Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biortech.2018.06.091 http://hdl.handle.net/11449/176657 |
Resumo: | The effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus. |
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Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rateAnaerobic digestionBiogasMetagenomics of anaerobic sludgeMethanogenic archaeaPollutant removalThe effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Post-Graduate Program in Agricultural and Livestock Microbiology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Environmental Sanitation Department of Rural Engineering São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Biochemistry and Molecular Biology Department of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalFaculty of Technology, JaboticabalEmbrapa Informática Agropecuária, CampinasPost-Graduate Program in Agricultural and Livestock Microbiology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Environmental Sanitation Department of Rural Engineering São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Biochemistry and Molecular Biology Department of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalUniversidade Estadual Paulista (Unesp)Faculty of TechnologyEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Botello Suárez, Wilmar Alirio [UNESP]da Silva Vantini, Juliana [UNESP]Duda, Rose MariaGiachetto, Poliana FernandaCintra, Leandro CarrijoTiraboschi Ferro, Maria Inês [UNESP]de Oliveira, Roberto Alves [UNESP]2018-12-11T17:21:57Z2018-12-11T17:21:57Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article158-168application/pdfhttp://dx.doi.org/10.1016/j.biortech.2018.06.091Bioresource Technology, v. 268, p. 158-168.1873-29760960-8524http://hdl.handle.net/11449/17665710.1016/j.biortech.2018.06.0912-s2.0-850507995982-s2.0-85050799598.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioresource Technology2,029info:eu-repo/semantics/openAccess2024-06-07T15:32:36Zoai:repositorio.unesp.br:11449/176657Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:45:10.138205Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
title |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
spellingShingle |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate Botello Suárez, Wilmar Alirio [UNESP] Anaerobic digestion Biogas Metagenomics of anaerobic sludge Methanogenic archaea Pollutant removal |
title_short |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
title_full |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
title_fullStr |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
title_full_unstemmed |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
title_sort |
Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate |
author |
Botello Suárez, Wilmar Alirio [UNESP] |
author_facet |
Botello Suárez, Wilmar Alirio [UNESP] da Silva Vantini, Juliana [UNESP] Duda, Rose Maria Giachetto, Poliana Fernanda Cintra, Leandro Carrijo Tiraboschi Ferro, Maria Inês [UNESP] de Oliveira, Roberto Alves [UNESP] |
author_role |
author |
author2 |
da Silva Vantini, Juliana [UNESP] Duda, Rose Maria Giachetto, Poliana Fernanda Cintra, Leandro Carrijo Tiraboschi Ferro, Maria Inês [UNESP] de Oliveira, Roberto Alves [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculty of Technology Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Botello Suárez, Wilmar Alirio [UNESP] da Silva Vantini, Juliana [UNESP] Duda, Rose Maria Giachetto, Poliana Fernanda Cintra, Leandro Carrijo Tiraboschi Ferro, Maria Inês [UNESP] de Oliveira, Roberto Alves [UNESP] |
dc.subject.por.fl_str_mv |
Anaerobic digestion Biogas Metagenomics of anaerobic sludge Methanogenic archaea Pollutant removal |
topic |
Anaerobic digestion Biogas Metagenomics of anaerobic sludge Methanogenic archaea Pollutant removal |
description |
The effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:57Z 2018-12-11T17:21:57Z 2018-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biortech.2018.06.091 Bioresource Technology, v. 268, p. 158-168. 1873-2976 0960-8524 http://hdl.handle.net/11449/176657 10.1016/j.biortech.2018.06.091 2-s2.0-85050799598 2-s2.0-85050799598.pdf |
url |
http://dx.doi.org/10.1016/j.biortech.2018.06.091 http://hdl.handle.net/11449/176657 |
identifier_str_mv |
Bioresource Technology, v. 268, p. 158-168. 1873-2976 0960-8524 10.1016/j.biortech.2018.06.091 2-s2.0-85050799598 2-s2.0-85050799598.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioresource Technology 2,029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
158-168 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129458162368512 |