Six-week anaerobic training improves proteolytic profile of diabetic rats

Detalhes bibliográficos
Autor(a) principal: de Oliveira-Batista, Rogério [UNESP]
Data de Publicação: 2015
Outros Autores: Silva, Angelita, dos Passos, Kaique Marques Rodrigues, Nogueira, Rosa Maria Barilli, Seraphim, Patricia Monteiro [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/2359-3997000000114
http://hdl.handle.net/11449/173056
Resumo: Objective: To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats. Materials and methods: Four groups were established: DE (Diabetes Exercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05. Results: We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level ​of E2-14kDa gene expression. Conclusion: Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats.
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spelling Six-week anaerobic training improves proteolytic profile of diabetic ratsAnaerobic trainingDiabetes mellitusProteolysisSkeletal muscleUbiquitin-proteasomeObjective: To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats. Materials and methods: Four groups were established: DE (Diabetes Exercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05. Results: We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level ​of E2-14kDa gene expression. Conclusion: Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats.Departamento de Fisioterapia Faculdade de Ciências e Tecnologia (FCT) Universidade Estadual Paulista (Unesp)Departamento de Medicina Veterinária Universidade do Oeste Paulista (Unoeste)Departamento de Fisioterapia Faculdade de Ciências e Tecnologia (FCT) Universidade Estadual Paulista (Unesp)Universidade Estadual Paulista (Unesp)Universidade do Oeste Paulista (Unoeste)de Oliveira-Batista, Rogério [UNESP]Silva, Angelitados Passos, Kaique Marques RodriguesNogueira, Rosa Maria BarilliSeraphim, Patricia Monteiro [UNESP]2018-12-11T17:03:18Z2018-12-11T17:03:18Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article400-406application/pdfhttp://dx.doi.org/10.1590/2359-3997000000114Archives of Endocrinology and Metabolism, v. 59, n. 5, p. 400-406, 2015.2359-42922359-3997http://hdl.handle.net/11449/17305610.1590/2359-3997000000114S2359-399720150005004002-s2.0-84973427491S2359-39972015000500400.pdf04110085990708710000-0003-2145-6640Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Endocrinology and Metabolism0,5050,505info:eu-repo/semantics/openAccess2024-06-18T18:44:01Zoai:repositorio.unesp.br:11449/173056Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:10:46.126253Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Six-week anaerobic training improves proteolytic profile of diabetic rats
title Six-week anaerobic training improves proteolytic profile of diabetic rats
spellingShingle Six-week anaerobic training improves proteolytic profile of diabetic rats
de Oliveira-Batista, Rogério [UNESP]
Anaerobic training
Diabetes mellitus
Proteolysis
Skeletal muscle
Ubiquitin-proteasome
title_short Six-week anaerobic training improves proteolytic profile of diabetic rats
title_full Six-week anaerobic training improves proteolytic profile of diabetic rats
title_fullStr Six-week anaerobic training improves proteolytic profile of diabetic rats
title_full_unstemmed Six-week anaerobic training improves proteolytic profile of diabetic rats
title_sort Six-week anaerobic training improves proteolytic profile of diabetic rats
author de Oliveira-Batista, Rogério [UNESP]
author_facet de Oliveira-Batista, Rogério [UNESP]
Silva, Angelita
dos Passos, Kaique Marques Rodrigues
Nogueira, Rosa Maria Barilli
Seraphim, Patricia Monteiro [UNESP]
author_role author
author2 Silva, Angelita
dos Passos, Kaique Marques Rodrigues
Nogueira, Rosa Maria Barilli
Seraphim, Patricia Monteiro [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade do Oeste Paulista (Unoeste)
dc.contributor.author.fl_str_mv de Oliveira-Batista, Rogério [UNESP]
Silva, Angelita
dos Passos, Kaique Marques Rodrigues
Nogueira, Rosa Maria Barilli
Seraphim, Patricia Monteiro [UNESP]
dc.subject.por.fl_str_mv Anaerobic training
Diabetes mellitus
Proteolysis
Skeletal muscle
Ubiquitin-proteasome
topic Anaerobic training
Diabetes mellitus
Proteolysis
Skeletal muscle
Ubiquitin-proteasome
description Objective: To evaluate the effect of six-week anaerobic training on the mRNA expression of genes related to proteolysis Ubb (Ubiquitin), E2-14kDa, Trim63 (MuRF1 protein) and Nfkb1 in the skeletal muscle of diabetic rats. Materials and methods: Four groups were established: DE (Diabetes Exercised), DS (Diabetes Sedentary), CE (Control Exercised) and CS (Control Sedentary). The training consisted of 3 sets of 12 jumps in the liquid mean with load equivalent to 50% of BW for 6 weeks. Euthanasia occurred under ip anesthesia, and blood, adipose tissue and skeletal muscles were collected. Gene expression was quantified by RT-PCR in the gastrocnemius muscle. ANOVA one-way was used for comparison among groups, with post-hoc (Tukey) when necessary, considering p < 0.05. Results: We observed reduction in the body weight and adipose tissue in the diabetic groups. The muscle mass was reduced in DS, which could be reversed by training (DE). Although DS and DE have presented similar body weight, the training protocol in DE promoted reduction in the adipose tissue, and increase of muscle mass. Anaerobic training was efficient to reduce glycaemia only in the diabetic animals until 6 hours after the end of training. The Trim63 gene expression was increased in DS; decreased Ubb gene level was observed in trained rats (CE and DE) compared to sedentary (CS and DS), and DE presented the lowest level ​of E2-14kDa gene expression. Conclusion: Six-week anaerobic training promoted muscle mass gain, improved glycemic control, and exerted inhibitory effect on the proteolysis of gastrocnemius muscle of diabetic rats.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T17:03:18Z
2018-12-11T17:03:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/2359-3997000000114
Archives of Endocrinology and Metabolism, v. 59, n. 5, p. 400-406, 2015.
2359-4292
2359-3997
http://hdl.handle.net/11449/173056
10.1590/2359-3997000000114
S2359-39972015000500400
2-s2.0-84973427491
S2359-39972015000500400.pdf
0411008599070871
0000-0003-2145-6640
url http://dx.doi.org/10.1590/2359-3997000000114
http://hdl.handle.net/11449/173056
identifier_str_mv Archives of Endocrinology and Metabolism, v. 59, n. 5, p. 400-406, 2015.
2359-4292
2359-3997
10.1590/2359-3997000000114
S2359-39972015000500400
2-s2.0-84973427491
S2359-39972015000500400.pdf
0411008599070871
0000-0003-2145-6640
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Endocrinology and Metabolism
0,505
0,505
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 400-406
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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