Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats

Detalhes bibliográficos
Autor(a) principal: Spadella, C. T. [UNESP]
Data de Publicação: 1996
Outros Autores: Mercadante, M. C.S. [UNESP], Schellini, S. A. [UNESP], Machado, J. L.M. [UNESP], Oliveira, W. K. [UNESP], Bacchi, C. E. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224018
Resumo: We studied the effects of pancreas transplantation on kidney lesions of rats with alloxan-induced diabetes. Ninety inbred male Lewis rats were randomly assigned to 3 experimental groups: group NC included 30 non-diabetic control rats, group DC included 30 alloxan-induced diabetic control rats, and group PT included 30 alloxan-induced diabetic rats that received pancreas transplants from normal donor Lewis rats. Each group was further divided into 3 subgroups of 10 rats which were sacrificed at 1, 3, and 6 months of follow-up, respectively. Clinical and laboratory parameters during these periods were documented. The kidneys of 5 rats in each subgroup were studied and 50 glomeruli and tubules from each kidney were analyzed by light microscopy by two different investigators in a double-blind study. There was progressive glomerular basement membrane thickening (GBMT), mesangial enlargement (ME), and Bowman's capsule thickening (BCT) in kidneys of rats in the 3 experimental groups during follow-up. These alterations were significantly higher in DC rats (GBMT: 1.99 ± 0.31; ME: 2.00 ± 0.33; BCT: 1.88 ± 0.27) when compared to NC (GBMT: 1.54 ± 0.30; ME: 1.56 ± 0.47; BCT: 1.36 ± 0.35) and PT rats (GBMT: 1.49 ± 0.29; ME: 1.57 ± 0.36; BCT: 1.35 ± 0.28) at 6 months (P < 0.01). The extent of GBMT, ME, and BCT observed in DC rats at 1 and 3 months was not significantly different from NC and PT rats. The amount of kidney lesions in PT rats was similar to that of NC rats and lower than those of DC rats at 6 months (P < 0.01). In addition, Armanni-Ebstein lesions of the tubules (AE) and tubular lumen protein (PRO) observed in DC rats were not present in NC or PT rats. We conclude that pancreas transplantation in alloxan-induced diabetic rats prevents the development of kidney lesions beginning at 6 months after transplantation.
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spelling Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic ratsalloxan-induced diabetesdiabetic nephropathypancreas transplantationWe studied the effects of pancreas transplantation on kidney lesions of rats with alloxan-induced diabetes. Ninety inbred male Lewis rats were randomly assigned to 3 experimental groups: group NC included 30 non-diabetic control rats, group DC included 30 alloxan-induced diabetic control rats, and group PT included 30 alloxan-induced diabetic rats that received pancreas transplants from normal donor Lewis rats. Each group was further divided into 3 subgroups of 10 rats which were sacrificed at 1, 3, and 6 months of follow-up, respectively. Clinical and laboratory parameters during these periods were documented. The kidneys of 5 rats in each subgroup were studied and 50 glomeruli and tubules from each kidney were analyzed by light microscopy by two different investigators in a double-blind study. There was progressive glomerular basement membrane thickening (GBMT), mesangial enlargement (ME), and Bowman's capsule thickening (BCT) in kidneys of rats in the 3 experimental groups during follow-up. These alterations were significantly higher in DC rats (GBMT: 1.99 ± 0.31; ME: 2.00 ± 0.33; BCT: 1.88 ± 0.27) when compared to NC (GBMT: 1.54 ± 0.30; ME: 1.56 ± 0.47; BCT: 1.36 ± 0.35) and PT rats (GBMT: 1.49 ± 0.29; ME: 1.57 ± 0.36; BCT: 1.35 ± 0.28) at 6 months (P < 0.01). The extent of GBMT, ME, and BCT observed in DC rats at 1 and 3 months was not significantly different from NC and PT rats. The amount of kidney lesions in PT rats was similar to that of NC rats and lower than those of DC rats at 6 months (P < 0.01). In addition, Armanni-Ebstein lesions of the tubules (AE) and tubular lumen protein (PRO) observed in DC rats were not present in NC or PT rats. We conclude that pancreas transplantation in alloxan-induced diabetic rats prevents the development of kidney lesions beginning at 6 months after transplantation.Departamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPDepto. Oftalmol. O. Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPDepartamento de Patologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPDepartamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPDepto. Oftalmol. O. Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPDepartamento de Patologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista, 18618-970 Botucatu, SPUniversidade Estadual Paulista (UNESP)Spadella, C. T. [UNESP]Mercadante, M. C.S. [UNESP]Schellini, S. A. [UNESP]Machado, J. L.M. [UNESP]Oliveira, W. K. [UNESP]Bacchi, C. E. [UNESP]2022-04-28T19:54:20Z2022-04-28T19:54:20Z1996-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1019-1024Brazilian Journal of Medical and Biological Research, v. 29, n. 8, p. 1019-1024, 1996.0100-879Xhttp://hdl.handle.net/11449/2240182-s2.0-0029784720Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2024-09-03T13:18:34Zoai:repositorio.unesp.br:11449/224018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
title Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
spellingShingle Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
Spadella, C. T. [UNESP]
alloxan-induced diabetes
diabetic nephropathy
pancreas transplantation
title_short Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
title_full Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
title_fullStr Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
title_full_unstemmed Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
title_sort Effect of pancreas transplantation on the prevention of nephropathy in alloxan-induced diabetic rats
author Spadella, C. T. [UNESP]
author_facet Spadella, C. T. [UNESP]
Mercadante, M. C.S. [UNESP]
Schellini, S. A. [UNESP]
Machado, J. L.M. [UNESP]
Oliveira, W. K. [UNESP]
Bacchi, C. E. [UNESP]
author_role author
author2 Mercadante, M. C.S. [UNESP]
Schellini, S. A. [UNESP]
Machado, J. L.M. [UNESP]
Oliveira, W. K. [UNESP]
Bacchi, C. E. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Spadella, C. T. [UNESP]
Mercadante, M. C.S. [UNESP]
Schellini, S. A. [UNESP]
Machado, J. L.M. [UNESP]
Oliveira, W. K. [UNESP]
Bacchi, C. E. [UNESP]
dc.subject.por.fl_str_mv alloxan-induced diabetes
diabetic nephropathy
pancreas transplantation
topic alloxan-induced diabetes
diabetic nephropathy
pancreas transplantation
description We studied the effects of pancreas transplantation on kidney lesions of rats with alloxan-induced diabetes. Ninety inbred male Lewis rats were randomly assigned to 3 experimental groups: group NC included 30 non-diabetic control rats, group DC included 30 alloxan-induced diabetic control rats, and group PT included 30 alloxan-induced diabetic rats that received pancreas transplants from normal donor Lewis rats. Each group was further divided into 3 subgroups of 10 rats which were sacrificed at 1, 3, and 6 months of follow-up, respectively. Clinical and laboratory parameters during these periods were documented. The kidneys of 5 rats in each subgroup were studied and 50 glomeruli and tubules from each kidney were analyzed by light microscopy by two different investigators in a double-blind study. There was progressive glomerular basement membrane thickening (GBMT), mesangial enlargement (ME), and Bowman's capsule thickening (BCT) in kidneys of rats in the 3 experimental groups during follow-up. These alterations were significantly higher in DC rats (GBMT: 1.99 ± 0.31; ME: 2.00 ± 0.33; BCT: 1.88 ± 0.27) when compared to NC (GBMT: 1.54 ± 0.30; ME: 1.56 ± 0.47; BCT: 1.36 ± 0.35) and PT rats (GBMT: 1.49 ± 0.29; ME: 1.57 ± 0.36; BCT: 1.35 ± 0.28) at 6 months (P < 0.01). The extent of GBMT, ME, and BCT observed in DC rats at 1 and 3 months was not significantly different from NC and PT rats. The amount of kidney lesions in PT rats was similar to that of NC rats and lower than those of DC rats at 6 months (P < 0.01). In addition, Armanni-Ebstein lesions of the tubules (AE) and tubular lumen protein (PRO) observed in DC rats were not present in NC or PT rats. We conclude that pancreas transplantation in alloxan-induced diabetic rats prevents the development of kidney lesions beginning at 6 months after transplantation.
publishDate 1996
dc.date.none.fl_str_mv 1996-08-01
2022-04-28T19:54:20Z
2022-04-28T19:54:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Journal of Medical and Biological Research, v. 29, n. 8, p. 1019-1024, 1996.
0100-879X
http://hdl.handle.net/11449/224018
2-s2.0-0029784720
identifier_str_mv Brazilian Journal of Medical and Biological Research, v. 29, n. 8, p. 1019-1024, 1996.
0100-879X
2-s2.0-0029784720
url http://hdl.handle.net/11449/224018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1019-1024
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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