A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aca.2017.01.002 http://hdl.handle.net/11449/169352 |
Resumo: | This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (μPADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct μPADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D μPADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera. Both μPADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 μM, and 2.34 μM for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 μM, and 4.35 μM. |
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A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysisColorimetric detectionLow-cost diagnosticsPaper-based analytical devicesPoint-of-care devicesThis paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (μPADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct μPADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D μPADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera. Both μPADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 μM, and 2.34 μM for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 μM, and 4.35 μM.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Química Universidade Federal de São CarlosIntituto de Química-UNESP, Av. Francisco Degni s/nIntituto de Química-UNESP, Av. Francisco Degni s/nCNPq: 140484/2013-2FAPESP: 2015/19890-1CNPq: 427727/2016-2Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)de Oliveira, Ricardo A.G.Camargo, FiammaPesquero, Naira C. [UNESP]Faria, Ronaldo Censi2018-12-11T16:45:29Z2018-12-11T16:45:29Z2017-03-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-46application/pdfhttp://dx.doi.org/10.1016/j.aca.2017.01.002Analytica Chimica Acta, v. 957, p. 40-46.1873-43240003-2670http://hdl.handle.net/11449/16935210.1016/j.aca.2017.01.0022-s2.0-850094612402-s2.0-85009461240.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytica Chimica Acta1,512info:eu-repo/semantics/openAccess2023-11-12T06:07:36Zoai:repositorio.unesp.br:11449/169352Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:25:27.321603Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
title |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
spellingShingle |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis de Oliveira, Ricardo A.G. Colorimetric detection Low-cost diagnostics Paper-based analytical devices Point-of-care devices |
title_short |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
title_full |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
title_fullStr |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
title_full_unstemmed |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
title_sort |
A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis |
author |
de Oliveira, Ricardo A.G. |
author_facet |
de Oliveira, Ricardo A.G. Camargo, Fiamma Pesquero, Naira C. [UNESP] Faria, Ronaldo Censi |
author_role |
author |
author2 |
Camargo, Fiamma Pesquero, Naira C. [UNESP] Faria, Ronaldo Censi |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Oliveira, Ricardo A.G. Camargo, Fiamma Pesquero, Naira C. [UNESP] Faria, Ronaldo Censi |
dc.subject.por.fl_str_mv |
Colorimetric detection Low-cost diagnostics Paper-based analytical devices Point-of-care devices |
topic |
Colorimetric detection Low-cost diagnostics Paper-based analytical devices Point-of-care devices |
description |
This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (μPADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct μPADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D μPADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera. Both μPADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 μM, and 2.34 μM for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 μM, and 4.35 μM. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-08 2018-12-11T16:45:29Z 2018-12-11T16:45:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aca.2017.01.002 Analytica Chimica Acta, v. 957, p. 40-46. 1873-4324 0003-2670 http://hdl.handle.net/11449/169352 10.1016/j.aca.2017.01.002 2-s2.0-85009461240 2-s2.0-85009461240.pdf |
url |
http://dx.doi.org/10.1016/j.aca.2017.01.002 http://hdl.handle.net/11449/169352 |
identifier_str_mv |
Analytica Chimica Acta, v. 957, p. 40-46. 1873-4324 0003-2670 10.1016/j.aca.2017.01.002 2-s2.0-85009461240 2-s2.0-85009461240.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytica Chimica Acta 1,512 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40-46 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128809016229888 |