Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-04130-0 http://hdl.handle.net/11449/229360 |
Resumo: | Objectives: Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods: HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results: Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion: Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder. Clinical relevance: With the limitations of an in vitro design, our study suggests that in terms of cell response, trehalose-based air-polishing powders show a reduced effect on inflammation. |
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Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitroAir-polishingCell biologyGlycinePeriodontologyTrehaloseObjectives: Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods: HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results: Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion: Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder. Clinical relevance: With the limitations of an in vitro design, our study suggests that in terms of cell response, trehalose-based air-polishing powders show a reduced effect on inflammation.Universitätsmedizin der Johannes Gutenberg-Universität MainzDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University Mainz, Augustusplatz 2Department of Periodontology University of Bern, Freiburgstrasse 7Department of Diagnosis and Surgery School of Dentistry At Araraquara São Paulo State UniversityDepartment of Diagnosis and Surgery School of Dentistry At Araraquara São Paulo State UniversityUniversidade de São Paulo (USP)University of BernUniversidade Estadual Paulista (UNESP)Weusmann, JensDeschner, JamesImber, Jean-ClaudeDamanaki, AnnaLeguizamón, Natalia D. P. [UNESP]Nogueira, Andressa V. B.2022-04-29T08:32:07Z2022-04-29T08:32:07Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1569-1578http://dx.doi.org/10.1007/s00784-021-04130-0Clinical Oral Investigations, v. 26, n. 2, p. 1569-1578, 2022.1436-37711432-6981http://hdl.handle.net/11449/22936010.1007/s00784-021-04130-02-s2.0-85112785476Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2022-04-29T08:32:07Zoai:repositorio.unesp.br:11449/229360Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:13:31.360626Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
title |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
spellingShingle |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro Weusmann, Jens Air-polishing Cell biology Glycine Periodontology Trehalose |
title_short |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
title_full |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
title_fullStr |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
title_full_unstemmed |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
title_sort |
Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro |
author |
Weusmann, Jens |
author_facet |
Weusmann, Jens Deschner, James Imber, Jean-Claude Damanaki, Anna Leguizamón, Natalia D. P. [UNESP] Nogueira, Andressa V. B. |
author_role |
author |
author2 |
Deschner, James Imber, Jean-Claude Damanaki, Anna Leguizamón, Natalia D. P. [UNESP] Nogueira, Andressa V. B. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) University of Bern Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Weusmann, Jens Deschner, James Imber, Jean-Claude Damanaki, Anna Leguizamón, Natalia D. P. [UNESP] Nogueira, Andressa V. B. |
dc.subject.por.fl_str_mv |
Air-polishing Cell biology Glycine Periodontology Trehalose |
topic |
Air-polishing Cell biology Glycine Periodontology Trehalose |
description |
Objectives: Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods: HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results: Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion: Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder. Clinical relevance: With the limitations of an in vitro design, our study suggests that in terms of cell response, trehalose-based air-polishing powders show a reduced effect on inflammation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:32:07Z 2022-04-29T08:32:07Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-04130-0 Clinical Oral Investigations, v. 26, n. 2, p. 1569-1578, 2022. 1436-3771 1432-6981 http://hdl.handle.net/11449/229360 10.1007/s00784-021-04130-0 2-s2.0-85112785476 |
url |
http://dx.doi.org/10.1007/s00784-021-04130-0 http://hdl.handle.net/11449/229360 |
identifier_str_mv |
Clinical Oral Investigations, v. 26, n. 2, p. 1569-1578, 2022. 1436-3771 1432-6981 10.1007/s00784-021-04130-0 2-s2.0-85112785476 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1569-1578 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129500392718336 |