Asymmetric chromosome segregation in Xanthomonas citri ssp. citri

Detalhes bibliográficos
Autor(a) principal: Ucci, Amanda P. [UNESP]
Data de Publicação: 2014
Outros Autores: Martins, Paula M. M. [UNESP], Lau, Ivy F. [UNESP], Bacci, Maurício [UNESP], Belasque, Jose, Ferreira, Henrique [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/mbo3.145
http://hdl.handle.net/11449/116206
Resumo: This study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.
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spelling Asymmetric chromosome segregation in Xanthomonas citri ssp. citriCell division arrestchromosome segregationcitrus cankerThis study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, BrazilFundo Defesa Citricultura, BR-14807040 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, BrazilFAPESP: 10/02041-8FAPESP: 04/09173-6FAPESP: 10/05099-7CNPq: 142293/2009-1Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Fundo Defesa CitriculturaUcci, Amanda P. [UNESP]Martins, Paula M. M. [UNESP]Lau, Ivy F. [UNESP]Bacci, Maurício [UNESP]Belasque, JoseFerreira, Henrique [UNESP]2015-03-18T15:52:35Z2015-03-18T15:52:35Z2014-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29-41application/pdfhttp://dx.doi.org/10.1002/mbo3.145Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014.2045-8827http://hdl.handle.net/11449/11620610.1002/mbo3.145WOS:000342352300003WOS000342352300003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiologyopen2.6821,158info:eu-repo/semantics/openAccess2024-06-24T13:08:35Zoai:repositorio.unesp.br:11449/116206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:39:45.461354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
title Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
spellingShingle Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
Ucci, Amanda P. [UNESP]
Cell division arrest
chromosome segregation
citrus canker
title_short Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
title_full Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
title_fullStr Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
title_full_unstemmed Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
title_sort Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
author Ucci, Amanda P. [UNESP]
author_facet Ucci, Amanda P. [UNESP]
Martins, Paula M. M. [UNESP]
Lau, Ivy F. [UNESP]
Bacci, Maurício [UNESP]
Belasque, Jose
Ferreira, Henrique [UNESP]
author_role author
author2 Martins, Paula M. M. [UNESP]
Lau, Ivy F. [UNESP]
Bacci, Maurício [UNESP]
Belasque, Jose
Ferreira, Henrique [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Fundo Defesa Citricultura
dc.contributor.author.fl_str_mv Ucci, Amanda P. [UNESP]
Martins, Paula M. M. [UNESP]
Lau, Ivy F. [UNESP]
Bacci, Maurício [UNESP]
Belasque, Jose
Ferreira, Henrique [UNESP]
dc.subject.por.fl_str_mv Cell division arrest
chromosome segregation
citrus canker
topic Cell division arrest
chromosome segregation
citrus canker
description This study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-01
2015-03-18T15:52:35Z
2015-03-18T15:52:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/mbo3.145
Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014.
2045-8827
http://hdl.handle.net/11449/116206
10.1002/mbo3.145
WOS:000342352300003
WOS000342352300003.pdf
url http://dx.doi.org/10.1002/mbo3.145
http://hdl.handle.net/11449/116206
identifier_str_mv Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014.
2045-8827
10.1002/mbo3.145
WOS:000342352300003
WOS000342352300003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbiologyopen
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1,158
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 29-41
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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