Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biopha.2021.111984 http://hdl.handle.net/11449/233354 |
Resumo: | HIV infection and the prolonged use of antiretroviral therapy (ART) contribute to persistent inflammation and immune deregulation in people living with HIV/AIDS (PLWHA). Propolis is a bee product with plenty of biological properties, including immunomodulatory and anti-inflammatory action. This work aimed to evaluate possible changes in the immune/inflammatory response in PLWHA under ART after propolis intake. Asymptomatic PLWHA were double-blindly randomized into parallel groups receiving propolis (500 mg/day, n = 20) for 3 months or placebo (n = 20). Plasma cytokines (TNF-α, IL-2, IL-4, IL-6, IL-10 and IL17) were evaluated by cytometric bead array; cytokine production by PBMC (IFN-γ, IL-5, IL-17, IL-10, IL-1β, IL-18, and IL-33) was assessed by ELISA; gene expression (T-bet, GATA-3, RORγt and Foxp3) was determined by RT-qPCR, and cell proliferation was analysed by flow cytometry using CFSE staining. The average of gender, age, CD4+/CD8+ T cell count, time of diagnosis and treatment were similar in both groups. No differences were observed in cytokine levels nor in inflammasome activation. However, Pearson's correlation showed that IL-10 was directly correlated to CD4+ T cell count and inversely to IFN-γ after treatment with propolis. Foxp3 expression and lymphocyte proliferation increased in the propolis group. Data suggested that daily propolis consumption may improve the immune response and decrease the inflammatory status in asymptomatic PLWHA under ART. |
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Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled studyAntiretroviral therapyHIV/AIDSImmunomodulationInflammationPropolisTreg cellsHIV infection and the prolonged use of antiretroviral therapy (ART) contribute to persistent inflammation and immune deregulation in people living with HIV/AIDS (PLWHA). Propolis is a bee product with plenty of biological properties, including immunomodulatory and anti-inflammatory action. This work aimed to evaluate possible changes in the immune/inflammatory response in PLWHA under ART after propolis intake. Asymptomatic PLWHA were double-blindly randomized into parallel groups receiving propolis (500 mg/day, n = 20) for 3 months or placebo (n = 20). Plasma cytokines (TNF-α, IL-2, IL-4, IL-6, IL-10 and IL17) were evaluated by cytometric bead array; cytokine production by PBMC (IFN-γ, IL-5, IL-17, IL-10, IL-1β, IL-18, and IL-33) was assessed by ELISA; gene expression (T-bet, GATA-3, RORγt and Foxp3) was determined by RT-qPCR, and cell proliferation was analysed by flow cytometry using CFSE staining. The average of gender, age, CD4+/CD8+ T cell count, time of diagnosis and treatment were similar in both groups. No differences were observed in cytokine levels nor in inflammasome activation. However, Pearson's correlation showed that IL-10 was directly correlated to CD4+ T cell count and inversely to IFN-γ after treatment with propolis. Foxp3 expression and lymphocyte proliferation increased in the propolis group. Data suggested that daily propolis consumption may improve the immune response and decrease the inflammatory status in asymptomatic PLWHA under ART.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuSão Paulo State University (UNESP) Botucatu Medical School, Campus BotucatuResearch Development & Innovation Laboratory Apis Flora Indl. Coml. Ltda.São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuSão Paulo State University (UNESP) Botucatu Medical School, Campus BotucatuCNPq: 140245/2017-0CNPq: 302230/2017-3Universidade Estadual Paulista (UNESP)Apis Flora Indl. Coml. Ltda.Conte, Fernanda Lopes [UNESP]Tasca, Karen Ingrid [UNESP]Santiago, Karina Basso [UNESP]de Oliveira Cardoso, Eliza [UNESP]Romagnoli, Graziela Gorete [UNESP]de Assis Golim, Marjorie [UNESP]Braz, Aline Márcia Marques [UNESP]Berretta, Andresa Aparecidado Rosário de Souza, Lenice [UNESP]Sforcin, José Maurício [UNESP]2022-05-01T07:58:52Z2022-05-01T07:58:52Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biopha.2021.111984Biomedicine and Pharmacotherapy, v. 142.1950-60070753-3322http://hdl.handle.net/11449/23335410.1016/j.biopha.2021.1119842-s2.0-85111936725Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine and Pharmacotherapyinfo:eu-repo/semantics/openAccess2022-05-01T07:58:52Zoai:repositorio.unesp.br:11449/233354Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:36:57.322937Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
title |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
spellingShingle |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study Conte, Fernanda Lopes [UNESP] Antiretroviral therapy HIV/AIDS Immunomodulation Inflammation Propolis Treg cells |
title_short |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
title_full |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
title_fullStr |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
title_full_unstemmed |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
title_sort |
Propolis increases Foxp3 expression and lymphocyte proliferation in HIV-infected people: A randomized, double blind, parallel-group and placebo-controlled study |
author |
Conte, Fernanda Lopes [UNESP] |
author_facet |
Conte, Fernanda Lopes [UNESP] Tasca, Karen Ingrid [UNESP] Santiago, Karina Basso [UNESP] de Oliveira Cardoso, Eliza [UNESP] Romagnoli, Graziela Gorete [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Márcia Marques [UNESP] Berretta, Andresa Aparecida do Rosário de Souza, Lenice [UNESP] Sforcin, José Maurício [UNESP] |
author_role |
author |
author2 |
Tasca, Karen Ingrid [UNESP] Santiago, Karina Basso [UNESP] de Oliveira Cardoso, Eliza [UNESP] Romagnoli, Graziela Gorete [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Márcia Marques [UNESP] Berretta, Andresa Aparecida do Rosário de Souza, Lenice [UNESP] Sforcin, José Maurício [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Apis Flora Indl. Coml. Ltda. |
dc.contributor.author.fl_str_mv |
Conte, Fernanda Lopes [UNESP] Tasca, Karen Ingrid [UNESP] Santiago, Karina Basso [UNESP] de Oliveira Cardoso, Eliza [UNESP] Romagnoli, Graziela Gorete [UNESP] de Assis Golim, Marjorie [UNESP] Braz, Aline Márcia Marques [UNESP] Berretta, Andresa Aparecida do Rosário de Souza, Lenice [UNESP] Sforcin, José Maurício [UNESP] |
dc.subject.por.fl_str_mv |
Antiretroviral therapy HIV/AIDS Immunomodulation Inflammation Propolis Treg cells |
topic |
Antiretroviral therapy HIV/AIDS Immunomodulation Inflammation Propolis Treg cells |
description |
HIV infection and the prolonged use of antiretroviral therapy (ART) contribute to persistent inflammation and immune deregulation in people living with HIV/AIDS (PLWHA). Propolis is a bee product with plenty of biological properties, including immunomodulatory and anti-inflammatory action. This work aimed to evaluate possible changes in the immune/inflammatory response in PLWHA under ART after propolis intake. Asymptomatic PLWHA were double-blindly randomized into parallel groups receiving propolis (500 mg/day, n = 20) for 3 months or placebo (n = 20). Plasma cytokines (TNF-α, IL-2, IL-4, IL-6, IL-10 and IL17) were evaluated by cytometric bead array; cytokine production by PBMC (IFN-γ, IL-5, IL-17, IL-10, IL-1β, IL-18, and IL-33) was assessed by ELISA; gene expression (T-bet, GATA-3, RORγt and Foxp3) was determined by RT-qPCR, and cell proliferation was analysed by flow cytometry using CFSE staining. The average of gender, age, CD4+/CD8+ T cell count, time of diagnosis and treatment were similar in both groups. No differences were observed in cytokine levels nor in inflammasome activation. However, Pearson's correlation showed that IL-10 was directly correlated to CD4+ T cell count and inversely to IFN-γ after treatment with propolis. Foxp3 expression and lymphocyte proliferation increased in the propolis group. Data suggested that daily propolis consumption may improve the immune response and decrease the inflammatory status in asymptomatic PLWHA under ART. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-05-01T07:58:52Z 2022-05-01T07:58:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biopha.2021.111984 Biomedicine and Pharmacotherapy, v. 142. 1950-6007 0753-3322 http://hdl.handle.net/11449/233354 10.1016/j.biopha.2021.111984 2-s2.0-85111936725 |
url |
http://dx.doi.org/10.1016/j.biopha.2021.111984 http://hdl.handle.net/11449/233354 |
identifier_str_mv |
Biomedicine and Pharmacotherapy, v. 142. 1950-6007 0753-3322 10.1016/j.biopha.2021.111984 2-s2.0-85111936725 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomedicine and Pharmacotherapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129442731524096 |