Dimorfismo sexual relacionado às vias de reabsorção e formação de tecido ósseo de ratos e ratas castrados, após a exodontia do incisivo superior: avaliação microtomográfica e por PCR em tempo real

• Autor(a) principal: Hassumi, Jaqueline Suemi [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/204043; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-05-29/000914748.pdf
• topic: Osteoporose; Caracteres sexuais; Expressão gênica; Alvéolo dental; Microtomografia por raio-X; Reação em cadeia da polimerase; Osteoporosis

The osteoporosis is a systemic, multifactorial and progressive disease. It is typified decrease, deterioration and modification of macro and microarchitecture of bone tissue. A long time, it was considered a woman illness, however, it is being recognized as a man illness. Thus, the objective of this study was to observe how the resorption and formation pathways behave in the animals with predisposition to osteoporosis, both male and female. For this purpose, 30 male rats and 30 female rats were used and divided into groups: SHAM (♀/♂) and castrated, that they were orchiectomized ♂ (ORQ) and ovariectomized ♀ (OVX). In this way, after 30 days of the castration, the animals were submitted to extraction of right upper incisor and divided in according with the euthanasia time, that it was 14 and 42 days after the extraction surgery and analysis method of the alveolus in repair, real time Polymerase Chain Reaction (PCR) and microcomputerized tomography (micro-ct). The quantitative data were submitted to Shapiro Wilk test, Anova test and Tukey post-test with 5% of significance level. In the gene expression analysis, it was observed that the alveolar repair process after 14 days of extraction surgery, in OVX showed a significant increase of gene expression coding for alkaline phosphatase (ALP) and osteocalcin (OC). In ORQ, results showed a peak of gene expression for ALP and receptor activator of nuclear factor kappa-β ligand (RANKL). However, after 42 days of extraction surgery, OVX and ORQ showed a decrease in gene expression for osteoprotegerin and RANKL. For micro-ct analysis of alveolus repair after 42 days extraction surgery, OVX showed low percentage of bone volume and the largest separation between bone trabecular and right percentage of bone porosity. The ORQ group showed decrease bone volume, trabecular bone thickness and separation between trabecular bone. Thus, within the limits this study...