Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2018-0550 http://hdl.handle.net/11449/189527 |
Resumo: | PURPOSE: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). METHODOLOGY: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). RESULTS: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. CONCLUSION: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA. |
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spelling |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfatePURPOSE: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). METHODOLOGY: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). RESULTS: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. CONCLUSION: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.Universidade Federal de Alfenas (UNIFAL-MG) Faculdade de Odontologia Departamento de Clínica e CirurgiaUniversidade do Estado de São Paulo (UNESP) Faculdade de Odontologia de Araçatuba Departamento de EndodontiaUniversidade de São Paulo Departamento de Ciências Biológicas, Faculdade de Odontologia de BauruUniversidade Estadual de Londrina Departamento de Medicina Bucal e OdontopediatriaUniversidade de São Paulo Departamento de Odontopediatria Ortodontia e Saúde Coletiva, Faculdade de Odontologia de BauruUniversidade Federal de Alfenas (UNIFAL-MG) Instituto de Exatas Departamento de EstatísticaUniversidade Federal de Alfenas (UNIFAL-MG) Departamento de Biologia EstruturalUniversidade do Estado de São Paulo (UNESP) Faculdade de Odontologia de Araçatuba Departamento de EndodontiaFaculdade de OdontologiaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Estadual de Londrina (UEL)Instituto de ExatasUniversidade Federal de Alfenas (UNIFAL-MG)Lopes, Camila SoaresJunqueira, Marina AzevedoCosme-Silva, Leopoldo [UNESP]Pegoraro, Camila de Oliveira RodiniGarbelini, Cássia Cilene DezanOliveira, Thais MarchiniMartins, Natália SilvaNeves, Juliana Dos SantosSakai, Vivien Thiemy2019-10-06T16:43:34Z2019-10-06T16:43:34Z2019-07-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee20180550application/pdfhttp://dx.doi.org/10.1590/1678-7757-2018-0550Journal of applied oral science : revista FOB, v. 27, p. e20180550-.1678-7765http://hdl.handle.net/11449/18952710.1590/1678-7757-2018-0550S1678-775720190001004652-s2.0-85070914513S1678-77572019000100465.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of applied oral science : revista FOBinfo:eu-repo/semantics/openAccess2023-12-28T06:18:35Zoai:repositorio.unesp.br:11449/189527Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:30.046809Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
spellingShingle |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate Lopes, Camila Soares |
title_short |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_full |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_fullStr |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_full_unstemmed |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
title_sort |
Initial inflammatory response after the pulpotomy of rat molars with MTA or ferric sulfate |
author |
Lopes, Camila Soares |
author_facet |
Lopes, Camila Soares Junqueira, Marina Azevedo Cosme-Silva, Leopoldo [UNESP] Pegoraro, Camila de Oliveira Rodini Garbelini, Cássia Cilene Dezan Oliveira, Thais Marchini Martins, Natália Silva Neves, Juliana Dos Santos Sakai, Vivien Thiemy |
author_role |
author |
author2 |
Junqueira, Marina Azevedo Cosme-Silva, Leopoldo [UNESP] Pegoraro, Camila de Oliveira Rodini Garbelini, Cássia Cilene Dezan Oliveira, Thais Marchini Martins, Natália Silva Neves, Juliana Dos Santos Sakai, Vivien Thiemy |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Odontologia Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Estadual de Londrina (UEL) Instituto de Exatas Universidade Federal de Alfenas (UNIFAL-MG) |
dc.contributor.author.fl_str_mv |
Lopes, Camila Soares Junqueira, Marina Azevedo Cosme-Silva, Leopoldo [UNESP] Pegoraro, Camila de Oliveira Rodini Garbelini, Cássia Cilene Dezan Oliveira, Thais Marchini Martins, Natália Silva Neves, Juliana Dos Santos Sakai, Vivien Thiemy |
description |
PURPOSE: To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). METHODOLOGY: Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). RESULTS: The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. CONCLUSION: The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:43:34Z 2019-10-06T16:43:34Z 2019-07-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2018-0550 Journal of applied oral science : revista FOB, v. 27, p. e20180550-. 1678-7765 http://hdl.handle.net/11449/189527 10.1590/1678-7757-2018-0550 S1678-77572019000100465 2-s2.0-85070914513 S1678-77572019000100465.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2018-0550 http://hdl.handle.net/11449/189527 |
identifier_str_mv |
Journal of applied oral science : revista FOB, v. 27, p. e20180550-. 1678-7765 10.1590/1678-7757-2018-0550 S1678-77572019000100465 2-s2.0-85070914513 S1678-77572019000100465.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of applied oral science : revista FOB |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e20180550 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129330249728000 |