Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/00222933.2020.1748243 http://hdl.handle.net/11449/198811 |
Resumo: | The subgenus Mantidactylus is a group of frogs endemic to Madagascar, including the largest anuran species on the island. Although these frogs are common and widely distributed, their taxonomy remains unclear. Two species are currently recognised, M. grandidieri and M. guttulatus, with another available name, Rana pigra, considered to be a synonym of M. grandidieri. However, molecular studies have suggested the presence of several cryptic species within the group. Additionally, due to the lack of prominent morphological features, allocating the available names to evolutionary lineages has proven challenging. In the present study, we take a first step towards solving these problems by using fragments of the 16S mitochondrial gene and RAG1 nuclear gene from all over the range of the subgenus to describe its genetic diversity. We also use a newly designed target enrichment laboratory protocol to sequence three mitochondrial fragments from five name-holding museum specimens (as old as 120 years) in order to determine to which lineages the existing names should be applied. The study of the 16S mitochondrial gene revealed 7 geographically separated lineages, distinct enough to be considered candidate species. Out of the five museum specimens analysed, four successfully yielded DNA sequences and could be attributed to one of the aforementioned lineages. Therefore, the name Mantidactylus grandidieri should be applied to the populations from North-Eastern Madagascar, while M. guttulatus refers to populations from inland localities along the Eastern coast of the island. On the other hand, the holotype of Rana pigra did not yield enough genetic material to allow definitive identification. While our data were not sufficient to assess the status of the four lineages distributed along the Eastern coast, the populations from North-Western Madagascar were highly distinct on both the mitochondrial and nuclear markers. We thus describe them as a new species, M. radaka sp. nov. http://www.zoobank.org/urn:lsid:zoobank.org:pub:1EDDAF0D-FE37-490A-B09E-E136A0C5CB35. |
id |
UNSP_70e18afd390e082660fb175fe6d9db08 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/198811 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus)AnuraMadagascarMantidactylus grandidieriMantidactylus guttulatusMantidactylus radaka sp. novTarget enrichmentThe subgenus Mantidactylus is a group of frogs endemic to Madagascar, including the largest anuran species on the island. Although these frogs are common and widely distributed, their taxonomy remains unclear. Two species are currently recognised, M. grandidieri and M. guttulatus, with another available name, Rana pigra, considered to be a synonym of M. grandidieri. However, molecular studies have suggested the presence of several cryptic species within the group. Additionally, due to the lack of prominent morphological features, allocating the available names to evolutionary lineages has proven challenging. In the present study, we take a first step towards solving these problems by using fragments of the 16S mitochondrial gene and RAG1 nuclear gene from all over the range of the subgenus to describe its genetic diversity. We also use a newly designed target enrichment laboratory protocol to sequence three mitochondrial fragments from five name-holding museum specimens (as old as 120 years) in order to determine to which lineages the existing names should be applied. The study of the 16S mitochondrial gene revealed 7 geographically separated lineages, distinct enough to be considered candidate species. Out of the five museum specimens analysed, four successfully yielded DNA sequences and could be attributed to one of the aforementioned lineages. Therefore, the name Mantidactylus grandidieri should be applied to the populations from North-Eastern Madagascar, while M. guttulatus refers to populations from inland localities along the Eastern coast of the island. On the other hand, the holotype of Rana pigra did not yield enough genetic material to allow definitive identification. While our data were not sufficient to assess the status of the four lineages distributed along the Eastern coast, the populations from North-Western Madagascar were highly distinct on both the mitochondrial and nuclear markers. We thus describe them as a new species, M. radaka sp. nov. http://www.zoobank.org/urn:lsid:zoobank.org:pub:1EDDAF0D-FE37-490A-B09E-E136A0C5CB35.Zoological Institute Braunschweig University of TechnologySektion Herpetologie Zoologische Staatssammlung München (ZSM-SNSB)Institut de Systématique Biodiversité (ISYEB) Sorbonne UniversitéPrograma de Pós-Graduação em Biologia Animal Departamento de Biologia Animal Laboratório de Herpetologia Universidade Federal Rural do Rio de JaneiroFaculty of Mathematics and Natural Sciences Institute for Biochemistry and Biology University of PotsdamDepartment of Natural History University Museum of BergenDepto de Zoologia Instituto de Biologia Universidade Estadual Paulista - UNESPDepartment of Life Sciences The Natural History Museum, Cromwell RoadMuseo Regionale di Scienze NaturaliResearch Centre in Biodiversity Genetics and Evolution Universidade do PortoBiodiversity Institute and Department of Ecology and Evolutionary Biology University of KansasMention Zoologie et Biodiversité Animale Faculté des Sciences Université d’AntananarivoDepto de Zoologia Instituto de Biologia Universidade Estadual Paulista - UNESPBraunschweig University of TechnologyZoologische Staatssammlung München (ZSM-SNSB)Sorbonne UniversitéUniversidade Federal Rural do Rio de JaneiroUniversity of PotsdamUniversity Museum of BergenUniversidade Estadual Paulista (Unesp)The Natural History MuseumMuseo Regionale di Scienze NaturaliUniversidade do PortoUniversity of KansasUniversité d’AntananarivoRancilhac, LoïsBruy, TeddyScherz, Mark D.Pereira, Elvis AlmeidaPreick, MichaelaStraube, NicolasLyra, Mariana L. [UNESP]Ohler, AnnemarieStreicher, Jeffrey W.Andreone, FrancoCrottini, AngelicaHutter, Carl R.Randrianantoandro, J. ChristianRakotoarison, AndolalaoGlaw, FrankHofreiter, MichaelVences, Miguel2020-12-12T01:22:39Z2020-12-12T01:22:39Z2020-01-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article87-118http://dx.doi.org/10.1080/00222933.2020.1748243Journal of Natural History, v. 54, n. 1-4, p. 87-118, 2020.1464-52620022-2933http://hdl.handle.net/11449/19881110.1080/00222933.2020.17482432-s2.0-85084311964Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Natural Historyinfo:eu-repo/semantics/openAccess2021-10-22T20:28:57Zoai:repositorio.unesp.br:11449/198811Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:43.138553Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
title |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
spellingShingle |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) Rancilhac, Loïs Anura Madagascar Mantidactylus grandidieri Mantidactylus guttulatus Mantidactylus radaka sp. nov Target enrichment |
title_short |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
title_full |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
title_fullStr |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
title_full_unstemmed |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
title_sort |
Target-enriched DNA sequencing from historical type material enables a partial revision of the Madagascar giant stream frogs (genus Mantidactylus) |
author |
Rancilhac, Loïs |
author_facet |
Rancilhac, Loïs Bruy, Teddy Scherz, Mark D. Pereira, Elvis Almeida Preick, Michaela Straube, Nicolas Lyra, Mariana L. [UNESP] Ohler, Annemarie Streicher, Jeffrey W. Andreone, Franco Crottini, Angelica Hutter, Carl R. Randrianantoandro, J. Christian Rakotoarison, Andolalao Glaw, Frank Hofreiter, Michael Vences, Miguel |
author_role |
author |
author2 |
Bruy, Teddy Scherz, Mark D. Pereira, Elvis Almeida Preick, Michaela Straube, Nicolas Lyra, Mariana L. [UNESP] Ohler, Annemarie Streicher, Jeffrey W. Andreone, Franco Crottini, Angelica Hutter, Carl R. Randrianantoandro, J. Christian Rakotoarison, Andolalao Glaw, Frank Hofreiter, Michael Vences, Miguel |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Braunschweig University of Technology Zoologische Staatssammlung München (ZSM-SNSB) Sorbonne Université Universidade Federal Rural do Rio de Janeiro University of Potsdam University Museum of Bergen Universidade Estadual Paulista (Unesp) The Natural History Museum Museo Regionale di Scienze Naturali Universidade do Porto University of Kansas Université d’Antananarivo |
dc.contributor.author.fl_str_mv |
Rancilhac, Loïs Bruy, Teddy Scherz, Mark D. Pereira, Elvis Almeida Preick, Michaela Straube, Nicolas Lyra, Mariana L. [UNESP] Ohler, Annemarie Streicher, Jeffrey W. Andreone, Franco Crottini, Angelica Hutter, Carl R. Randrianantoandro, J. Christian Rakotoarison, Andolalao Glaw, Frank Hofreiter, Michael Vences, Miguel |
dc.subject.por.fl_str_mv |
Anura Madagascar Mantidactylus grandidieri Mantidactylus guttulatus Mantidactylus radaka sp. nov Target enrichment |
topic |
Anura Madagascar Mantidactylus grandidieri Mantidactylus guttulatus Mantidactylus radaka sp. nov Target enrichment |
description |
The subgenus Mantidactylus is a group of frogs endemic to Madagascar, including the largest anuran species on the island. Although these frogs are common and widely distributed, their taxonomy remains unclear. Two species are currently recognised, M. grandidieri and M. guttulatus, with another available name, Rana pigra, considered to be a synonym of M. grandidieri. However, molecular studies have suggested the presence of several cryptic species within the group. Additionally, due to the lack of prominent morphological features, allocating the available names to evolutionary lineages has proven challenging. In the present study, we take a first step towards solving these problems by using fragments of the 16S mitochondrial gene and RAG1 nuclear gene from all over the range of the subgenus to describe its genetic diversity. We also use a newly designed target enrichment laboratory protocol to sequence three mitochondrial fragments from five name-holding museum specimens (as old as 120 years) in order to determine to which lineages the existing names should be applied. The study of the 16S mitochondrial gene revealed 7 geographically separated lineages, distinct enough to be considered candidate species. Out of the five museum specimens analysed, four successfully yielded DNA sequences and could be attributed to one of the aforementioned lineages. Therefore, the name Mantidactylus grandidieri should be applied to the populations from North-Eastern Madagascar, while M. guttulatus refers to populations from inland localities along the Eastern coast of the island. On the other hand, the holotype of Rana pigra did not yield enough genetic material to allow definitive identification. While our data were not sufficient to assess the status of the four lineages distributed along the Eastern coast, the populations from North-Western Madagascar were highly distinct on both the mitochondrial and nuclear markers. We thus describe them as a new species, M. radaka sp. nov. http://www.zoobank.org/urn:lsid:zoobank.org:pub:1EDDAF0D-FE37-490A-B09E-E136A0C5CB35. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:22:39Z 2020-12-12T01:22:39Z 2020-01-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/00222933.2020.1748243 Journal of Natural History, v. 54, n. 1-4, p. 87-118, 2020. 1464-5262 0022-2933 http://hdl.handle.net/11449/198811 10.1080/00222933.2020.1748243 2-s2.0-85084311964 |
url |
http://dx.doi.org/10.1080/00222933.2020.1748243 http://hdl.handle.net/11449/198811 |
identifier_str_mv |
Journal of Natural History, v. 54, n. 1-4, p. 87-118, 2020. 1464-5262 0022-2933 10.1080/00222933.2020.1748243 2-s2.0-85084311964 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Natural History |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
87-118 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129390130757632 |