Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2017.03.056 http://hdl.handle.net/11449/163004 |
Resumo: | In this study, an evaluation was performed to determine the in vitro bioactivity, viability of stem cells, and antibiofilm effect against Streptococcus mutans of two bioactive gel-glass 60SiO(2)-36CaO-4P(2)O(5) (BG-A) and 80SiO(2)-15CaO-5P(2)O(5) (BG-B) compositions. Both materials were bioactive and undergo the formation of hydroxycarbonate apatite (HCA) on their surfaces when immersed in simulated body fluid (SBF) after 12 h, but the BG-A composition showed a more significant formation rate. The pH variation of the samples during the test in SBF indicated that an abrupt change had occurred for the BG-A composition within the first few hours, and the pH was subsequently maintained over time, supporting its stronger antibacterial effects against S. mutans. For the in vitro viability test using mesenchymal stem cells (MSCs), the BG-B showed significantly higher cell viability compared to the BG-A composition at concentrations of 0.125, 1.25 and 12.50 mg/mL for 2 days. These results indicated that the higher solubility of the BG-A glass favors bioactivity and antibacterial effects. However, as a result of rapid degradation, the increase in the concentration of ions in the cell culture medium was not favorable for cell proliferation. Thus, by varying the composition of glasses, and consequently their dissolution rate, it is possible to favor bioactivity, antimicrobial activity or stem cell proliferation for a particular application of interest. (C) 2017 Elsevier B.V. All rights reserved. |
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Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutansBioactive glassSol-gelIn vitro bioactivityCell viabilityStem cellsBiofilmIn this study, an evaluation was performed to determine the in vitro bioactivity, viability of stem cells, and antibiofilm effect against Streptococcus mutans of two bioactive gel-glass 60SiO(2)-36CaO-4P(2)O(5) (BG-A) and 80SiO(2)-15CaO-5P(2)O(5) (BG-B) compositions. Both materials were bioactive and undergo the formation of hydroxycarbonate apatite (HCA) on their surfaces when immersed in simulated body fluid (SBF) after 12 h, but the BG-A composition showed a more significant formation rate. The pH variation of the samples during the test in SBF indicated that an abrupt change had occurred for the BG-A composition within the first few hours, and the pH was subsequently maintained over time, supporting its stronger antibacterial effects against S. mutans. For the in vitro viability test using mesenchymal stem cells (MSCs), the BG-B showed significantly higher cell viability compared to the BG-A composition at concentrations of 0.125, 1.25 and 12.50 mg/mL for 2 days. These results indicated that the higher solubility of the BG-A glass favors bioactivity and antibacterial effects. However, as a result of rapid degradation, the increase in the concentration of ions in the cell culture medium was not favorable for cell proliferation. Thus, by varying the composition of glasses, and consequently their dissolution rate, it is possible to favor bioactivity, antimicrobial activity or stem cell proliferation for a particular application of interest. (C) 2017 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Carlos, Dept Engn Mat, Lab Mat Vitreos, Sao Carlos, SP, BrazilUniv Fed Rio Grande do Sul, Lab Hematol & Celulas Tronco, Fac Farm, Porto Alegre, RS, BrazilUniv Estadual Paulista, Dept Odontol Restauradora, Fac Odontol, Araraquara, SP, BrazilInst Pesquisa Celulas Tronco, Porto Alegre, RS, BrazilCtr Res Technol & Educ Vitreous Mat CeRTEV, Sao Carlos, SP, BrazilUniv Estadual Paulista, Dept Odontol Restauradora, Fac Odontol, Araraquara, SP, BrazilFAPESP: 2013/07793-6CNPq: 140516/2013-1Elsevier B.V.Universidade Federal de São Carlos (UFSCar)Univ Fed Rio Grande do SulUniversidade Estadual Paulista (Unesp)Inst Pesquisa Celulas TroncoCtr Res Technol & Educ Vitreous Mat CeRTEVSiqueira, Renato L.Maurmann, NatashaBurguez, DanielaPereira, Daniela P.Rastelli, Alessandra N. S. [UNESP]Peitl, OscarPranke, PatriciaZanotto, Edgar D.2018-11-26T17:39:44Z2018-11-26T17:39:44Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article233-241application/pdfhttp://dx.doi.org/10.1016/j.msec.2017.03.056Materials Science & Engineering C-materials For Biological Applications. Amsterdam: Elsevier Science Bv, v. 76, p. 233-241, 2017.0928-4931http://hdl.handle.net/11449/16300410.1016/j.msec.2017.03.056WOS:000405541600029WOS000405541600029.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science & Engineering C-materials For Biological Applications1,110info:eu-repo/semantics/openAccess2023-10-02T06:08:51Zoai:repositorio.unesp.br:11449/163004Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:49:29.162893Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
title |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
spellingShingle |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans Siqueira, Renato L. Bioactive glass Sol-gel In vitro bioactivity Cell viability Stem cells Biofilm |
title_short |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
title_full |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
title_fullStr |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
title_full_unstemmed |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
title_sort |
Bioactive gel-glasses with distinctly different compositions: Bioactivity, viability of stem cells and antibiofilm effect against Streptococcus mutans |
author |
Siqueira, Renato L. |
author_facet |
Siqueira, Renato L. Maurmann, Natasha Burguez, Daniela Pereira, Daniela P. Rastelli, Alessandra N. S. [UNESP] Peitl, Oscar Pranke, Patricia Zanotto, Edgar D. |
author_role |
author |
author2 |
Maurmann, Natasha Burguez, Daniela Pereira, Daniela P. Rastelli, Alessandra N. S. [UNESP] Peitl, Oscar Pranke, Patricia Zanotto, Edgar D. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Univ Fed Rio Grande do Sul Universidade Estadual Paulista (Unesp) Inst Pesquisa Celulas Tronco Ctr Res Technol & Educ Vitreous Mat CeRTEV |
dc.contributor.author.fl_str_mv |
Siqueira, Renato L. Maurmann, Natasha Burguez, Daniela Pereira, Daniela P. Rastelli, Alessandra N. S. [UNESP] Peitl, Oscar Pranke, Patricia Zanotto, Edgar D. |
dc.subject.por.fl_str_mv |
Bioactive glass Sol-gel In vitro bioactivity Cell viability Stem cells Biofilm |
topic |
Bioactive glass Sol-gel In vitro bioactivity Cell viability Stem cells Biofilm |
description |
In this study, an evaluation was performed to determine the in vitro bioactivity, viability of stem cells, and antibiofilm effect against Streptococcus mutans of two bioactive gel-glass 60SiO(2)-36CaO-4P(2)O(5) (BG-A) and 80SiO(2)-15CaO-5P(2)O(5) (BG-B) compositions. Both materials were bioactive and undergo the formation of hydroxycarbonate apatite (HCA) on their surfaces when immersed in simulated body fluid (SBF) after 12 h, but the BG-A composition showed a more significant formation rate. The pH variation of the samples during the test in SBF indicated that an abrupt change had occurred for the BG-A composition within the first few hours, and the pH was subsequently maintained over time, supporting its stronger antibacterial effects against S. mutans. For the in vitro viability test using mesenchymal stem cells (MSCs), the BG-B showed significantly higher cell viability compared to the BG-A composition at concentrations of 0.125, 1.25 and 12.50 mg/mL for 2 days. These results indicated that the higher solubility of the BG-A glass favors bioactivity and antibacterial effects. However, as a result of rapid degradation, the increase in the concentration of ions in the cell culture medium was not favorable for cell proliferation. Thus, by varying the composition of glasses, and consequently their dissolution rate, it is possible to favor bioactivity, antimicrobial activity or stem cell proliferation for a particular application of interest. (C) 2017 Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-11-26T17:39:44Z 2018-11-26T17:39:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2017.03.056 Materials Science & Engineering C-materials For Biological Applications. Amsterdam: Elsevier Science Bv, v. 76, p. 233-241, 2017. 0928-4931 http://hdl.handle.net/11449/163004 10.1016/j.msec.2017.03.056 WOS:000405541600029 WOS000405541600029.pdf |
url |
http://dx.doi.org/10.1016/j.msec.2017.03.056 http://hdl.handle.net/11449/163004 |
identifier_str_mv |
Materials Science & Engineering C-materials For Biological Applications. Amsterdam: Elsevier Science Bv, v. 76, p. 233-241, 2017. 0928-4931 10.1016/j.msec.2017.03.056 WOS:000405541600029 WOS000405541600029.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science & Engineering C-materials For Biological Applications 1,110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
233-241 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128279589158912 |