Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00709-018-01336-0 http://hdl.handle.net/11449/185639 |
Resumo: | Perfect flowers of Maytenus obtusifolia have partial sterility of pollen grains, resulting in collapsed and developed free microspores. However, the cellular events resulting in partial male sterility have not been determined. In pistillate flowers of this species, male sterility is related to the premature programmed cell death (PCD) in tapetum and sporogenic cells. The process occurs through autophagy via macroautophagy and massive autophagy and is associated with sporophytic cytoplasmic male sterility (CMS). Here, we characterised the development of pollen grains and investigated the cellular events that result in tapetum cells and free microspores PCD in perfect flowers, using light and transmission electron microscopy combined with the TUNEL (Terminal deoxynucleotidyl transferase mediated dUDP end-Labeling) assay and the ZIO (Zinc iodide-osmium tetroxide) method. Pollen grain development in perfect flowers was divided into eight developmental stages based on the characteristics of the pollen grains. Tapetum cells undergo PCD at the free microspore stage, through a macroautophagic process, by formation of autophagosomes and by autophagosomes giving rise to lytic vacuoles at maturity. In the final stage of PCD, massive autophagy occurs by rupture of the tonoplast. The development of viable and inviable microspores diverges at the vacuolated microspore stage, when PCD occurs in some free microspores, causing interruption of pollen development through necrosis. These events result in the observed partial male sterility. Viable microspores undergo mitosis and develop into tricellular pollen grains. Male sterility in hermaphrodite individuals is here interpreted as gametophytic CMS. |
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Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae)AnatomyAutophagyGametophytic CMSImmunocytochemistryPCDUltrastructurePerfect flowers of Maytenus obtusifolia have partial sterility of pollen grains, resulting in collapsed and developed free microspores. However, the cellular events resulting in partial male sterility have not been determined. In pistillate flowers of this species, male sterility is related to the premature programmed cell death (PCD) in tapetum and sporogenic cells. The process occurs through autophagy via macroautophagy and massive autophagy and is associated with sporophytic cytoplasmic male sterility (CMS). Here, we characterised the development of pollen grains and investigated the cellular events that result in tapetum cells and free microspores PCD in perfect flowers, using light and transmission electron microscopy combined with the TUNEL (Terminal deoxynucleotidyl transferase mediated dUDP end-Labeling) assay and the ZIO (Zinc iodide-osmium tetroxide) method. Pollen grain development in perfect flowers was divided into eight developmental stages based on the characteristics of the pollen grains. Tapetum cells undergo PCD at the free microspore stage, through a macroautophagic process, by formation of autophagosomes and by autophagosomes giving rise to lytic vacuoles at maturity. In the final stage of PCD, massive autophagy occurs by rupture of the tonoplast. The development of viable and inviable microspores diverges at the vacuolated microspore stage, when PCD occurs in some free microspores, causing interruption of pollen development through necrosis. These events result in the observed partial male sterility. Viable microspores undergo mitosis and develop into tricellular pollen grains. Male sterility in hermaphrodite individuals is here interpreted as gametophytic CMS.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Rio de Janeiro, Dept Bot, Museu Nacl, BR-22940040 Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Dept Bot, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Bot, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilFAPERJ: E-26/111.207/2014CNPq: 447624/2014-8CNPq: 304396/2015-0SpringerUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (Unesp)Nader Haddad, Isabella VerissimoSa-Haiad, Barbara deRibeiro de Santiago-Fernandes, Lygia DoloresMachado, Silvia Rodrigues [UNESP]2019-10-04T12:37:13Z2019-10-04T12:37:13Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article745-761http://dx.doi.org/10.1007/s00709-018-01336-0Protoplasma. Wien: Springer Wien, v. 256, n. 3, p. 745-761, 2019.0033-183Xhttp://hdl.handle.net/11449/18563910.1007/s00709-018-01336-0WOS:000465637300016Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProtoplasmainfo:eu-repo/semantics/openAccess2021-10-22T21:09:57Zoai:repositorio.unesp.br:11449/185639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:18:49.426921Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
title |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
spellingShingle |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) Nader Haddad, Isabella Verissimo Anatomy Autophagy Gametophytic CMS Immunocytochemistry PCD Ultrastructure |
title_short |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
title_full |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
title_fullStr |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
title_full_unstemmed |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
title_sort |
Pollen grain development and male sterility in the perfect flowers of Maytenus obtusifolia Mart. (Celastraceae) |
author |
Nader Haddad, Isabella Verissimo |
author_facet |
Nader Haddad, Isabella Verissimo Sa-Haiad, Barbara de Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
author_role |
author |
author2 |
Sa-Haiad, Barbara de Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nader Haddad, Isabella Verissimo Sa-Haiad, Barbara de Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
dc.subject.por.fl_str_mv |
Anatomy Autophagy Gametophytic CMS Immunocytochemistry PCD Ultrastructure |
topic |
Anatomy Autophagy Gametophytic CMS Immunocytochemistry PCD Ultrastructure |
description |
Perfect flowers of Maytenus obtusifolia have partial sterility of pollen grains, resulting in collapsed and developed free microspores. However, the cellular events resulting in partial male sterility have not been determined. In pistillate flowers of this species, male sterility is related to the premature programmed cell death (PCD) in tapetum and sporogenic cells. The process occurs through autophagy via macroautophagy and massive autophagy and is associated with sporophytic cytoplasmic male sterility (CMS). Here, we characterised the development of pollen grains and investigated the cellular events that result in tapetum cells and free microspores PCD in perfect flowers, using light and transmission electron microscopy combined with the TUNEL (Terminal deoxynucleotidyl transferase mediated dUDP end-Labeling) assay and the ZIO (Zinc iodide-osmium tetroxide) method. Pollen grain development in perfect flowers was divided into eight developmental stages based on the characteristics of the pollen grains. Tapetum cells undergo PCD at the free microspore stage, through a macroautophagic process, by formation of autophagosomes and by autophagosomes giving rise to lytic vacuoles at maturity. In the final stage of PCD, massive autophagy occurs by rupture of the tonoplast. The development of viable and inviable microspores diverges at the vacuolated microspore stage, when PCD occurs in some free microspores, causing interruption of pollen development through necrosis. These events result in the observed partial male sterility. Viable microspores undergo mitosis and develop into tricellular pollen grains. Male sterility in hermaphrodite individuals is here interpreted as gametophytic CMS. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:37:13Z 2019-10-04T12:37:13Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00709-018-01336-0 Protoplasma. Wien: Springer Wien, v. 256, n. 3, p. 745-761, 2019. 0033-183X http://hdl.handle.net/11449/185639 10.1007/s00709-018-01336-0 WOS:000465637300016 |
url |
http://dx.doi.org/10.1007/s00709-018-01336-0 http://hdl.handle.net/11449/185639 |
identifier_str_mv |
Protoplasma. Wien: Springer Wien, v. 256, n. 3, p. 745-761, 2019. 0033-183X 10.1007/s00709-018-01336-0 WOS:000465637300016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Protoplasma |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
745-761 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128345824559104 |