A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions

Detalhes bibliográficos
Autor(a) principal: Oliveira, Lucas Pires Garcia [UNESP]
Data de Publicação: 2019
Outros Autores: Conte, Fernanda Lopes [UNESP], de Oliveira Cardoso, Eliza [UNESP], Conti, Bruno José [UNESP], Santiago, Karina Basso [UNESP], de Assis Golim, Marjorie [UNESP], da Silva Feltran, Geórgia [UNESP], Zambuzzi, Willian Fernando [UNESP], Sforcin, José Maurício [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.lfs.2018.11.060
http://hdl.handle.net/11449/189946
Resumo: Aims: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. Main methods: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α IL-1β IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H 2 O 2 ) production were analyzed. Key findings: Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-α and IL-10 production, TLR-4 and CD80 expression, NF-κB and autophagy pathway, as well as the bactericidal activity. Significance: Our findings revealed a new chemotherapeutic approach using doxorubicin simultaneously with geopropolis without affecting human monocytes viability and exerting immunomodulatory effects, favoring cell functions. While doxorubicin altered some immunological parameters, the addition of geopropolis compensated some changes.
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spelling A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functionsChemotherapyGeopropolisImmunomodulationMonocytesAims: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. Main methods: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α IL-1β IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H 2 O 2 ) production were analyzed. Key findings: Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-α and IL-10 production, TLR-4 and CD80 expression, NF-κB and autophagy pathway, as well as the bactericidal activity. Significance: Our findings revealed a new chemotherapeutic approach using doxorubicin simultaneously with geopropolis without affecting human monocytes viability and exerting immunomodulatory effects, favoring cell functions. While doxorubicin altered some immunological parameters, the addition of geopropolis compensated some changes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Institute of BiosciencesBotucatu Blood Center School of Medicine UNESPSão Paulo State University (UNESP) Institute of BiosciencesBotucatu Blood Center School of Medicine UNESPFAPESP: 2016/02249-4Universidade Estadual Paulista (Unesp)Oliveira, Lucas Pires Garcia [UNESP]Conte, Fernanda Lopes [UNESP]de Oliveira Cardoso, Eliza [UNESP]Conti, Bruno José [UNESP]Santiago, Karina Basso [UNESP]de Assis Golim, Marjorie [UNESP]da Silva Feltran, Geórgia [UNESP]Zambuzzi, Willian Fernando [UNESP]Sforcin, José Maurício [UNESP]2019-10-06T16:57:23Z2019-10-06T16:57:23Z2019-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article81-90http://dx.doi.org/10.1016/j.lfs.2018.11.060Life Sciences, v. 217, p. 81-90.1879-06310024-3205http://hdl.handle.net/11449/18994610.1016/j.lfs.2018.11.0602-s2.0-85057869762Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2021-10-22T21:16:05Zoai:repositorio.unesp.br:11449/189946Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:16:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
title A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
spellingShingle A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
Oliveira, Lucas Pires Garcia [UNESP]
Chemotherapy
Geopropolis
Immunomodulation
Monocytes
title_short A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
title_full A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
title_fullStr A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
title_full_unstemmed A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
title_sort A new chemotherapeutic approach using doxorubicin simultaneously with geopropolis favoring monocyte functions
author Oliveira, Lucas Pires Garcia [UNESP]
author_facet Oliveira, Lucas Pires Garcia [UNESP]
Conte, Fernanda Lopes [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Conti, Bruno José [UNESP]
Santiago, Karina Basso [UNESP]
de Assis Golim, Marjorie [UNESP]
da Silva Feltran, Geórgia [UNESP]
Zambuzzi, Willian Fernando [UNESP]
Sforcin, José Maurício [UNESP]
author_role author
author2 Conte, Fernanda Lopes [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Conti, Bruno José [UNESP]
Santiago, Karina Basso [UNESP]
de Assis Golim, Marjorie [UNESP]
da Silva Feltran, Geórgia [UNESP]
Zambuzzi, Willian Fernando [UNESP]
Sforcin, José Maurício [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, Lucas Pires Garcia [UNESP]
Conte, Fernanda Lopes [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Conti, Bruno José [UNESP]
Santiago, Karina Basso [UNESP]
de Assis Golim, Marjorie [UNESP]
da Silva Feltran, Geórgia [UNESP]
Zambuzzi, Willian Fernando [UNESP]
Sforcin, José Maurício [UNESP]
dc.subject.por.fl_str_mv Chemotherapy
Geopropolis
Immunomodulation
Monocytes
topic Chemotherapy
Geopropolis
Immunomodulation
Monocytes
description Aims: Chemotherapy has been widely used to treat cancer although it may affect non-target cells involved in the immune response. This work aimed at elucidating whether the chemotherapeutic agent doxorubicin in combination with geopropolis produced by Melipona fasciculata Smith could affect nontumor immune cells, evaluating their immunomodulatory effects on human monocytes. Main methods: Cell viability, expression of cell markers (HLA-DR, TLR-2, TLR-4, C80 and CD40), cytokine production (TNF-α IL-1β IL-6 and IL-10), intracellular pathways (NF-κB and autophagy), the microbicidal activity of monocytes and hydrogen peroxide (H 2 O 2 ) production were analyzed. Key findings: Data showed that doxorubicin + geopropolis diminished IL-6 secretion, stimulated TNF-α and IL-10 production, TLR-4 and CD80 expression, NF-κB and autophagy pathway, as well as the bactericidal activity. Significance: Our findings revealed a new chemotherapeutic approach using doxorubicin simultaneously with geopropolis without affecting human monocytes viability and exerting immunomodulatory effects, favoring cell functions. While doxorubicin altered some immunological parameters, the addition of geopropolis compensated some changes.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:57:23Z
2019-10-06T16:57:23Z
2019-01-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.lfs.2018.11.060
Life Sciences, v. 217, p. 81-90.
1879-0631
0024-3205
http://hdl.handle.net/11449/189946
10.1016/j.lfs.2018.11.060
2-s2.0-85057869762
url http://dx.doi.org/10.1016/j.lfs.2018.11.060
http://hdl.handle.net/11449/189946
identifier_str_mv Life Sciences, v. 217, p. 81-90.
1879-0631
0024-3205
10.1016/j.lfs.2018.11.060
2-s2.0-85057869762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 81-90
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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