS:000348048000014
WOS000348048000014.pdf
6309835137998766
1590971576309420
4463138671998432
5016839015394547
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology And Biochemistry
5.500
1,561
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 148-159
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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