Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/microorganisms9020329 http://hdl.handle.net/11449/207245 |
Resumo: | Xanthomonas citri subsp. citri (X. citri) is the causal agent of Asiatic Citrus Canker (ACC), a disease that affects citrus. ACC has no cure, and growers must rely on special agricultural practices to prevent bacterial spreading. Understanding X. citri basic biology is essential to foresee potential genetic targets to control ACC. Traditionally, microbial genetics use gene deletion/disruption to investigate gene function. However, essential genes are difficult to study this way. Techniques based on small-RNAs and antisense-RNAs are powerful for gene characterization, but not yet fully explored in prokaryotes. One alternative is riboswitches, which derive from bacteria, and can control transcription/translation. Riboswitches are non-coding RNAs able to modulate gene expression in the presence of specific ligands. Here we demonstrate that the riboswitch theo/metE decreases parB expression in X. citri in a platform responsive to theophylline. By monitoring cell respiration, we showed that higher concentrations of the ligand interfered with bacterial viability. Therefore, we determined the safe dose of theophylline to be used with X. citri. Finally, in downstream investigations of parB transcription modulation, we show evidence for the fact that ParB is stable, remains functional throughout the cell cycle, and is inherited by the daughter cells upon cell division. |
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Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. CitriChromosome segregationCitrus cankerParBPNPTS138 sequenceRegulation of gene expressionXanthomonas citri subsp. citri (X. citri) is the causal agent of Asiatic Citrus Canker (ACC), a disease that affects citrus. ACC has no cure, and growers must rely on special agricultural practices to prevent bacterial spreading. Understanding X. citri basic biology is essential to foresee potential genetic targets to control ACC. Traditionally, microbial genetics use gene deletion/disruption to investigate gene function. However, essential genes are difficult to study this way. Techniques based on small-RNAs and antisense-RNAs are powerful for gene characterization, but not yet fully explored in prokaryotes. One alternative is riboswitches, which derive from bacteria, and can control transcription/translation. Riboswitches are non-coding RNAs able to modulate gene expression in the presence of specific ligands. Here we demonstrate that the riboswitch theo/metE decreases parB expression in X. citri in a platform responsive to theophylline. By monitoring cell respiration, we showed that higher concentrations of the ligand interfered with bacterial viability. Therefore, we determined the safe dose of theophylline to be used with X. citri. Finally, in downstream investigations of parB transcription modulation, we show evidence for the fact that ParB is stable, remains functional throughout the cell cycle, and is inherited by the daughter cells upon cell division.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of General and Applied Biology Biosciences Institute São Paulo State University (UNESP)Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)Citriculture Center Agronomic Institute of Sao Paulo (IAC)Department of Biochemistry and Chemistry Technology Chemistry Institute São Paulo State University (UNESP)Department of Technology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Department of General and Applied Biology Biosciences Institute São Paulo State University (UNESP)Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)Department of Biochemistry and Chemistry Technology Chemistry Institute São Paulo State University (UNESP)Department of Technology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)FAPESP: 2014/50880-0FAPESP: 2015/50162-2FAPESP: 2016/18519-0CNPq: 465440/2014-2Universidade Estadual Paulista (Unesp)Agronomic Institute of Sao Paulo (IAC)Bueno, Danilo [UNESP]Pedrolli, Danielle B. [UNESP]Martins, Paula M. M.Bocchini, Daniela A. [UNESP]Moraes, Karen C. M. [UNESP]Facincani, Agda P. [UNESP]Ferro, Jesus A. [UNESP]Varani, Alessandro M. [UNESP]Pena, Michelle M. [UNESP]Ferreira, Henrique [UNESP]2021-06-25T10:51:48Z2021-06-25T10:51:48Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-15http://dx.doi.org/10.3390/microorganisms9020329Microorganisms, v. 9, n. 2, p. 1-15, 2021.2076-2607http://hdl.handle.net/11449/20724510.3390/microorganisms90203292-s2.0-85100497237Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2021-10-23T16:37:22Zoai:repositorio.unesp.br:11449/207245Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:37:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
title |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
spellingShingle |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri Bueno, Danilo [UNESP] Chromosome segregation Citrus canker ParB PNPTS138 sequence Regulation of gene expression |
title_short |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
title_full |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
title_fullStr |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
title_full_unstemmed |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
title_sort |
Riboswitch theo/mete as a transcription regulation tool for xanthomonas citri subsp. Citri |
author |
Bueno, Danilo [UNESP] |
author_facet |
Bueno, Danilo [UNESP] Pedrolli, Danielle B. [UNESP] Martins, Paula M. M. Bocchini, Daniela A. [UNESP] Moraes, Karen C. M. [UNESP] Facincani, Agda P. [UNESP] Ferro, Jesus A. [UNESP] Varani, Alessandro M. [UNESP] Pena, Michelle M. [UNESP] Ferreira, Henrique [UNESP] |
author_role |
author |
author2 |
Pedrolli, Danielle B. [UNESP] Martins, Paula M. M. Bocchini, Daniela A. [UNESP] Moraes, Karen C. M. [UNESP] Facincani, Agda P. [UNESP] Ferro, Jesus A. [UNESP] Varani, Alessandro M. [UNESP] Pena, Michelle M. [UNESP] Ferreira, Henrique [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Agronomic Institute of Sao Paulo (IAC) |
dc.contributor.author.fl_str_mv |
Bueno, Danilo [UNESP] Pedrolli, Danielle B. [UNESP] Martins, Paula M. M. Bocchini, Daniela A. [UNESP] Moraes, Karen C. M. [UNESP] Facincani, Agda P. [UNESP] Ferro, Jesus A. [UNESP] Varani, Alessandro M. [UNESP] Pena, Michelle M. [UNESP] Ferreira, Henrique [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome segregation Citrus canker ParB PNPTS138 sequence Regulation of gene expression |
topic |
Chromosome segregation Citrus canker ParB PNPTS138 sequence Regulation of gene expression |
description |
Xanthomonas citri subsp. citri (X. citri) is the causal agent of Asiatic Citrus Canker (ACC), a disease that affects citrus. ACC has no cure, and growers must rely on special agricultural practices to prevent bacterial spreading. Understanding X. citri basic biology is essential to foresee potential genetic targets to control ACC. Traditionally, microbial genetics use gene deletion/disruption to investigate gene function. However, essential genes are difficult to study this way. Techniques based on small-RNAs and antisense-RNAs are powerful for gene characterization, but not yet fully explored in prokaryotes. One alternative is riboswitches, which derive from bacteria, and can control transcription/translation. Riboswitches are non-coding RNAs able to modulate gene expression in the presence of specific ligands. Here we demonstrate that the riboswitch theo/metE decreases parB expression in X. citri in a platform responsive to theophylline. By monitoring cell respiration, we showed that higher concentrations of the ligand interfered with bacterial viability. Therefore, we determined the safe dose of theophylline to be used with X. citri. Finally, in downstream investigations of parB transcription modulation, we show evidence for the fact that ParB is stable, remains functional throughout the cell cycle, and is inherited by the daughter cells upon cell division. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:51:48Z 2021-06-25T10:51:48Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/microorganisms9020329 Microorganisms, v. 9, n. 2, p. 1-15, 2021. 2076-2607 http://hdl.handle.net/11449/207245 10.3390/microorganisms9020329 2-s2.0-85100497237 |
url |
http://dx.doi.org/10.3390/microorganisms9020329 http://hdl.handle.net/11449/207245 |
identifier_str_mv |
Microorganisms, v. 9, n. 2, p. 1-15, 2021. 2076-2607 10.3390/microorganisms9020329 2-s2.0-85100497237 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-15 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965072665083904 |