Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0201882 http://hdl.handle.net/11449/177178 |
Resumo: | Whole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes. In Brazil, there is relatively few epidemiological data on Salmonella. In this study, 90 Salmonella Typhimurium strains had their genome sequenced to uncover the diversity of Salmonella Typhimurium isolated from humans and food, between 1983 and 2013, from different geographic regions in Brazil based on single nucleotide polymorphism (SNP) analysis. A total of 39 resistance genes were identified, such as aminoglycoside, tetracycline, sulfonamide, trimethoprim, beta-lactam, fluoroquinolone, phenicol and macrolide, as well as the occurrence of point mutations in some of the genes such as gyrA, gyrB, parC and parE. A total of 65 (72.2%) out of 90 S. Typhimurium strains studied were phenotypically resistant to sulfonamides, 44 (48.9%) strains were streptomycin resistant, 27 (30%) strains were resistant to tetracycline, 21 (23.3%) strains were gentamicin resistant, and seven (7.8%) strains were resistant to ceftriaxone. In the gyrA gene, it was observed the following amino acid substitutions: Asp(87)!Gly, Asp(87)! Asn, Ser(83)!Phe, Ser(83)!Tyr. Phylogenetic results placed the 90 S. Typhimurium strains into two major clades suggesting the existence of a prevalent subtype, likely more adapted, among strains isolated from humans, with some diversity in subtypes in foods. The variety and prevalence of resistant genes found in these Salmonella Typhimurium strains reinforces their potential hazard for humans and the risk in foods in Brazil. |
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Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencingWhole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes. In Brazil, there is relatively few epidemiological data on Salmonella. In this study, 90 Salmonella Typhimurium strains had their genome sequenced to uncover the diversity of Salmonella Typhimurium isolated from humans and food, between 1983 and 2013, from different geographic regions in Brazil based on single nucleotide polymorphism (SNP) analysis. A total of 39 resistance genes were identified, such as aminoglycoside, tetracycline, sulfonamide, trimethoprim, beta-lactam, fluoroquinolone, phenicol and macrolide, as well as the occurrence of point mutations in some of the genes such as gyrA, gyrB, parC and parE. A total of 65 (72.2%) out of 90 S. Typhimurium strains studied were phenotypically resistant to sulfonamides, 44 (48.9%) strains were streptomycin resistant, 27 (30%) strains were resistant to tetracycline, 21 (23.3%) strains were gentamicin resistant, and seven (7.8%) strains were resistant to ceftriaxone. In the gyrA gene, it was observed the following amino acid substitutions: Asp(87)!Gly, Asp(87)! Asn, Ser(83)!Phe, Ser(83)!Tyr. Phylogenetic results placed the 90 S. Typhimurium strains into two major clades suggesting the existence of a prevalent subtype, likely more adapted, among strains isolated from humans, with some diversity in subtypes in foods. The variety and prevalence of resistant genes found in these Salmonella Typhimurium strains reinforces their potential hazard for humans and the risk in foods in Brazil.Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, Av. do Café s/ nºCentro de Laboratório Regional de Ribeirão Preto—Instituto Adolfo Lutz, Rua MinasLaboratório de Enterobactérias FIOCRUZ Fundação Instituto Oswaldo Cruz, Avenida Brasil, Pavilhão Rocha Lima, 3andarFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)Division of Microbiology Office of Regular Science Center for Food Safety and Applied Nutrition U.S. Food and Drug AdministrationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Centro de Laboratório Regional de Ribeirão Preto—Instituto Adolfo LutzFundação Instituto Oswaldo CruzUniversidade Estadual Paulista (Unesp)U.S. Food and Drug AdministrationAlmeida, FernandaSeribelli, Amanda AparecidaCazentini Medeiros, Marta InêsRodrigues, Dália dos PrazeresDe MelloVarani, Alessandro [UNESP]Luo, YanAllard, Marc W.Falcão, Juliana Pfrimer2018-12-11T17:24:21Z2018-12-11T17:24:21Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1DUMMYapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0201882PLoS ONE, v. 13, n. 8, p. 1DUMMY-, 2018.1932-6203http://hdl.handle.net/11449/17717810.1371/journal.pone.02018822-s2.0-850515197142-s2.0-85051519714.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-06-24T14:51:30Zoai:repositorio.unesp.br:11449/177178Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T14:51:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
title |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
spellingShingle |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing Almeida, Fernanda |
title_short |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
title_full |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
title_fullStr |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
title_full_unstemmed |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
title_sort |
Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing |
author |
Almeida, Fernanda |
author_facet |
Almeida, Fernanda Seribelli, Amanda Aparecida Cazentini Medeiros, Marta Inês Rodrigues, Dália dos Prazeres De MelloVarani, Alessandro [UNESP] Luo, Yan Allard, Marc W. Falcão, Juliana Pfrimer |
author_role |
author |
author2 |
Seribelli, Amanda Aparecida Cazentini Medeiros, Marta Inês Rodrigues, Dália dos Prazeres De MelloVarani, Alessandro [UNESP] Luo, Yan Allard, Marc W. Falcão, Juliana Pfrimer |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Centro de Laboratório Regional de Ribeirão Preto—Instituto Adolfo Lutz Fundação Instituto Oswaldo Cruz Universidade Estadual Paulista (Unesp) U.S. Food and Drug Administration |
dc.contributor.author.fl_str_mv |
Almeida, Fernanda Seribelli, Amanda Aparecida Cazentini Medeiros, Marta Inês Rodrigues, Dália dos Prazeres De MelloVarani, Alessandro [UNESP] Luo, Yan Allard, Marc W. Falcão, Juliana Pfrimer |
description |
Whole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes. In Brazil, there is relatively few epidemiological data on Salmonella. In this study, 90 Salmonella Typhimurium strains had their genome sequenced to uncover the diversity of Salmonella Typhimurium isolated from humans and food, between 1983 and 2013, from different geographic regions in Brazil based on single nucleotide polymorphism (SNP) analysis. A total of 39 resistance genes were identified, such as aminoglycoside, tetracycline, sulfonamide, trimethoprim, beta-lactam, fluoroquinolone, phenicol and macrolide, as well as the occurrence of point mutations in some of the genes such as gyrA, gyrB, parC and parE. A total of 65 (72.2%) out of 90 S. Typhimurium strains studied were phenotypically resistant to sulfonamides, 44 (48.9%) strains were streptomycin resistant, 27 (30%) strains were resistant to tetracycline, 21 (23.3%) strains were gentamicin resistant, and seven (7.8%) strains were resistant to ceftriaxone. In the gyrA gene, it was observed the following amino acid substitutions: Asp(87)!Gly, Asp(87)! Asn, Ser(83)!Phe, Ser(83)!Tyr. Phylogenetic results placed the 90 S. Typhimurium strains into two major clades suggesting the existence of a prevalent subtype, likely more adapted, among strains isolated from humans, with some diversity in subtypes in foods. The variety and prevalence of resistant genes found in these Salmonella Typhimurium strains reinforces their potential hazard for humans and the risk in foods in Brazil. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:24:21Z 2018-12-11T17:24:21Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0201882 PLoS ONE, v. 13, n. 8, p. 1DUMMY-, 2018. 1932-6203 http://hdl.handle.net/11449/177178 10.1371/journal.pone.0201882 2-s2.0-85051519714 2-s2.0-85051519714.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0201882 http://hdl.handle.net/11449/177178 |
identifier_str_mv |
PLoS ONE, v. 13, n. 8, p. 1DUMMY-, 2018. 1932-6203 10.1371/journal.pone.0201882 2-s2.0-85051519714 2-s2.0-85051519714.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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PLoS ONE 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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1DUMMY application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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