Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992009000400010 http://hdl.handle.net/11449/225737 |
Resumo: | In vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs. |
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Repositório Institucional da UNESP |
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Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazoleAmphotericin BAntifungal susceptibilityItraconazoleP. brasiliensisIn vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs.Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970Departamento de Microbiologia e Imunologia Instituto de Biociências UNESP, Botucatu, SP, 18618-970Universidade Estadual Paulista (UNESP)Takahagi-Nakaira, E. [UNESP]Sugizaki, M. F. [UNESP]Peraçoli, Maria Terezinha S. [UNESP]2022-04-28T20:59:28Z2022-04-28T20:59:28Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article718-731http://dx.doi.org/10.1590/S1678-91992009000400010Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009.1678-9199http://hdl.handle.net/11449/22573710.1590/S1678-919920090004000102-s2.0-74049083813Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T20:59:28Zoai:repositorio.unesp.br:11449/225737Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:52:02.906533Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
title |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
spellingShingle |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole Takahagi-Nakaira, E. [UNESP] Amphotericin B Antifungal susceptibility Itraconazole P. brasiliensis |
title_short |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
title_full |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
title_fullStr |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
title_full_unstemmed |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
title_sort |
Microdilution procedure for antifungal susceptibility testing of Paracoccidioides brasiliensis to amphotericin b and itraconazole |
author |
Takahagi-Nakaira, E. [UNESP] |
author_facet |
Takahagi-Nakaira, E. [UNESP] Sugizaki, M. F. [UNESP] Peraçoli, Maria Terezinha S. [UNESP] |
author_role |
author |
author2 |
Sugizaki, M. F. [UNESP] Peraçoli, Maria Terezinha S. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Takahagi-Nakaira, E. [UNESP] Sugizaki, M. F. [UNESP] Peraçoli, Maria Terezinha S. [UNESP] |
dc.subject.por.fl_str_mv |
Amphotericin B Antifungal susceptibility Itraconazole P. brasiliensis |
topic |
Amphotericin B Antifungal susceptibility Itraconazole P. brasiliensis |
description |
In vitro tests employing microdilution to evaluate fungal susceptibility to antifungal drugs are already standardized for fermentative yeasts. However, studies on the susceptibility of dimorphic fungi such as Paracoccidioides brasiliensis employing this method are scarce. The present work introduced some modifications into antifungal susceptibility testing from the European Committee on Antimicrobial Susceptibility Testing (EUCAST), concerning broth medium and reading time, to determine minimal inhibitory concentration (MIC) of amphotericin B and itraconazole against Paracoccidioides brasiliensis. Yeast-like cells of P. brasiliensis (Pb18 strain) were tested for susceptibility to amphotericin B and itraconazole in RPMI 1640 medium, supplemented with 2% glucose and nitrogen source and incubated at 35°C. The MIC of amphotericin B and itraconazole against Pb18 were respectively 0.25 μg/mL and 0.002 μg/mL. The results of minimal fungicidal concentration (MFC) showed that amphotericin B at 0.25 μg/mL or higher concentrations displayed fungicidal activity against Pb18 while itraconazole at least 0.002 μg/mL has a fungistatic effect on P. brasiliensis. In conclusion, our results showed that the method employed in the present study is reproducible and reliable for testing the susceptibility of P. brasiliensis to antifungal drugs. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:59:28Z 2022-04-28T20:59:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992009000400010 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009. 1678-9199 http://hdl.handle.net/11449/225737 10.1590/S1678-91992009000400010 2-s2.0-74049083813 |
url |
http://dx.doi.org/10.1590/S1678-91992009000400010 http://hdl.handle.net/11449/225737 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 4, p. 718-731, 2009. 1678-9199 10.1590/S1678-91992009000400010 2-s2.0-74049083813 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
718-731 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128713386098688 |