Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242008000200007 http://hdl.handle.net/11449/70518 |
Resumo: | This in situ study investigated, using scanning electron microscopy, the effect of stimulated saliva on the enamel surface of bovine and human substrates submitted to erosion followed by brushing abrasion immediately or after one hour. During 2 experimental 7-day crossover phases, 9 previously selected volunteers wore intraoral palatal devices, with 12 enamel specimens (6 human and 6 bovine). In the first phase, the volunteers immersed the device for 5 minutes in 150 ml of a cola drink, 4 times a day (8h00, 12h00, 16h00 and 20h00). Immediately after the immersions, no treatment was performed in 4 specimens (ERO), 4 other specimens were immediately brushed (0 min) using a fluoride dentifrice and the device was replaced into the mouth. After 60 min, the other 4 specimens were brushed. In the second phase, the procedures were repeated but, after the immersions, the volunteers stimulated the salivary flow rate by chewing a sugar-free gum for 30 min. Enamel superficial alterations of all specimens were then evaluated using a scanning electron microscope. Enamel prism core dissolution was seen on the surfaces submitted to erosion, while on those submitted to erosion and to abrasion (both at 0 and 60 min) a more homogeneous enamel surface was observed, probably due to the removal of the altered superficial prism layer. For all the other variables - enamel substrate and salivary stimulation the microscopic pattern of the enamel specimens was similar. |
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Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamelDental enamelScanning electron microscopyTooth abrasionTooth erosionadultanimalbeveragecarbonated beveragecattlechemistrychewing gumdental caredental cariesdrug effectenamelfemalefoodhumanmalepathologypHphysiologysalivascanning electron microscopysurface propertytooth brushingtooth diseaseultrastructureAdultAnimalsBeveragesCarbonated BeveragesCattleChewing GumDental CariesDental EnamelDental Enamel SolubilityFemaleFoodHumansHydrogen-Ion ConcentrationMaleMicroscopy, Electron, ScanningSalivaSurface PropertiesTooth AbrasionTooth ErosionTooth RemineralizationToothbrushingYoung AdultThis in situ study investigated, using scanning electron microscopy, the effect of stimulated saliva on the enamel surface of bovine and human substrates submitted to erosion followed by brushing abrasion immediately or after one hour. During 2 experimental 7-day crossover phases, 9 previously selected volunteers wore intraoral palatal devices, with 12 enamel specimens (6 human and 6 bovine). In the first phase, the volunteers immersed the device for 5 minutes in 150 ml of a cola drink, 4 times a day (8h00, 12h00, 16h00 and 20h00). Immediately after the immersions, no treatment was performed in 4 specimens (ERO), 4 other specimens were immediately brushed (0 min) using a fluoride dentifrice and the device was replaced into the mouth. After 60 min, the other 4 specimens were brushed. In the second phase, the procedures were repeated but, after the immersions, the volunteers stimulated the salivary flow rate by chewing a sugar-free gum for 30 min. Enamel superficial alterations of all specimens were then evaluated using a scanning electron microscope. Enamel prism core dissolution was seen on the surfaces submitted to erosion, while on those submitted to erosion and to abrasion (both at 0 and 60 min) a more homogeneous enamel surface was observed, probably due to the removal of the altered superficial prism layer. For all the other variables - enamel substrate and salivary stimulation the microscopic pattern of the enamel specimens was similar.Department of Biological Sciences School of Dentistry of Bauru University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP 17012-901Department of Pediatric Dentistry Federal University of Alfenas, AlfenasDepartment of Pediatric Dentistry School of Dentistry of Bauru University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP 17012-901Department of Pediatric Dentistry School of Dentistry of Araçatuba São Paulo State University, São PauloDepartment of Pediatric Dentistry School of Dentistry of Araçatuba São Paulo State University, São PauloUniversidade de São Paulo (USP)Federal University of AlfenasUniversidade Estadual Paulista (Unesp)Rios, DanielaHonório, Heitor MarquesMagalhães, Ana Carolinada Silva, Salete Moura Bonifácio [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Machado, Maria Aparecida de Andrade MoreiraBuzalaf, Marília Afonso Rabelo2014-05-27T11:23:38Z2014-05-27T11:23:38Z2008-08-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article132-138application/pdfhttp://dx.doi.org/10.1590/S1806-83242008000200007Brazilian Oral Research, v. 22, n. 2, p. 132-138, 2008.1806-83241807-3107http://hdl.handle.net/11449/7051810.1590/S1806-83242008000200007S1806-832420080002000072-s2.0-482490849902-s2.0-48249084990.pdf0000-0002-8159-4853Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Research1.223info:eu-repo/semantics/openAccess2024-01-25T06:28:50Zoai:repositorio.unesp.br:11449/70518Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:53:31.771671Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
title |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
spellingShingle |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel Rios, Daniela Dental enamel Scanning electron microscopy Tooth abrasion Tooth erosion adult animal beverage carbonated beverage cattle chemistry chewing gum dental care dental caries drug effect enamel female food human male pathology pH physiology saliva scanning electron microscopy surface property tooth brushing tooth disease ultrastructure Adult Animals Beverages Carbonated Beverages Cattle Chewing Gum Dental Caries Dental Enamel Dental Enamel Solubility Female Food Humans Hydrogen-Ion Concentration Male Microscopy, Electron, Scanning Saliva Surface Properties Tooth Abrasion Tooth Erosion Tooth Remineralization Toothbrushing Young Adult |
title_short |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
title_full |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
title_fullStr |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
title_full_unstemmed |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
title_sort |
Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel |
author |
Rios, Daniela |
author_facet |
Rios, Daniela Honório, Heitor Marques Magalhães, Ana Carolina da Silva, Salete Moura Bonifácio [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Machado, Maria Aparecida de Andrade Moreira Buzalaf, Marília Afonso Rabelo |
author_role |
author |
author2 |
Honório, Heitor Marques Magalhães, Ana Carolina da Silva, Salete Moura Bonifácio [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Machado, Maria Aparecida de Andrade Moreira Buzalaf, Marília Afonso Rabelo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Federal University of Alfenas Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rios, Daniela Honório, Heitor Marques Magalhães, Ana Carolina da Silva, Salete Moura Bonifácio [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Machado, Maria Aparecida de Andrade Moreira Buzalaf, Marília Afonso Rabelo |
dc.subject.por.fl_str_mv |
Dental enamel Scanning electron microscopy Tooth abrasion Tooth erosion adult animal beverage carbonated beverage cattle chemistry chewing gum dental care dental caries drug effect enamel female food human male pathology pH physiology saliva scanning electron microscopy surface property tooth brushing tooth disease ultrastructure Adult Animals Beverages Carbonated Beverages Cattle Chewing Gum Dental Caries Dental Enamel Dental Enamel Solubility Female Food Humans Hydrogen-Ion Concentration Male Microscopy, Electron, Scanning Saliva Surface Properties Tooth Abrasion Tooth Erosion Tooth Remineralization Toothbrushing Young Adult |
topic |
Dental enamel Scanning electron microscopy Tooth abrasion Tooth erosion adult animal beverage carbonated beverage cattle chemistry chewing gum dental care dental caries drug effect enamel female food human male pathology pH physiology saliva scanning electron microscopy surface property tooth brushing tooth disease ultrastructure Adult Animals Beverages Carbonated Beverages Cattle Chewing Gum Dental Caries Dental Enamel Dental Enamel Solubility Female Food Humans Hydrogen-Ion Concentration Male Microscopy, Electron, Scanning Saliva Surface Properties Tooth Abrasion Tooth Erosion Tooth Remineralization Toothbrushing Young Adult |
description |
This in situ study investigated, using scanning electron microscopy, the effect of stimulated saliva on the enamel surface of bovine and human substrates submitted to erosion followed by brushing abrasion immediately or after one hour. During 2 experimental 7-day crossover phases, 9 previously selected volunteers wore intraoral palatal devices, with 12 enamel specimens (6 human and 6 bovine). In the first phase, the volunteers immersed the device for 5 minutes in 150 ml of a cola drink, 4 times a day (8h00, 12h00, 16h00 and 20h00). Immediately after the immersions, no treatment was performed in 4 specimens (ERO), 4 other specimens were immediately brushed (0 min) using a fluoride dentifrice and the device was replaced into the mouth. After 60 min, the other 4 specimens were brushed. In the second phase, the procedures were repeated but, after the immersions, the volunteers stimulated the salivary flow rate by chewing a sugar-free gum for 30 min. Enamel superficial alterations of all specimens were then evaluated using a scanning electron microscope. Enamel prism core dissolution was seen on the surfaces submitted to erosion, while on those submitted to erosion and to abrasion (both at 0 and 60 min) a more homogeneous enamel surface was observed, probably due to the removal of the altered superficial prism layer. For all the other variables - enamel substrate and salivary stimulation the microscopic pattern of the enamel specimens was similar. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-04 2014-05-27T11:23:38Z 2014-05-27T11:23:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242008000200007 Brazilian Oral Research, v. 22, n. 2, p. 132-138, 2008. 1806-8324 1807-3107 http://hdl.handle.net/11449/70518 10.1590/S1806-83242008000200007 S1806-83242008000200007 2-s2.0-48249084990 2-s2.0-48249084990.pdf 0000-0002-8159-4853 |
url |
http://dx.doi.org/10.1590/S1806-83242008000200007 http://hdl.handle.net/11449/70518 |
identifier_str_mv |
Brazilian Oral Research, v. 22, n. 2, p. 132-138, 2008. 1806-8324 1807-3107 10.1590/S1806-83242008000200007 S1806-83242008000200007 2-s2.0-48249084990 2-s2.0-48249084990.pdf 0000-0002-8159-4853 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research 1.223 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
132-138 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129562770407424 |