Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fnut.2021.775515 http://hdl.handle.net/11449/230186 |
Resumo: | Introduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers. Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment. Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected. Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer. |
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Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover StudymicroRNAmolecular pathwaysorange juiceoxidative stresssmokersIntroduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers. Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment. Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected. Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Internal Medicine Department Botucatu Medical School São Paulo State University UNESPExperimental Research Unit São Paulo State University UNESPBotucatu Medical School São Paulo State University UNESPChemistry and Biochemistry Department Institute of Biosciences São Paulo State University UNESPDepartment of Nutrition School of Public Health USP - University of São PauloDepartment of Surgery and Orthopedics São Paulo State University UNESPInternal Medicine Department Botucatu Medical School São Paulo State University UNESPExperimental Research Unit São Paulo State University UNESPBotucatu Medical School São Paulo State University UNESPChemistry and Biochemistry Department Institute of Biosciences São Paulo State University UNESPDepartment of Surgery and Orthopedics São Paulo State University UNESPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Dorna, Mariana S. [UNESP]Barbosa, Elizabete M. S. [UNESP]Callegari, Matheus A. [UNESP]Tanni, Suzana E. [UNESP]Chiuso-Minicucci, Fernanda [UNESP]Felix, Tainara F. [UNESP]Seneda, Ana L. [UNESP]Correa, Camila R. [UNESP]Fernandes, Ana A. H. [UNESP]Azevedo, Paula S. [UNESP]Polegato, Bertha F. [UNESP]Rogero, Marcelo M.Paiva, Sergio A. R. [UNESP]Zornoff, Leonardo A. M. [UNESP]Reis, Patricia P. [UNESP]Minicucci, Marcos F. [UNESP]2022-04-29T08:38:18Z2022-04-29T08:38:18Z2021-12-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fnut.2021.775515Frontiers in Nutrition, v. 8.2296-861Xhttp://hdl.handle.net/11449/23018610.3389/fnut.2021.7755152-s2.0-85122486314Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Nutritioninfo:eu-repo/semantics/openAccess2024-08-14T17:36:54Zoai:repositorio.unesp.br:11449/230186Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
title |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
spellingShingle |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study Dorna, Mariana S. [UNESP] microRNA molecular pathways orange juice oxidative stress smokers |
title_short |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
title_full |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
title_fullStr |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
title_full_unstemmed |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
title_sort |
Orange Juice Attenuates Circulating miR-150-5p, miR-25-3p, and miR-451a in Healthy Smokers: A Randomized Crossover Study |
author |
Dorna, Mariana S. [UNESP] |
author_facet |
Dorna, Mariana S. [UNESP] Barbosa, Elizabete M. S. [UNESP] Callegari, Matheus A. [UNESP] Tanni, Suzana E. [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Felix, Tainara F. [UNESP] Seneda, Ana L. [UNESP] Correa, Camila R. [UNESP] Fernandes, Ana A. H. [UNESP] Azevedo, Paula S. [UNESP] Polegato, Bertha F. [UNESP] Rogero, Marcelo M. Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] Reis, Patricia P. [UNESP] Minicucci, Marcos F. [UNESP] |
author_role |
author |
author2 |
Barbosa, Elizabete M. S. [UNESP] Callegari, Matheus A. [UNESP] Tanni, Suzana E. [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Felix, Tainara F. [UNESP] Seneda, Ana L. [UNESP] Correa, Camila R. [UNESP] Fernandes, Ana A. H. [UNESP] Azevedo, Paula S. [UNESP] Polegato, Bertha F. [UNESP] Rogero, Marcelo M. Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] Reis, Patricia P. [UNESP] Minicucci, Marcos F. [UNESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dorna, Mariana S. [UNESP] Barbosa, Elizabete M. S. [UNESP] Callegari, Matheus A. [UNESP] Tanni, Suzana E. [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Felix, Tainara F. [UNESP] Seneda, Ana L. [UNESP] Correa, Camila R. [UNESP] Fernandes, Ana A. H. [UNESP] Azevedo, Paula S. [UNESP] Polegato, Bertha F. [UNESP] Rogero, Marcelo M. Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] Reis, Patricia P. [UNESP] Minicucci, Marcos F. [UNESP] |
dc.subject.por.fl_str_mv |
microRNA molecular pathways orange juice oxidative stress smokers |
topic |
microRNA molecular pathways orange juice oxidative stress smokers |
description |
Introduction: Tobacco smoke is associated with oxidative and inflammatory pathways, increasing the risk of chronic-degenerative diseases. Our goal was to evaluate the effects of acute “Pera” and “Moro” orange juice consumption on inflammatory processes and oxidative stress in microRNA (miRNA) expression in plasma from healthy smokers. Methods: This was a randomized crossover study that included healthy smokers over 18 years old. Blood samples were collected before and 11 h after beverage ingestion. Participants were instructed to drink 400 mL of Pera orange juice (Citrus sinensis), Moro orange juice (Citrus sinensis L. Osbeck), or water. Each subject drank the beverages in a 3-way crossover study design. Inflammatory and oxidative stress biomarkers and circulating miRNA expression profiles were determined. The subjects maintained their usual tobacco exposure during the experiment. Results: We included 18 individuals (12 men and 6 women), with 37.0 ± 12.0 years old. All subjects received the 3 interventions. Increased expression of circulating miRNAs (miR-150-5p, miR-25-3p, and miR-451a) was verified after cigarette smoking, which were attenuated after intake of both types of orange juice. There was no difference regarding serum levels of TNF-α, IL-6, MMP-9, and C-reactive protein. Despite the increased activity of serum superoxide dismutase and glutathione peroxidase after “Pera” or “Moro” orange juice intake, respectively, no changes in lipid hydroperoxide levels were detected. Conclusion: Tobaccos smokers showed increased expression of miR-150-5p, miR-25-3p, and miR-451a was noted, and attenuated by orange juice intake. miRNAs were predicted to regulate 244 target genes with roles in oxidative stress, PI3K-Akt, and MAPK signaling, which are pathways frequently involved in smoking-related cardiovascular diseases and cancer. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-24 2022-04-29T08:38:18Z 2022-04-29T08:38:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fnut.2021.775515 Frontiers in Nutrition, v. 8. 2296-861X http://hdl.handle.net/11449/230186 10.3389/fnut.2021.775515 2-s2.0-85122486314 |
url |
http://dx.doi.org/10.3389/fnut.2021.775515 http://hdl.handle.net/11449/230186 |
identifier_str_mv |
Frontiers in Nutrition, v. 8. 2296-861X 10.3389/fnut.2021.775515 2-s2.0-85122486314 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Nutrition |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128209426841600 |