Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis

Detalhes bibliográficos
Autor(a) principal: Vieira, Gabriela Alves Licursi [UNESP]
Data de Publicação: 2021
Outros Autores: Cabral, Lucélia [UNESP], Otero, Igor Vinicius Ramos [UNESP], Ferro, Milene [UNESP], Faria, Adriano Uemura de [UNESP], Oliveira, Valéria Maia de, Bacci, Mauricio [UNESP], Sette, Lara Durães [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.chemosphere.2020.129190
http://hdl.handle.net/11449/206978
Resumo: The combination of different microorganisms and their metabolisms makes the use of microbial consortia in bioremediation processes a useful approach. In this sense, this study aimed at structuring and selecting a marine microbial consortium for Remazol Brilliant Blue R (RBBR) detoxification and decolorization. Experimental design was applied to improve the culture conditions, and metatranscriptomic analysis to understand the enzymatic pathways. A promising consortium composed of Mucor racemosus CBMAI 847, Marasmiellus sp. CBMAI 1062, Bacillus subtilis CBMAI 707, and Dietzia maris CBMAI 705 was selected. This consortium showed 52% of detoxification and 86% of decolorization in the validation assays after seven days of incubation in the presence of 500 ppm of RBBR. Reduction in RBBR color and toxicity were achieved by biosorption and microbial metabolisms. Metatranscriptomic data indicate that the consortium was able to decolorize and breakdown the RBBR molecule using a coordinated action of oxidases, oxygenases, and hydrolases. Epoxide hydrolases and glyoxalases expression could be associated with the decrease in toxicity. The efficiency of this marine microbial consortium suggests their use in bioremediation processes of textile effluents.
id UNSP_68f186ea4a16fabdaf20823069cdfd99
oai_identifier_str oai:repositorio.unesp.br:11449/206978
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysisBiodegradationBiosorptionExperimental designMarine fungiMetatranscriptomePetroleum associated bacteriaThe combination of different microorganisms and their metabolisms makes the use of microbial consortia in bioremediation processes a useful approach. In this sense, this study aimed at structuring and selecting a marine microbial consortium for Remazol Brilliant Blue R (RBBR) detoxification and decolorization. Experimental design was applied to improve the culture conditions, and metatranscriptomic analysis to understand the enzymatic pathways. A promising consortium composed of Mucor racemosus CBMAI 847, Marasmiellus sp. CBMAI 1062, Bacillus subtilis CBMAI 707, and Dietzia maris CBMAI 705 was selected. This consortium showed 52% of detoxification and 86% of decolorization in the validation assays after seven days of incubation in the presence of 500 ppm of RBBR. Reduction in RBBR color and toxicity were achieved by biosorption and microbial metabolisms. Metatranscriptomic data indicate that the consortium was able to decolorize and breakdown the RBBR molecule using a coordinated action of oxidases, oxygenases, and hydrolases. Epoxide hydrolases and glyoxalases expression could be associated with the decrease in toxicity. The efficiency of this marine microbial consortium suggests their use in bioremediation processes of textile effluents.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Geral e Aplicada Instituto de Biociências Universidade Estadual Paulista (UNESP)Centro de Estudos de Insetos Sociais Instituto de Biociências Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP) Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas Divisão de Recursos MicrobianosDepartamento de Biologia Geral e Aplicada Instituto de Biociências Universidade Estadual Paulista (UNESP)Centro de Estudos de Insetos Sociais Instituto de Biociências Universidade Estadual Paulista (UNESP)CNPq: 170714/2017–9FAPESP: 2012/12622–3FAPESP: 2013/19486–0FAPESP: 2016/07957–7FAPESP: 2018/03372–0FAPESP: 2018/12098–9CNPq: 303218/2019–3Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Vieira, Gabriela Alves Licursi [UNESP]Cabral, Lucélia [UNESP]Otero, Igor Vinicius Ramos [UNESP]Ferro, Milene [UNESP]Faria, Adriano Uemura de [UNESP]Oliveira, Valéria Maia deBacci, Mauricio [UNESP]Sette, Lara Durães [UNESP]2021-06-25T10:47:04Z2021-06-25T10:47:04Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2020.129190Chemosphere, v. 267.1879-12980045-6535http://hdl.handle.net/11449/20697810.1016/j.chemosphere.2020.1291902-s2.0-85097665431Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-04-11T14:57:29Zoai:repositorio.unesp.br:11449/206978Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:54:08.383763Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
title Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
spellingShingle Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
Vieira, Gabriela Alves Licursi [UNESP]
Biodegradation
Biosorption
Experimental design
Marine fungi
Metatranscriptome
Petroleum associated bacteria
title_short Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
title_full Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
title_fullStr Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
title_full_unstemmed Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
title_sort Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis
author Vieira, Gabriela Alves Licursi [UNESP]
author_facet Vieira, Gabriela Alves Licursi [UNESP]
Cabral, Lucélia [UNESP]
Otero, Igor Vinicius Ramos [UNESP]
Ferro, Milene [UNESP]
Faria, Adriano Uemura de [UNESP]
Oliveira, Valéria Maia de
Bacci, Mauricio [UNESP]
Sette, Lara Durães [UNESP]
author_role author
author2 Cabral, Lucélia [UNESP]
Otero, Igor Vinicius Ramos [UNESP]
Ferro, Milene [UNESP]
Faria, Adriano Uemura de [UNESP]
Oliveira, Valéria Maia de
Bacci, Mauricio [UNESP]
Sette, Lara Durães [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Vieira, Gabriela Alves Licursi [UNESP]
Cabral, Lucélia [UNESP]
Otero, Igor Vinicius Ramos [UNESP]
Ferro, Milene [UNESP]
Faria, Adriano Uemura de [UNESP]
Oliveira, Valéria Maia de
Bacci, Mauricio [UNESP]
Sette, Lara Durães [UNESP]
dc.subject.por.fl_str_mv Biodegradation
Biosorption
Experimental design
Marine fungi
Metatranscriptome
Petroleum associated bacteria
topic Biodegradation
Biosorption
Experimental design
Marine fungi
Metatranscriptome
Petroleum associated bacteria
description The combination of different microorganisms and their metabolisms makes the use of microbial consortia in bioremediation processes a useful approach. In this sense, this study aimed at structuring and selecting a marine microbial consortium for Remazol Brilliant Blue R (RBBR) detoxification and decolorization. Experimental design was applied to improve the culture conditions, and metatranscriptomic analysis to understand the enzymatic pathways. A promising consortium composed of Mucor racemosus CBMAI 847, Marasmiellus sp. CBMAI 1062, Bacillus subtilis CBMAI 707, and Dietzia maris CBMAI 705 was selected. This consortium showed 52% of detoxification and 86% of decolorization in the validation assays after seven days of incubation in the presence of 500 ppm of RBBR. Reduction in RBBR color and toxicity were achieved by biosorption and microbial metabolisms. Metatranscriptomic data indicate that the consortium was able to decolorize and breakdown the RBBR molecule using a coordinated action of oxidases, oxygenases, and hydrolases. Epoxide hydrolases and glyoxalases expression could be associated with the decrease in toxicity. The efficiency of this marine microbial consortium suggests their use in bioremediation processes of textile effluents.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:47:04Z
2021-06-25T10:47:04Z
2021-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.chemosphere.2020.129190
Chemosphere, v. 267.
1879-1298
0045-6535
http://hdl.handle.net/11449/206978
10.1016/j.chemosphere.2020.129190
2-s2.0-85097665431
url http://dx.doi.org/10.1016/j.chemosphere.2020.129190
http://hdl.handle.net/11449/206978
identifier_str_mv Chemosphere, v. 267.
1879-1298
0045-6535
10.1016/j.chemosphere.2020.129190
2-s2.0-85097665431
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosphere
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129471714164736