Avaliação in vitro da adesão microbiana em diferentes rugosidades de superfície de resina acrílica específica para prótese ocular

• Autor(a) principal: Sousa, Cecília Alves de [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149673; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-31/000867661.pdf
• topic: Aspereza de superfície; Resinas acrílicas; Olhos artificiais; Surface roughness

The aim of this study was to analyze the acrylic resin surface roughness for ocular prosthesis subjected to polishing with different granulation sandpapers and evaluate the effect of different polishing levels in adhesion and biofilm formation of different microorganisms (S. epidermidis, S. aureus, E. faecalis and C. albicans), on the surfaces of the acrylic resin for ocular prostheses. For this, a total of 432 samples of acrylic resin was divided into 6 groups according to the polishing (Group 1: granulating 1200 + silica solution, Group 2: Granulation 1200, Group 3: Granulation 800, Group 4: granulation 400, Group 5: granulation 120 and Group 6: unpolished). The surface roughness was measured by a profilometer and Atomic Force Microscopy. The microbial growth on samples surfaces were analyzed after 4, 24 and 48 hours of incubation. Data were submitted to statistical analysis. The results showed that the groups with higher mean values of roughness were 120 and 400. To S. epidermidis, all groups differed from the control after 4 and 48 hours. The 1200S group differed from the negative control at all periods and presneted the lowest values of bacterial growth after 24 and 48 h of growth. For S. aureus, only the 1200S group showed the lowest values of bacterial growth, with a significant difference from the negative control. For E. faecalis, all groups differed from negative control but not with each other, except 400. C. albicans did not adhere to the surfaces of acrylic resin. It is concluded that the different surface roughness did not statistically interfere with adhesion and microbial growth on the samples surfaces, except the 1200S group for the bacteria species