Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/mbo3.145 http://hdl.handle.net/11449/116206 |
Resumo: | This study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant. |
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Repositório Institucional da UNESP |
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Asymmetric chromosome segregation in Xanthomonas citri ssp. citriCell division arrestchromosome segregationcitrus cankerThis study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, BrazilFundo Defesa Citricultura, BR-14807040 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP, BrazilFAPESP: 10/02041-8FAPESP: 04/09173-6FAPESP: 10/05099-7CNPq: 142293/2009-1Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Fundo Defesa CitriculturaUcci, Amanda P. [UNESP]Martins, Paula M. M. [UNESP]Lau, Ivy F. [UNESP]Bacci, Maurício [UNESP]Belasque, JoseFerreira, Henrique [UNESP]2015-03-18T15:52:35Z2015-03-18T15:52:35Z2014-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29-41application/pdfhttp://dx.doi.org/10.1002/mbo3.145Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014.2045-8827http://hdl.handle.net/11449/11620610.1002/mbo3.145WOS:000342352300003WOS000342352300003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiologyopen2.6821,158info:eu-repo/semantics/openAccess2024-06-24T13:08:35Zoai:repositorio.unesp.br:11449/116206Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:39:45.461354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
title |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
spellingShingle |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri Ucci, Amanda P. [UNESP] Cell division arrest chromosome segregation citrus canker |
title_short |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
title_full |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
title_fullStr |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
title_full_unstemmed |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
title_sort |
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri |
author |
Ucci, Amanda P. [UNESP] |
author_facet |
Ucci, Amanda P. [UNESP] Martins, Paula M. M. [UNESP] Lau, Ivy F. [UNESP] Bacci, Maurício [UNESP] Belasque, Jose Ferreira, Henrique [UNESP] |
author_role |
author |
author2 |
Martins, Paula M. M. [UNESP] Lau, Ivy F. [UNESP] Bacci, Maurício [UNESP] Belasque, Jose Ferreira, Henrique [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fundo Defesa Citricultura |
dc.contributor.author.fl_str_mv |
Ucci, Amanda P. [UNESP] Martins, Paula M. M. [UNESP] Lau, Ivy F. [UNESP] Bacci, Maurício [UNESP] Belasque, Jose Ferreira, Henrique [UNESP] |
dc.subject.por.fl_str_mv |
Cell division arrest chromosome segregation citrus canker |
topic |
Cell division arrest chromosome segregation citrus canker |
description |
This study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 2015-03-18T15:52:35Z 2015-03-18T15:52:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/mbo3.145 Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014. 2045-8827 http://hdl.handle.net/11449/116206 10.1002/mbo3.145 WOS:000342352300003 WOS000342352300003.pdf |
url |
http://dx.doi.org/10.1002/mbo3.145 http://hdl.handle.net/11449/116206 |
identifier_str_mv |
Microbiologyopen. Hoboken: Wiley-blackwell, v. 3, n. 1, p. 29-41, 2014. 2045-8827 10.1002/mbo3.145 WOS:000342352300003 WOS000342352300003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbiologyopen 2.682 1,158 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
29-41 application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129540378066944 |