Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes

Detalhes bibliográficos
Autor(a) principal: Barbosa, Fernando Cesar
Data de Publicação: 2020
Outros Autores: Kendrick, Emanuele, Brenelli, Livia Beatriz, Arruda, Henrique Silvano, Pastore, Glaucia Maria, Rabelo, Sarita Cândida [UNESP], Damasio, André, Franco, Telma Teixeira, Leak, David, Goldbeck, Rosana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biombioe.2020.105697
http://hdl.handle.net/11449/199214
Resumo: Enzymatic hydrolysis of lignocellulosic biomass accounts for 20–30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydrogenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCel61A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8–2.7-fold more than the commercial enzyme cocktails Cellic® Ctec2 and Celluclast® 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application.
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spelling Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymesCello-oligosaccharidesCellobiose dehydrogenaseEndoglucanasesEnzymatic hydrolysisLytic polysaccharide monooxygenaseSecond-generation bioethanolEnzymatic hydrolysis of lignocellulosic biomass accounts for 20–30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydrogenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCel61A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8–2.7-fold more than the commercial enzyme cocktails Cellic® Ctec2 and Celluclast® 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Bioprocess and Metabolic Engineering Laboratory School of Food Engineering University of Campinas (UNICAMP)Department of Biology & Biochemistry Faculty of Sciences University of BathInterdisciplinary Center of Energy Planning University of CampinasBrazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM)Bioflavors and Bioactive Compounds Laboratory Department of Food Science School of Food Engineering University of CampinasDepartment of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP)Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP)Laboratory of Biochemical Engineering Biorefining and Products of Renewable Origin (LEBBPOR) Faculty of Chemical Engineering University of Campinas (UNICAMP)Department of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP)Universidade Estadual de Campinas (UNICAMP)University of BathBrazilian Center for Research in Energy and Materials (CNPEM)Universidade Estadual Paulista (Unesp)Barbosa, Fernando CesarKendrick, EmanueleBrenelli, Livia BeatrizArruda, Henrique SilvanoPastore, Glaucia MariaRabelo, Sarita Cândida [UNESP]Damasio, AndréFranco, Telma TeixeiraLeak, DavidGoldbeck, Rosana2020-12-12T01:33:52Z2020-12-12T01:33:52Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biombioe.2020.105697Biomass and Bioenergy, v. 141.1873-29090961-9534http://hdl.handle.net/11449/19921410.1016/j.biombioe.2020.1056972-s2.0-85088999649Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomass and Bioenergyinfo:eu-repo/semantics/openAccess2022-03-14T22:34:05Zoai:repositorio.unesp.br:11449/199214Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:52:01.454327Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
title Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
spellingShingle Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
Barbosa, Fernando Cesar
Cello-oligosaccharides
Cellobiose dehydrogenase
Endoglucanases
Enzymatic hydrolysis
Lytic polysaccharide monooxygenase
Second-generation bioethanol
title_short Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
title_full Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
title_fullStr Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
title_full_unstemmed Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
title_sort Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes
author Barbosa, Fernando Cesar
author_facet Barbosa, Fernando Cesar
Kendrick, Emanuele
Brenelli, Livia Beatriz
Arruda, Henrique Silvano
Pastore, Glaucia Maria
Rabelo, Sarita Cândida [UNESP]
Damasio, André
Franco, Telma Teixeira
Leak, David
Goldbeck, Rosana
author_role author
author2 Kendrick, Emanuele
Brenelli, Livia Beatriz
Arruda, Henrique Silvano
Pastore, Glaucia Maria
Rabelo, Sarita Cândida [UNESP]
Damasio, André
Franco, Telma Teixeira
Leak, David
Goldbeck, Rosana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
University of Bath
Brazilian Center for Research in Energy and Materials (CNPEM)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Barbosa, Fernando Cesar
Kendrick, Emanuele
Brenelli, Livia Beatriz
Arruda, Henrique Silvano
Pastore, Glaucia Maria
Rabelo, Sarita Cândida [UNESP]
Damasio, André
Franco, Telma Teixeira
Leak, David
Goldbeck, Rosana
dc.subject.por.fl_str_mv Cello-oligosaccharides
Cellobiose dehydrogenase
Endoglucanases
Enzymatic hydrolysis
Lytic polysaccharide monooxygenase
Second-generation bioethanol
topic Cello-oligosaccharides
Cellobiose dehydrogenase
Endoglucanases
Enzymatic hydrolysis
Lytic polysaccharide monooxygenase
Second-generation bioethanol
description Enzymatic hydrolysis of lignocellulosic biomass accounts for 20–30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydrogenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCel61A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8–2.7-fold more than the commercial enzyme cocktails Cellic® Ctec2 and Celluclast® 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:33:52Z
2020-12-12T01:33:52Z
2020-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biombioe.2020.105697
Biomass and Bioenergy, v. 141.
1873-2909
0961-9534
http://hdl.handle.net/11449/199214
10.1016/j.biombioe.2020.105697
2-s2.0-85088999649
url http://dx.doi.org/10.1016/j.biombioe.2020.105697
http://hdl.handle.net/11449/199214
identifier_str_mv Biomass and Bioenergy, v. 141.
1873-2909
0961-9534
10.1016/j.biombioe.2020.105697
2-s2.0-85088999649
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomass and Bioenergy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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