Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing

Detalhes bibliográficos
Autor(a) principal: Almeida, Fernanda
Data de Publicação: 2018
Outros Autores: Seribelli, Amanda Aparecida, Medeiros, Marta Inês Cazentini, Rodrigues, Dália dos Prazeres, Varani, Alessandro de Mello, Luo, Yan, Allard, Marc W., Falcão, Juliana Pfrimer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31552
Resumo: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
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spelling Almeida, FernandaSeribelli, Amanda AparecidaMedeiros, Marta Inês CazentiniRodrigues, Dália dos PrazeresVarani, Alessandro de MelloLuo, YanAllard, Marc W.Falcão, Juliana Pfrimer2019-02-12T10:56:00Z2019-02-12T10:56:00Z2018ALMEIDA, Fernanda; et al. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. Plos One, v.13, n.8, e0201882, 16p, Aug. 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3155210.1371/journal.pone.0201882engPublic Library of ScienceSequenciamento Completo do GenomaCepasSalmonella TyphimuriumResistência antimicrobianaFilogenéticaAnálise genéticaBrasilSalmonella TyphimuriumStrainsWhole Genome SequencingAntimicrobial resistancePhylogeneticGene analysisBrazilPhylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Instituto Adolfo Lutz. Centro de Laboratório Regional de Ribeirão Preto. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ. Brasil.Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal, SP, Brasil..U.S. Food and Drug Administration. Center for Food Safety and Applied Nutrition. Division of Microbiology, Office of Regular Science. College Park, Maryland, USA.U.S. Food and Drug Administration. Center for Food Safety and Applied Nutrition. Division of Microbiology, Office of Regular Science. College Park, Maryland, USA.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Whole genome sequencing (WGS) has been used as a powerful technology for molecular epidemiology, surveillance, identification of species and serotype, identification of the sources of outbreaks, among other purposes. In Brazil, there is relatively few epidemiological data on Salmonella. In this study, 90 Salmonella Typhimurium strains had their genome sequenced to uncover the diversity of Salmonella Typhimurium isolated from humans and food, between 1983 and 2013, from different geographic regions in Brazil based on single nucleotide polymorphism (SNP) analysis. A total of 39 resistance genes were identified, such as aminoglycoside, tetracycline, sulfonamide, trimethoprim, beta-lactam, fluoroquinolone, phenicol and macrolide, as well as the occurrence of point mutations in some of the genes such as gyrA, gyrB, parC and parE. A total of 65 (72.2%) out of 90 S. Typhimurium strains studied were phenotypically resistant to sulfonamides, 44 (48.9%) strains were streptomycin resistant, 27 (30%) strains were resistant to tetracycline, 21 (23.3%) strains were gentamicin resistant, and seven (7.8%) strains were resistant to ceftriaxone. In the gyrA gene, it was observed the following amino acid substitutions: Asp(87)→Gly, Asp(87)→Asn, Ser(83)→Phe, Ser(83)→Tyr. Phylogenetic results placed the 90 S. Typhimurium strains into two major clades suggesting the existence of a prevalent subtype, likely more adapted, among strains isolated from humans, with some diversity in subtypes in foods. The variety and prevalence of resistant genes found in these Salmonella Typhimurium strains reinforces their potential hazard for humans and the risk in foods in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31552/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdaliaprazeres_rodrigues_etal_IOC_2018.pdfdaliaprazeres_rodrigues_etal_IOC_2018.pdfapplication/pdf2669595https://www.arca.fiocruz.br/bitstream/icict/31552/2/daliaprazeres_rodrigues_etal_IOC_2018.pdf16bd34017bb4d9764d10864f5600f6f1MD52TEXTdaliaprazeres_rodrigues_etal_IOC_2018.pdf.txtdaliaprazeres_rodrigues_etal_IOC_2018.pdf.txtExtracted texttext/plain61033https://www.arca.fiocruz.br/bitstream/icict/31552/3/daliaprazeres_rodrigues_etal_IOC_2018.pdf.txtd58ea4f553d5b2e5fa3e59a96060502dMD53icict/315522019-02-13 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dc.title.pt_BR.fl_str_mv Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
title Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
spellingShingle Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
Almeida, Fernanda
Sequenciamento Completo do Genoma
Cepas
Salmonella Typhimurium
Resistência antimicrobiana
Filogenética
Análise genética
Brasil
Salmonella Typhimurium
Strains
Whole Genome Sequencing
Antimicrobial resistance
Phylogenetic
Gene analysis
Brazil
title_short Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
title_full Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
title_fullStr Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
title_full_unstemmed Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
title_sort Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing
author Almeida, Fernanda
author_facet Almeida, Fernanda
Seribelli, Amanda Aparecida
Medeiros, Marta Inês Cazentini
Rodrigues, Dália dos Prazeres
Varani, Alessandro de Mello
Luo, Yan
Allard, Marc W.
Falcão, Juliana Pfrimer
author_role author
author2 Seribelli, Amanda Aparecida
Medeiros, Marta Inês Cazentini
Rodrigues, Dália dos Prazeres
Varani, Alessandro de Mello
Luo, Yan
Allard, Marc W.
Falcão, Juliana Pfrimer
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Fernanda
Seribelli, Amanda Aparecida
Medeiros, Marta Inês Cazentini
Rodrigues, Dália dos Prazeres
Varani, Alessandro de Mello
Luo, Yan
Allard, Marc W.
Falcão, Juliana Pfrimer
dc.subject.other.pt_BR.fl_str_mv Sequenciamento Completo do Genoma
Cepas
Salmonella Typhimurium
Resistência antimicrobiana
Filogenética
Análise genética
Brasil
topic Sequenciamento Completo do Genoma
Cepas
Salmonella Typhimurium
Resistência antimicrobiana
Filogenética
Análise genética
Brasil
Salmonella Typhimurium
Strains
Whole Genome Sequencing
Antimicrobial resistance
Phylogenetic
Gene analysis
Brazil
dc.subject.en.pt_BR.fl_str_mv Salmonella Typhimurium
Strains
Whole Genome Sequencing
Antimicrobial resistance
Phylogenetic
Gene analysis
Brazil
description Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-12T10:56:00Z
dc.date.available.fl_str_mv 2019-02-12T10:56:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Fernanda; et al. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. Plos One, v.13, n.8, e0201882, 16p, Aug. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31552
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0201882
identifier_str_mv ALMEIDA, Fernanda; et al. Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. Plos One, v.13, n.8, e0201882, 16p, Aug. 2018.
1932-6203
10.1371/journal.pone.0201882
url https://www.arca.fiocruz.br/handle/icict/31552
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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